Adjuvant therapy and radiotherapy improves the survival of patients with metastatic and locally advanced gastric cancer (GC). However, the resistance to radiotherapy limits its clinical usage. Rhotekin 2 (RTKN2) functions as an oncogene and confers resistance to ultraviolet B-radiation and apoptosis- inducing agents. Here, the role of RTKN2 in radiosensitivity of GC cell lines was investigated. RTKN2 was found to be elevated in GC tissues and cells. A series of functional assays revealed that overexpression of RTKN2 induced GC cell proliferation, promoted GC cell migration and invasion, while inhibiting GC cell apoptosis. However, silence of RTKN2 promoted GC cell apoptosis, while repressing GC cell proliferation, invasion and migration. GC cells were exposed to irradiation, and data from cell survival and apoptotic assays showed that knock-down of RTKN2 enhanced radiosensitivity of GC through up-regulation of apoptosis and down-regulation of proliferation in irradiation-exposed GC cells. Moreover, the protein expression of β-catenin and c-Myc in GC cells was enhanced by RTKN2 over-expression, but reduced by RTKN2 silence. Interference of RTKN2 down-regulated nuclear β-catenin expression, while up-regulating cytoplasmic β-catenin in GC. In conclusion, RTKN2 contributed to cell growth and radioresistance in GC through activation of Wnt/β-catenin signalling.
- MeSH
- beta-katenin metabolismus MeSH
- intracelulární signální peptidy a proteiny metabolismus MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádory žaludku * genetika patologie radioterapie MeSH
- pohyb buněk MeSH
- proliferace buněk MeSH
- regulace genové exprese u nádorů MeSH
- signální dráha Wnt MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The increased proliferation and migration of airway smooth muscle cells (ASMCs) is a key process in the formation of airway remodeling in asthma. In this study, we focused on the expression of mircoRNA-18a (miR-18a) in airway remodeling in bronchial asthma and its related mechanisms. ASMCs are induced by platelet-derived growth factor BB (PDGF-BB) for in vitro airway remodeling. The expression of miR-18a in sputum of asthmatic patients and healthy volunteers was detected by qRT-PCR. The expression of miR-18a was over-expressed or interfered with in PDGF-BB-treated ASMCs. Cell proliferation, apoptosis and migration were detected by MTT, flow cytometry and Transwell, respectively; the expression of contractile phenotype marker proteins (SM-22?, ?-SM-actin, calponin) and key molecules of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway (PI3K, p-PI3K, AKT and p-AKT) in ASMCs were detected by Western blot. The expression of miR-18a was down-regulated in the sputum and PDGF-BB-treated ASMCs of asthma patients. PDGF-BB could promote the proliferation and migration of ASMCs and inhibit their apoptosis; it could also promote the phenotypic transformation of ASMCs and activate the PI3K/AKT pathway. MiR-18a could inhibit the proliferation, migration ability and phenotypic transformation of ASMCs induced by PDGF-BB to a certain extent and alleviate the effect of PDGF-BB in supressing apoptosis, while miR-18a could inhibit the activation of the PI3K/AKT pathway. MiR-18a inhibits PDGF-BB-induced proliferation, migration and phenotypic conversion of ASMCs by inhibiting the PI3K/AKT pathway, thus attenuating airway remodeling in asthma.
- MeSH
- becaplermin metabolismus MeSH
- bronchiální astma metabolismus MeSH
- dospělí MeSH
- fosfatidylinositol-3-kinasy metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA metabolismus MeSH
- mladiství MeSH
- mladý dospělý MeSH
- myocyty hladké svaloviny fyziologie MeSH
- protoonkogenní proteiny c-akt metabolismus MeSH
- remodelace dýchacích cest * MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- signální transdukce MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Spiroplasma eriocheiris caused massive mortality of Chinese mitten crab Eriocheir sinensis but little is known about the molecular characteristics of this microorganism. We described here the identification of a spiralin-like protein (SLP31) from S. eriocheiris and expression in Escherichia coli. Analysis of the nucleotide sequence revealed that the clone has an open reading frame of 837 bp encoding a protein of 279 amino acids. Theoretical isoelectric point and molar mass for SLP31 are 7.72 and 31 kDa, respectively. The similarity of SLP31 deduced amino acid sequence shared with the spiralin from other species indicated that the gene may be a member of spiralin family. The TGA codon in Spiroplasma serves not as a stop signal but as a code for the amino acid tryptophan. After cloning the SLP31, the gene was site-mutated from TGA to TGG and transcribed in E. coli to full expression of SLP31. The purified recombinant protein was used to determine the immune reactivity by Western blotting which suggests that SLP31 could be a good antigen for immunodiagnostic of tremor disease in E. sinensis.
- MeSH
- antigeny bakteriální MeSH
- bakteriologické techniky metody MeSH
- DNA bakterií genetika chemie MeSH
- Escherichia coli genetika MeSH
- financování organizované MeSH
- fylogeneze MeSH
- imunoanalýza metody MeSH
- izoelektrický bod MeSH
- klonování DNA MeSH
- krabi mikrobiologie MeSH
- molekulární sekvence - údaje MeSH
- molekulová hmotnost MeSH
- otevřené čtecí rámce MeSH
- proteiny vnější bakteriální membrány MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie MeSH
- shluková analýza MeSH
- Spiroplasma genetika imunologie izolace a purifikace MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Geografické názvy
- Čína MeSH
As one of the most important bioleaching bacterial species, Acidithiobacillus ferrooxidans is subjected to different kinds of environmental stress such as temperature changes, presence of some toxic heavy metals or pH changes, which normally take place in bioleaching operations and may affect the activity of the bacteria. To obtain further knowledge of the protective mechanisms employed by A. ferrooxidans in response to elevated growth temperature, temporal gene expression profiles were examined in cells subjected to heat stress by using real-time PCR. The majority of predicted genes, including those encoding chaperones and heat-shock proteins, were significantly up-regulated (P < 0.05 and the fold change >or= 2.0) over a 25-min period after upshift to the heatshock temperature (from 30 to 42 degrees C) and then decrease to readjust their transcript levels to a new steady state at 42 degrees C. Finally, based on the gene expression data and computational analysis, a putative regulatory site having T-T-T-T-T-T-n-n in the -35 region and n-T-A-T-n-A-T-C in the -10 region with average 19 base pairs separating the two elements was predicted upstream of a number of heat-inducible genes.
- MeSH
- Acidithiobacillus fyziologie genetika MeSH
- bakteriální proteiny fyziologie genetika MeSH
- financování organizované MeSH
- genom bakteriální genetika MeSH
- koncentrace vodíkových iontů MeSH
- polymerázová řetězová reakce metody MeSH
- promotorové oblasti (genetika) imunologie MeSH
- proteiny tepelného šoku fyziologie genetika MeSH
- regulace genové exprese u bakterií fyziologie genetika MeSH
- teplota MeSH
- výpočetní biologie MeSH
- MeSH
- apolipoproteiny A krev MeSH
- apolipoproteiny B krev MeSH
- apolipoproteiny C krev MeSH
- diabetes mellitus 2. typu MeSH
- dítě MeSH
- epidemiologické studie MeSH
- Indiáni Jižní Ameriky dějiny etnologie MeSH
- inzulinová rezistence MeSH
- lidé MeSH
- lipoproteiny HDL MeSH
- metabolický syndrom MeSH
- mladiství MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- MeSH
- apoptóza genetika MeSH
- finanční podpora výzkumu jako téma MeSH
- inzulin sekrece MeSH
- sulfan farmakologie metabolismus MeSH
- Publikační typ
- kongresy MeSH
