BACKGROUND AND AIMS: Allelopathy may drive invasions of some exotic plants, although empirical evidence for this theory remains largely inconclusive. This could be related to the large intraspecific variability of chemically mediated plant-plant interactions, which is poorly studied. This study addressed intraspecific variability in allelopathy of Heracleum mantegazzianum (giant hogweed), an invasive species with a considerable negative impact on native communities and ecosystems. METHODS: Bioassays were carried out to test the alleopathic effects of H. mantegazzianum root exudates on germination of Arabidopsis thaliana and Plantago lanceolata. Populations of H. mantegazzianum from the Czech Republic were sampled and variation in the phytotoxic effects of the exudates was partitioned between areas, populations within areas, and maternal lines. The composition of the root exudates was determined by metabolic profiling using ultra-high-performance liquid chromatography with time-of-flight mass spectrometry, and the relationships between the metabolic profiles and the effects observed in the bioassays were tested using orthogonal partial least-squares analysis. KEY RESULTS: Variance partitioning indicated that the highest variance in phytotoxic effects was within populations. The inhibition of germination observed in the bioassay for the co-occurring native species P. lanceolata could be predicted by the metabolic profiles of the root exudates of particular maternal lines. Fifteen compounds associated with this inhibition were tentatively identified. CONCLUSIONS: The results present strong evidence that intraspecific variability needs to be considered in research on allelopathy, and suggest that metabolic profiling provides an efficient tool for studying chemically mediated plant-plant interactions whenever unknown metabolites are involved.

• MeSH
• alelopatie * MeSH
• Arabidopsis účinky léků   MeSH
• bolševník chemie   genetika   metabolismus   MeSH
• druhová specificita MeSH
• ekosystém MeSH
• klíčení účinky léků   MeSH
• kořeny rostlin chemie   genetika   metabolismus   MeSH
• metabolom * MeSH
• Plantago účinky léků   MeSH
• rostlinné exsudáty chemie   izolace a purifikace   metabolismus   MeSH
• zavlečené druhy MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH