Stereospecific nucleation of mesoporous hybrid microspheres composed of CaCO3 and carrageenan was appended to one side of bacterial cellulose membrane synthesized in static cultures of Gluconacetobacter hansenii to develop an implantable drug delivery device. The synthesis of the hybrid microparticles proceeds by self-assembly mechanism in the presence of calcium and contains tailorable amounts of doxorubicin. However, in the absence of the particles, doxorubicin was distributed along the BC film, but without control release of drug. Infrared spectroscopy, confocal and scanning electron microscopies analyses demonstrate that the doxorubicin is entrapped inside the hybrid particles with approximately 80% drug loading compared to the 11% obtained for native bacterial cellulose. Doxorubicin content in the hybrid particles can be increased by a factor of 10 (from 258.6 to 2586.3 nmol ml−1), and also by the quantities of particles regulated by the CaCO3–carrageenan content and the physicochemical microenvironment. The hybrid BC system can be considered as smart device since the kinetic release of doxorubicin from the hybrid cellulose system rise from 1.50 to 2.75 μg/membrane/day when the pH decreases from 7.4 to 5.8 at 37 °C, a pathologic simulated environment. The hybrid microparticle system can be potentially used as an implantable drug delivery system for personalized oncological therapies.
- MeSH
- antitumorózní látky aplikace a dávkování terapeutické užití toxicita MeSH
- bakteriální polysacharidy biosyntéza farmakologie metabolismus MeSH
- biomedicínský výzkum metody trendy MeSH
- celulosa * biosyntéza farmakologie MeSH
- doxorubicin aplikace a dávkování farmakologie MeSH
- elektronová mikroskopie metody využití MeSH
- Gluconacetobacter růst a vývoj účinky léků MeSH
- individualizovaná medicína metody trendy využití MeSH
- karagenan * biosyntéza metabolismus MeSH
- konfokální mikroskopie metody využití MeSH
- lidé MeSH
- nanokompozity chemie mikrobiologie využití MeSH
- spektrofotometrie infračervená metody využití MeSH
- statistika jako téma metody MeSH
- systémy cílené aplikace léků metody využití MeSH
- techniky in vitro MeSH
- uhličitan vápenatý * chemická syntéza metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
