In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-time PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. This way, the presence of genetically modified petunia was demonstrated via the characterization of their transgene flanking regions as well as unnatural associations of elements from their transgenic cassette.
- MeSH
- Evropská unie MeSH
- geneticky modifikované rostliny MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- nanopóry MeSH
- Petunia klasifikace genetika MeSH
- procházení chromozomu metody MeSH
- sekvenční analýza DNA přístrojové vybavení MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Maďarsko MeSH
