Data on the substantial physiological role of the immune system in the organism's ability to manage proper differentiation and function of normal tissues (tissue homeostasis), and detailed causes of the immune system's essential role for the in-vivo stimulation of cancer growth, are severely lacking. This results in a lack of effective cancer immunotherapy without adverse events, and in the lack of long-lasting cancer immune prophylaxes, particularly in ovarian cancers. Elimination of blood auto-antibodies blocking anti-cancer T cell effectors by intermittent moderate doses of cyclophosphamide, facilitation of the immune system reactivity against alloantigens of cancer cells by two subsequent blood transfusions, and augmentation of anticancer immunity by weekly intradermal injections of bacterial toxins, caused during the subsequent treatment-free period, lasting for two to four weeks, regression of inoperable epithelial ovarian cancers and regeneration of the tremendously metastatically altered abdominal tissues into normal healthy conditions without multivisceral cytoreductive surgery, which can result in life-threatening consequences. An otherwise untreated rectal cancer, progressing over 3 years, regressed after severe toxic dermatitis lasting over one week. This was caused by an accidental consumption of a large raw shiitake mushroom. Subsequent daily consumptions of 2 g Metformin ER and honeybee propolis ethanol extract, and weekly single larger raw shiitake mushroom, which all stimulate immune system reactivity against cancer stem cells, prevented malignant recurrence over the next 29 years without recurring dermatitis, and maintained healthy organism's conditions. These observations indicate that regression of advanced inoperable cancers and long-lasting cancer immune prophylaxis can be reached by simple approaches.
- MeSH
- autoprotilátky imunologie MeSH
- epiteliální ovariální karcinom imunologie patologie terapie MeSH
- homeostáza MeSH
- houby šii-take MeSH
- imunitní systém MeSH
- imunoterapie metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- lokální recidiva nádoru MeSH
- metformin terapeutické užití MeSH
- nádory rekta imunologie patologie terapie MeSH
- nádory vaječníků imunologie patologie terapie MeSH
- proliferace buněk MeSH
- propolis MeSH
- T-lymfocyty imunologie MeSH
- včely MeSH
- výsledek terapie MeSH
- zvířata MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- přehledy MeSH
Autor popisuje případ 39letého muže, u kterého se objevily projevy flagelátní dermatitidy po požití syrové houby shiitake provázené celkovými příznaky podobné nachlazení a střevní viróze. Odebraná infekční sérologie a biochemie nevykazovaly významnější patologické hodnoty. Léčba celkovým kortikoidy po 4 dny, užívání antihistaminik a aplikace lokální terapie vedly k ústupu všech obtíží. Článek poskytuje současné poznatky o dermatitis flagellata a její patogenezi včetně role a možných užítí β-glukanů extrahovaných z hub.
The author describes a case of a 39-year-old man, who developed symptoms of flagellate dermatitis accompanied by flu-like symptoms and symptoms of gastroenteritis after consuming a raw shiitake mushroom. Neither infectious serology nor biochemistry showed significant pathological values. The treatment with systemic corticosteroids for four days, antihistamines and local therapy led to remission of all symptoms. The article provides current knowledge of flagellate dermatitis and its pathogenesis, including the role and possible uses of β-glucans extracted from fungi.
- Klíčová slova
- flagelátní dermatitida,
- MeSH
- antihistaminika terapeutické užití MeSH
- beta-glukany farmakologie terapeutické užití MeSH
- dermatitida * diagnóza etiologie terapie MeSH
- dospělí MeSH
- houby šii-take * chemie patogenita MeSH
- hydrokortison aplikace a dávkování terapeutické užití MeSH
- lidé MeSH
- prednison aplikace a dávkování terapeutické užití MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
The antimicrobial, cytotoxic, anti-inflammatory, and antioxidant properties of aqueous extracts of raw and culinary processed shiitake mushrooms were evaluated and compared with those of lenthionine (1,2,3,5,6-penta-thiepane), the principal aroma-bearing substance of the shiitake medicinal mushroom (Lentinus edodes). Antimicrobial activity was tested using a panel of 4 strains of bacteria, 2 yeasts, and 2 fungi. Cytotoxic properties were evaluated against 3 cell lines (HepG2, HeLa, PaTu), whereas the anti-inflammatory activity of tested samples was assayed based on their ability to attenuate the secretion of the cytokine tumor necrosis factor-α. Antioxidant activity was measured using in vitro DPPH and ABTS assays. It was found that lenthionine possesses significant antimicrobial properties; it is remarkably effective in inhibiting the growth of yeasts and fungi (minimum inhibitory concentration, 2-8 μg/mL) and thus is comparable to standard antifungal agents. Lenthionine is also able to decrease significantly the production of tumor necrosis factor-a and thus could be at least partly responsible for the observed anti-inflammatory effect of shiitake. On the other hand, lenthionine does not seem to contribute significantly to the well-known anticancer and antioxidant effects of the mushroom.
- MeSH
- antibakteriální látky izolace a purifikace farmakologie MeSH
- antiflogistika izolace a purifikace farmakologie MeSH
- antifungální látky izolace a purifikace farmakologie MeSH
- antioxidancia izolace a purifikace farmakologie MeSH
- Bacteria účinky léků MeSH
- buňky Hep G2 MeSH
- cytokiny účinky léků MeSH
- cytotoxiny izolace a purifikace farmakologie MeSH
- HeLa buňky MeSH
- houby šii-take chemie MeSH
- houby účinky léků MeSH
- kvasinky účinky léků MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- nádorové buněčné linie MeSH
- thiepiny chemie farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Lentinula edodes (houževnatec jedlý) je stopkovýtrusá dřevokazná houba, známá pod japonským jménem šiitake. Houba je jedlá a odedávna se používá také v tradiční medicíně k léčbě mnoha onemocnění. V současné době probíhá řada studií, které její účinky potvrzují. Perspektivní je především protinádorový, imunomodulační a antimikrobiální účinek.
