In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-time PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. This way, the presence of genetically modified petunia was demonstrated via the characterization of their transgene flanking regions as well as unnatural associations of elements from their transgenic cassette.
- MeSH
- European Union MeSH
- Plants, Genetically Modified MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Nanopores MeSH
- Petunia classification genetics MeSH
- Chromosome Walking methods MeSH
- Sequence Analysis, DNA instrumentation MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
- Hungary MeSH
