Recent studies have shown that insects harbor numerous viruses of various taxa and that viral infections are often latent without noticeable symptoms. The red firebug Pyrrhocoris apterus, a true flightless bug from the family Pyrrhocoridae, is widely used for physiological studies on insect metabolism, endocrinology, and digestion. While exploring the transcriptome of P. apterus salivary glands, a nearly complete genomic sequence of a novel RNA virus was reconstructed. The virus, provisionally named Pyrrhocoris apterus virus 1 (PaV1), possesses eight potential open reading frames (ORFs) encoding for an array of proteins, some of which are involved in virus replication while others ensure success of the virus in multiple ways, including evasion of the host immune response. In addition to the information obtained from sequence analyses, we documented virus transmission, virus-induced mortality, host response upon persistent PaV1 infection, virion morphology, and putative virus-induced structures in salivary gland cells in a laboratory culture of red firebug. We propose that PaV1 belongs to a novel viral species of a new, yet-to-be established family.

• MeSH
• fylogeneze MeSH
• Heteroptera virologie   MeSH
• infekce RNA viry genetika   patofyziologie   přenos   MeSH
• RNA-viry fyziologie   MeSH
• virové geny genetika   MeSH
• virové proteiny analýza   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The complete genomic sequence of a new virus from cherry trees was determined. Its genome is 5857 nt long and resembles that of members of the genus Luteovirus in its genomic organization and nucleotide sequence. Based on the species demarcation criteria for luteoviruses, the virus represents a new luteovirus species. Furthermore, a 47-nt-long inverted repeat was found at the 3' end of its genome. The virus has been provisionally named cherry-associated luteovirus (ChALV) and is the fourth member of the family Luteoviridae reported to naturally infect woody plants.

• MeSH
• 3' přiléhající oblast DNA MeSH
• délka genomu MeSH
• fylogeneze * MeSH
• genom virový * MeSH
• Luteovirus klasifikace   genetika   izolace a purifikace   MeSH
• mapování chromozomů MeSH
• nemoci rostlin virologie   MeSH
• obrácené repetice MeSH
• otevřené čtecí rámce MeSH
• RNA virová genetika   MeSH
• sekvence nukleotidů MeSH
• slivoň švestka virologie   MeSH
• Publikační typ
• časopisecké články MeSH