Staphylococcus aureus is one of the most important pathogens causing chronic biofilm infections. These are becoming more difficult to treat owing to drug resistance, particularly because S. aureus biofilms limit the efficacy of antimicrobial agents, leading to high morbidity and mortality. In the present study, we screened for inhibitors of S. aureus biofilm formation using a natural product library from the Korea Chemical Bank (KCB). Screening by crystal violet-based biomass staining assay identified hit compounds. Further examination of antibiofilm properties of these compounds was conducted and led to the identification of celastrol and telithromycin. In vitro, both celastrol and telithromycin were toxic to planktonic S. aureus and also active against a clinical methicillin-resistant S. aureus (MRSA) isolate. The effect of the compounds on preformed biofilms of clinical MRSA isolates was evaluated by confocal laser scanning microscopy (CLSM), which revealed the absence of typical biofilm architecture. In addition, celastrol and telithromycin inhibited the production of extracellular protein at selected sub-MIC concentrations, which revealed the reduced extracellular polymeric substance (EPS) secretion. Celastrol exhibited greater cytotoxicity than telithromycin. These data suggest that the hit compounds, especially telithromycin, could be considered novel inhibitors of S. aureus biofilm. Although the mechanisms of the effects on S. aureus biofilms are not fully understood, our data suggest that telithromycin could be a useful adjuvant therapeutic agent for S. aureus biofilm-related infections.
- MeSH
- antibakteriální látky farmakologie MeSH
- biofilmy účinky léků růst a vývoj MeSH
- biologické přípravky farmakologie MeSH
- genciánová violeť MeSH
- ketolidy farmakologie MeSH
- knihovny malých molekul farmakologie MeSH
- methicilin rezistentní Staphylococcus aureus účinky léků fyziologie MeSH
- mikrobiální testy citlivosti MeSH
- plankton účinky léků růst a vývoj MeSH
- rychlé screeningové testy MeSH
- Staphylococcus aureus účinky léků fyziologie MeSH
- triterpeny farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