Lentinula edodes is a basidiomycetous wood-decaying fungus, known as shiitake. Besides being edible, Lentinula has been used for thousands of years in traditional medicine for treating many illnesses. Recent studies confirm its healing effects. The most promising is its antitumor, immunomodulatory and antimicrobial activity.
- Klíčová slova
- houževnatec jedlý,
- MeSH
- antibakteriální látky MeSH
- antikarcinogenní látky MeSH
- antioxidancia MeSH
- fytoterapie * MeSH
- houby šii-take * terapeutické užití MeSH
- hypolipidemika MeSH
- imunologické faktory MeSH
- lentinan farmakologie terapeutické užití MeSH
- lidé MeSH
- plodnice hub MeSH
- potravní doplňky MeSH
- Check Tag
- lidé MeSH
- Klíčová slova
- geriatrika, adaptogeny, Panax ginseng, Eleuterococcus senticosus, Lentinula edodes, Ganoderma lucidum, Lycium chinense, Leuzea carthamoides, Schisandra chinensis,
- MeSH
- eleuterokok MeSH
- farmakoterapie MeSH
- fytoterapie * MeSH
- geriatrie * MeSH
- houby šii-take MeSH
- kustovnice MeSH
- léčivé rostliny MeSH
- Leuzea MeSH
- lidé MeSH
- potravní doplňky * MeSH
- reishi MeSH
- Schisandra MeSH
- senioři MeSH
- ženšen MeSH
- Check Tag
- lidé MeSH
- senioři MeSH
Liečivé rastliny, ISSN 1335-9878 Roč. 51, č. 3, príloha octóber 2014
7 stran : ilustrace ; 21 cm
- MeSH
- Agaricales MeSH
- aminokyseliny MeSH
- Basidiomycota MeSH
- cytokiny MeSH
- fytoterapie MeSH
- fyziologie výživy imunologie MeSH
- glukany MeSH
- imunomodulace MeSH
- Lentinula MeSH
- nutriční terapie MeSH
- pěstování plodin MeSH
- Publikační typ
- periodika MeSH
- populární práce MeSH
- Konspekt
- Fyzioterapie. Psychoterapie. Alternativní lékařství
- NLK Obory
- alternativní lékařství
Melanins are complex natural pigments that darken the skin and are difficult to degrade. This study evaluated synthetic melanin decolorization by the crude laccase from fungus Lentinus polychrous in the absence and presence of selected redox mediators. The greatest melanin decolorization activity was 87 % at pH 6.5 within 3 h in the presence of 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) diammonium salt (ABTS), whereas only about 22 % melanin decolorized at pH 5.0 in case of no mediator. The optimum temperatures for melanin decolorization in the absence and presence of ABTS were 55 and 35°C, respectively. Using a natural redox mediator, 1.0 mmol/L vanillin leads to 45 % melanin decolorization. Our results suggest the possibility of applying vanillin for L. polychrous laccase-catalyzed decolorization of melanin.
- MeSH
- dermatitida etiologie patologie MeSH
- houby šii-take chemie MeSH
- kůže patologie MeSH
- lentinan škodlivé účinky MeSH
- lidé středního věku MeSH
- lidé MeSH
- potravinová alergie etiologie patologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- dopisy MeSH
- kazuistiky MeSH
Aim of this work was to investigate the ability of Lentinus (Panus) tigrinus to degrade and detoxify a chlorobenzoate (CBA) mixture composed of mono-, di- and tri-chlorinated isomers. The degradation process was investigated as a function of both the growing medium (i.e. low N Kirk's and malt extract-glucose medium) and cultivation conditions (i.e. stationary and shaken cultures). The majority of CBAs were quantitatively degraded within the early 15 d from spiking with the notable exception of the double ortho-chlorinated compounds, 2,6-di-, 2,3,6-tri- and 2,4,6-tri-CBA. Analysis of the degradation intermediates indicated the occurrence of side chain reduction, hydroxylation and methylation reactions. Although CBAs stimulated laccase production, in vitro experiments with a purified L. tigrinus laccase isoenzyme demonstrated its inability to participate in the initial attack on CBAs even in the presence of redox mediators; similar results were found with a Mn-peroxidase isoenzyme. Conversely, prompt degradation was observed upon 1h incubation of CBAs with a purified microsomal fraction containing cytochrome P-450 monooxygenase. The nature of some reaction products (i.e. hydroxylated derivatives), the dependency of the reaction on NADPH and its susceptibility to either CO or piperonyl butoxide inhibition confirmed the involvement of L. tigrinus cytochrome P-450 in the early steps of CBA degradation.
- MeSH
- Aliivibrio fischeri metabolismus MeSH
- biodegradace MeSH
- biomasa MeSH
- chemické látky znečišťující vodu analýza metabolismus MeSH
- chlor chemie MeSH
- chlorbenzoáty analýza metabolismus MeSH
- čištění vody metody MeSH
- kultivační média metabolismus MeSH
- kyslík chemie MeSH
- lakasa metabolismus MeSH
- Lentinula metabolismus MeSH
- luminiscence MeSH
- mikrozomy metabolismus MeSH
- peroxidasy MeSH
- substrátová specifita MeSH
- systém (enzymů) cytochromů P-450 metabolismus MeSH
- testy toxicity MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
