KEY MESSAGE: Using COS markers, the study reveals homeologous relationships between tetraploid Agropyron cristatum and bread wheat to support alien introgression breeding of wheat. Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat that possesses many genes that are potentially useful in wheat improvement. The species comprises a complex of diploid, tetraploid and hexaploid forms. In this study, wheat-A. cristatum chromosome, telosome and translocation lines were used to characterize syntenic relationships between tetraploid A. cristatum and bread wheat. Prior to mapping COS markers, the cytogenetic stock lines were characterized for fertility and by FISH and GISH for karyotype stability. Out of 328 COS markers selected for the study, 279 consistently amplified products in tetraploid A. cristatum, and, out of these, 139 were polymorphic between tetraploid crested wheatgrass and wheat. Sixty-nine markers were found to be suitable for the detection of tetraploid A. cristatum chromosomes 1P-6P in wheat, ranging from 6 to 17 markers per chromosome. BLASTn of the source ESTs resulted in significant hits for 67 markers on the wheat pseudomolecules. Generally, COS markers of the same homeologous group were detected on similar arms in both Agropyron and wheat. However, some intragenomic duplications and chromosome rearrangements were detected in tetraploid A. cristatum. These results provide new insights into the structure and evolution of the tetraploid A. cristatum genome and will facilitate the exploitation of the wild species for introgression breeding of bread wheat.
- MeSH
- Agropyron genetika růst a vývoj MeSH
- chléb analýza MeSH
- chromozomy rostlin MeSH
- genetické markery * MeSH
- genom rostlinný genetika MeSH
- genotyp MeSH
- hybridizace genetická * MeSH
- mapování chromozomů MeSH
- pšenice genetika růst a vývoj MeSH
- tetraploidie * MeSH
- translokace genetická MeSH
- Publikační typ
- časopisecké články MeSH
KEY MESSAGE: Fluorescence in situ hybridization with probes for 45 cDNAs and five tandem repeats revealed homoeologous relationships of Agropyron cristatum with wheat. The results will contribute to alien gene introgression in wheat improvement. Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat and a promising source of novel genes for wheat improvement. To date, identification of A. cristatum chromosomes has not been possible, and its molecular karyotype has not been available. Furthermore, homoeologous relationship between the genomes of A. cristatum and wheat has not been determined. To develop chromosome-specific landmarks, A. cristatum genomic DNA was sequenced, and new tandem repeats were discovered. Their distribution on mitotic chromosomes was studied by fluorescence in situ hybridization (FISH), which revealed specific patterns for five repeats in addition to 5S and 45S ribosomal DNA and rye subtelomeric repeats pSc119.2 and pSc200. FISH with one tandem repeat together with 45S rDNA enabled identification of all A. cristatum chromosomes. To analyze the structure and cross-species homoeology of A. cristatum chromosomes with wheat, probes for 45 mapped wheat cDNAs covering all seven chromosome groups were localized by FISH. Thirty-four cDNAs hybridized to homoeologous chromosomes of A. cristatum, nine hybridized to homoeologous and non-homoeologous chromosomes, and two hybridized to unique positions on non-homoeologous chromosomes. FISH using single-gene probes revealed that the wheat-A. cristatum collinearity was distorted, and important structural rearrangements were observed for chromosomes 2P, 4P, 5P, 6P and 7P. Chromosomal inversions were found for pericentric region of 4P and whole chromosome arm 6PL. Furthermore, reciprocal translocations between 2PS and 4PL were detected. These results provide new insights into the genome evolution within Triticeae and will facilitate the use of crested wheatgrass in alien gene introgression into wheat.
- MeSH
- Agropyron MeSH
- Crataegus MeSH
- Equisetum MeSH
- fytoterapie metody využití MeSH
- hypertenze farmakoterapie komplikace MeSH
- kardiovaskulární nemoci farmakoterapie prevence a kontrola MeSH
- léčivé rostliny MeSH
- lidé MeSH
- Matricaria MeSH
- Olea MeSH
- Polygonum MeSH
- řebříček MeSH
- Viscum album MeSH
- Vitis MeSH
- Check Tag
- lidé MeSH
Akutní toxické hepatitidy mohou být následkem užívání řady léků, jsou však popsány i po bylinných přípravcích. V naší kazuistice prezentujeme případ akutní toxické hepatitidy po užívání bylinného doplňku stravy za účelem léčby psoriázy. I přes rozšířený mýtus o neškodnosti mohou bylinné přípravky stejně jako „klasické" léky vyvolávat závažné nežádoucí účinky.
- MeSH
- Agropyron škodlivé účinky MeSH
- bříza škodlivé účinky MeSH
- gama-glutamyltransferasa diagnostické užití metabolismus MeSH
- jalovec (rod) škodlivé účinky MeSH
- lékové postižení jater diagnóza etiologie terapie MeSH
- ostropestřec mariánský škodlivé účinky MeSH
- potravní doplňky využití MeSH
- psoriáza farmakoterapie MeSH
- rostlinné přípravky škodlivé účinky terapeutické užití toxicita MeSH
- Publikační typ
- kazuistiky MeSH
