Endophytic fungi are rich sources of structurally complex chemical scaffolds with interesting biological activities. However, their metabolome is still unknown, making them appealing for novel compound discovery. To maximize the number of secondary metabolites produced from a single microbial source, we used the "OSMAC (one strain-many compounds) approach." In potato dextrose medium, M. phaseolina produced phomeolic acid (1), ergosterol peroxide (2), and a volatile compound 1,4-benzene-diol. Incorporating an epigenetic modifier, sodium valproate, affected the metabolite profile of the fungus. It produced 3-acetyl-3-methyl dihydro-furan-2(3H)-one (3) and methyl-2-(methyl-thio)-butyrate (4), plus volatile chemicals: butylated hydroxy toluene (BHT), di-methyl-formamide, 3-amino-1-propanol, and 1,4-benzenediol, 2-amino-1-(O-methoxyphenyl) propane. The structure of compounds 1-4 was established with the help of spectroscopic data. This study revealed first-time compounds 1-4 in the fungus M. phaseolina using a classical and epigenetic manipulation approach.
- MeSH
- Ascomycota * metabolismus MeSH
- benzen metabolismus MeSH
- Brugmansia * MeSH
- butylhydroxytoluen metabolismus MeSH
- butyráty metabolismus MeSH
- endofyty chemie MeSH
- epigeneze genetická MeSH
- formamidy metabolismus MeSH
- furany metabolismus MeSH
- glukosa metabolismus MeSH
- kyselina valproová metabolismus MeSH
- propan metabolismus MeSH
- toluen metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
