Plants of the genus Pleione, originating from hobby growers in the Netherlands and in the Czech Republic, were conspicuous for viral infection, showing symptoms of leaf mosaic or flower breaking. Using Sanger and high throughput sequencing, the full genome sequence of a novel potyvirus was obtained from sequencing data. The genome sequence was annotated and compared to the genome of other potyviruses. The virus was experimentally transmitted by aphids into Pleione and Chenopodium quinoa plants. The name Pleione flower breaking virus (PlFBV) was suggested for the new virus. The presence of the virus was confirmed using RT-PCR, with newly designed primers targeting this new species. The incidence of the virus was contrasted between both countries and might have been influenced by the growth conditions and the exposure of the plants to aphids.

• MeSH
• Molecular Sequence Annotation MeSH
• Chenopodium quinoa virology   MeSH
• Insect Vectors MeSH
• Incidence MeSH
• Aphids MeSH
• Plant Diseases virology   MeSH
• Orchidaceae virology   MeSH
• Reverse Transcriptase Polymerase Chain Reaction MeSH
• Potyvirus classification   genetics   isolation & purification   MeSH
• Disease Transmission, Infectious MeSH
• Sequence Analysis, DNA MeSH
• Whole Genome Sequencing MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Czech Republic MeSH
• Netherlands MeSH