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Autor
Chiu, Charles 1 Dacks, Joel B 1 Eliáš, Marek 1 Fritz-Laylin, Lillian 1 Ginger, Michael L 1 Greninger, Alex 1 Herman, Emily K 1 MacIntyre, Georgina 1 Marciano-Cabral, Francine 1 Miller, Haylea C 1 Morgan, Matthew J 1 Muller, Norbert 1 Najle, Sebastian Rodrigo 1 Puzon, Geoffrey J 1 Ramirez-Macias, Inmaculada 1 Slamovits, Claudio H 1 Tsaousis, Anastasios D 1 Vargová, Romana 1 Velle, Katrina 1 Walsh, Tom 1
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Pracoviště
CSIRO Indian Oceans Marine Research C... 1 CSIRO Land and Water Black Mountain L... 1 CSIRO Land and Water Centre for Envir... 1 Center for Autoimmune Genomics and Et... 1 Centre for Genomic Regulation 08003 B... 1 Centre for Organelle Research Departm... 1 Department of Agricultural Food and N... 1 Department of Biochemistry and Molecu... 1 Department of Biology University of M... 1 Department of Biology and Ecology Fac... 1 Department of Cardiology Hospital Cli... 1 Department of Laboratory Medicine Uni... 1 Department of Life Sciences The Natur... 1 Department of Medicine Faculty of Med... 1 Department of Microbiology and Immuno... 1 Department of Ophthalmology Inselspit... 1 Department of Pediatrics University o... 1 Division of Infectious Disease Depart... 1 Faculty of Science Charles University... 1 Institut de Biologia Evolutiva Barcel... 1
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Autor
Chiu, Charles 1 Dacks, Joel B 1 Eliáš, Marek 1 Fritz-Laylin, Lillian 1 Ginger, Michael L 1 Greninger, Alex 1 Herman, Emily K 1 MacIntyre, Georgina 1 Marciano-Cabral, Francine 1 Miller, Haylea C 1 Morgan, Matthew J 1 Muller, Norbert 1 Najle, Sebastian Rodrigo 1 Puzon, Geoffrey J 1 Ramirez-Macias, Inmaculada 1 Slamovits, Claudio H 1 Tsaousis, Anastasios D 1 Vargová, Romana 1 Velle, Katrina 1 Walsh, Tom 1
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Pracoviště
CSIRO Indian Oceans Marine Research C... 1 CSIRO Land and Water Black Mountain L... 1 CSIRO Land and Water Centre for Envir... 1 Center for Autoimmune Genomics and Et... 1 Centre for Genomic Regulation 08003 B... 1 Centre for Organelle Research Departm... 1 Department of Agricultural Food and N... 1 Department of Biochemistry and Molecu... 1 Department of Biology University of M... 1 Department of Biology and Ecology Fac... 1 Department of Cardiology Hospital Cli... 1 Department of Laboratory Medicine Uni... 1 Department of Life Sciences The Natur... 1 Department of Medicine Faculty of Med... 1 Department of Microbiology and Immuno... 1 Department of Ophthalmology Inselspit... 1 Department of Pediatrics University o... 1 Division of Infectious Disease Depart... 1 Faculty of Science Charles University... 1 Institut de Biologia Evolutiva Barcel... 1
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Herman, Emily K
Autor Herman, Emily K Division of Infectious Disease, Department of Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada. eherman@ualberta.ca Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada. eherman@ualberta.ca
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Greninger, Alex
Autor Greninger, Alex Laboratory Medicine and Medicine / Infectious Diseases, UCSF-Abbott Viral Diagnostics and Discovery Center, UCSF Clinical Microbiology Laboratory UCSF School of Medicine, San Francisco, USA Department of Laboratory Medicine, University of Washington Medical Center, Montlake, USA
- van der Giezen, Mark
- Ginger, Michael L
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Ramirez-Macias, Inmaculada
Autor Ramirez-Macias, Inmaculada Division of Infectious Disease, Department of Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada Department of Cardiology, Hospital Clinico Universitario Virgen de la Arrixaca. Instituto Murciano de Investigación Biosanitaria. Centro de Investigación Biomedica en Red-Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain
- Miller, Haylea C
- Morgan, Matthew J
- Tsaousis, Anastasios D
- Velle, Katrina
- Vargová, Romana
BioMedCentral Open Access od 2003
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Springer Journals Complete - Open Access od 2003-12-01
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PubMed
34294116
DOI
10.1186/s12915-021-01078-1
Knihovny.cz E-zdroje
BACKGROUND: The opportunistic pathogen Naegleria fowleri establishes infection in the human brain, killing almost invariably within 2 weeks. The amoeba performs piece-meal ingestion, or trogocytosis, of brain material causing direct tissue damage and massive inflammation. The cellular basis distinguishing N. fowleri from other Naegleria species, which are all non-pathogenic, is not known. Yet, with the geographic range of N. fowleri advancing, potentially due to climate change, understanding how this pathogen invades and kills is both important and timely. RESULTS: Here, we report an -omics approach to understanding N. fowleri biology and infection at the system level. We sequenced two new strains of N. fowleri and performed a transcriptomic analysis of low- versus high-pathogenicity N. fowleri cultured in a mouse infection model. Comparative analysis provides an in-depth assessment of encoded protein complement between strains, finding high conservation. Molecular evolutionary analyses of multiple diverse cellular systems demonstrate that the N. fowleri genome encodes a similarly complete cellular repertoire to that found in free-living N. gruberi. From transcriptomics, neither stress responses nor traits conferred from lateral gene transfer are suggested as critical for pathogenicity. By contrast, cellular systems such as proteases, lysosomal machinery, and motility, together with metabolic reprogramming and novel N. fowleri proteins, are all implicated in facilitating pathogenicity within the host. Upregulation in mouse-passaged N. fowleri of genes associated with glutamate metabolism and ammonia transport suggests adaptation to available carbon sources in the central nervous system. CONCLUSIONS: In-depth analysis of Naegleria genomes and transcriptomes provides a model of cellular systems involved in opportunistic pathogenicity, uncovering new angles to understanding the biology of a rare but highly fatal pathogen.
- MeSH
- genomika MeSH
- modely nemocí na zvířatech MeSH
- myši MeSH
- Naegleria fowleri * genetika MeSH
- transkriptom MeSH
- trogocytóza MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
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