P38alpha kinase plays an important role in the regulation of both cell stress response and cell fate. In this study, we report that p38alpha kinase-deficient embryonic stem cells exhibit a higher production of reactive oxygen species (ROS) in contrast to their wild-type counterpart. Analysis of the expressions of NADPH oxidases (NOXs) and dual oxidases, crucial enzymes involved in intracellular ROS formation, shows NOX2/gp91phox is over-expressed in p38alpha deficient cells. The particular increase in superoxide formation was confirmed by the specific detection of hydroethidine derivate 2-hydroxyethidium. ROS formation decreased when the level of NOX2 was silenced by siRNA in p38alpha deficient cells. These data suggest the importance of p38alpha kinase in the regulation of ROS metabolism in embryonic stem cells and the significance of the observed phenomena of cancer cell-like phenotypes, which is discussed.
- MeSH
- buněčná diferenciace fyziologie MeSH
- genový knockdown MeSH
- genový knockout MeSH
- kultivované buňky MeSH
- membránový potenciál mitochondrií fyziologie MeSH
- mitochondrie metabolismus MeSH
- mitogenem aktivovaná proteinkinasa 14 genetika metabolismus MeSH
- myší embryonální kmenové buňky metabolismus MeSH
- myši MeSH
- NADPH-oxidasa 2 genetika metabolismus MeSH
- superoxidy metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
