- MeSH
- bilirubin * izolace a purifikace krev metabolismus MeSH
- biopsie metody MeSH
- choláty izolace a purifikace metabolismus škodlivé účinky MeSH
- cholestáza * klasifikace patofyziologie patologie MeSH
- diagnostické techniky gastrointestinální klasifikace trendy MeSH
- diagnostické techniky molekulární metody trendy MeSH
- histologické techniky metody trendy MeSH
- hyperbilirubinemie komplikace patofyziologie patologie MeSH
- imunohistochemie metody MeSH
- játra anatomie a histologie patofyziologie patologie MeSH
- klinické laboratorní techniky metody trendy MeSH
- laboratorní medicína metody MeSH
- lidé MeSH
- mezioborová komunikace MeSH
- statistika jako téma MeSH
- žluč * fyziologie metabolismus MeSH
- žlučové kyseliny a soli izolace a purifikace metabolismus škodlivé účinky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Various thioacridine derivatives are potential chemotherapeutics against various diseases which are intensively synthesized, characterized, and investigated by many research groups. Efficient, fast, and reliable separation and quantification methods for their analysis are still to be developed. MEKC and capillary LC (CLC) were applied for the separation and quantification of five highly hydrophobic, weakly basic, and structurally similar 9-(alkylthio)acridines. Since the common anionic and cationic surfactants failed to separate the strongly hydrophobic thioacridines by MEKC, sodium cholate was used in an alkaline BGE and successfully employed for their fast separation. In CLC, the weakly basic nature of the thioacridines necessitated use of LiChrosorb RP-select B sorbent as the stationary phase, which combined with a very simple mobile phase methanol/water yielded an efficient chromatographic separation system. Both, the MEKC and CLC optimized separation methods were then applied to quantify the thioacridines within a concentration range of 1.0 x 10(-5)-1.0 x 10(-3) mol/L and the obtained experimental results were critically compared. In practical terms, the MEKC analytical method can quantify the analytes much faster but with a lower reliability while the CLC method performs slower analysis with a higher repeatability of the experimental results.
- MeSH
- apolipoproteiny A genetika MeSH
- cholát sodný aplikace a dávkování MeSH
- cholesterol aplikace a dávkování krev MeSH
- dieta metody MeSH
- finanční podpora výzkumu jako téma MeSH
- játra cytologie patologie MeSH
- lipoproteiny krev MeSH
- myši knockoutované krev metabolismus MeSH
- myši MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- MeSH
- apolipoproteiny E nedostatek MeSH
- choláty aplikace a dávkování škodlivé účinky MeSH
- cholesterol aplikace a dávkování krev MeSH
- dieta MeSH
- hypercholesterolemie diagnóza etiologie MeSH
- játra patologie účinky léků MeSH
- krysa rodu rattus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- choláty fyziologie MeSH
- H-2 antigeny fyziologie MeSH
- histokompatibilita - antigeny chemie imunologie MeSH
- myši MeSH
- Check Tag
- myši MeSH
