Background: A histopathological examination was conducted on vein samples from six patients who had undergone great saphenous vein sealing with Histoacryl and Lipiodol to treat chronic venous insufficiency of the superficial venous system. Patients and methods: In each patient a sample of the complete vein including the surrounding tissue was obtained through a minor incision under perioperative sonographic monitoring. Each patient had signed an informed consent form. Samples were taken at the following intervals: six days, six weeks, six months, one year, two years, and three years after treatment. Results: N-butyl-2-cyanoacrylate with Lipiodol induces a thrombotic reaction in the acute phase and endothelium is destroyed but no significant inflammation or substantial vascular wall damage is present. In the subsequent period, a foreign body giant cell reaction emerges accompanied by only moderate chronic inflammation that does not extend to the vascular wall or the surrounding tissue. The thrombus is organized with minimal recanalization. The foreign material is gradually degraded, and it is no longer detectable after three years. The vessel wall showed slight sclerotization. Conclusions: N-butyl-2-cyanoacrylate that has been applied gradually degrades over the course of three years accompanied by a giant cell reaction, mild chronic inflammation and cicatrices, but there is minimal recanalization of the obturated section.

• MeSH
• enbukrylát MeSH
• lidé MeSH
• varixy * MeSH
• vena saphena * MeSH
• výsledek terapie MeSH
• žilní insuficience * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND: Treating great and small saphenous vein trunk insufficiency with cyanoacrylate glue is the least taxing treatment method of all available techniques. Due to long-term unavailability of commercial kits with n-butyl-2-cyanoacrylate (histoacryl) in the Czech Republic, we used a modified technique. PATIENTS AND METHODS: Fifty-six limbs in 49 patients suffering from great saphenous vein or small saphenous vein insufficiency in combination with symptomatic chronic venous insufficiency and complicating comorbidities were treated with a modified endovascular cyanoacrylate glue application technique. RESULTS: The immediate success rate of the treatment was 98 %. In follow-up intervals of six weeks, six months, one year, and two years, the anatomical success rates of embolization (recanalization of no more than 5 cm of the junction) were 98, 96, 94, and 94 %, respectively. At identical intervals the venous insufficiency was scored according to the Aberdeen Varicose Vein Questionnaire and the American Venous Clinical Severity Score. In both cases, improvement was demonstrated over the two-year follow-up, with a 0.5 % significance level. Specific clinical signs of venous insufficiency were also evaluated, such as pain, oedema, clearance of varicose veins, and healing of venous ulceration. One severe complication - a pulmonary embolism - was reported, without consequences. CONCLUSIONS: We demonstrated that treating insufficient saphenous veins with modified histoacryl application brought a relief from symptoms of venous insufficiency and that the efficiency of this technique is comparable to commonly used methods.

• MeSH
• časové faktory MeSH
• chronická nemoc MeSH
• dospělí MeSH
• enbukrylát aplikace a dávkování   škodlivé účinky   MeSH
• endovaskulární výkony * škodlivé účinky   MeSH
• Kaplanův-Meierův odhad MeSH
• lidé středního věku MeSH
• lidé MeSH
• prospektivní studie MeSH
• rizikové faktory MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• stupeň závažnosti nemoci MeSH
• terapeutická embolizace * škodlivé účinky   MeSH
• varixy diagnostické zobrazování   chirurgie   terapie   MeSH
• vena saphena * diagnostické zobrazování   chirurgie   MeSH
• výsledek terapie MeSH
• žilní insuficience diagnostické zobrazování   chirurgie   terapie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

To determine whether PHEMA [poly(2-hydroxyethylmethacrylate)] is suitable for portal vein embolization in patients scheduled to right hepatectomy and whether it is as effective as the currently used agent (a histoacryl/lipiodol mixture). Two groups of nine patients each scheduled for extended right hepatectomy for primary or secondary hepatic tumor, had right portal vein embolization in an effort to induce future liver remnant (FLR) hypertrophy. One group had embolization with PHEMA, the other one with the histoacryl/lipiodol mixture. In all patients, embolization was performed using the right retrograde transhepatic access. Embolization was technically successful in all 18 patients, with no complication related to the embolization agent. Eight patients of either group developed FLR hypertrophy allowing extended right hepatectomy. Likewise, one patient in each group had recanalization of a portal vein branch. Histology showed that both embolization agents reach the periphery of portal vein branches, with PHEMA penetrating somewhat deeper into the periphery. PHEMA has been shown to be an agent suitable for embolization in the portal venous system comparable with existing embolization agent (histoacryl/lipiodol mixture).

• MeSH
• enbukrylát farmakologie   terapeutické užití   MeSH
• ethjodizovaný olej farmakologie   terapeutické užití   MeSH
• hepatektomie metody   MeSH
• játra účinky léků   MeSH
• lidé středního věku MeSH
• lidé MeSH
• polyhydroxyethylmethakrylát farmakologie   terapeutické užití   MeSH
• senioři MeSH
• terapeutická embolizace metody   MeSH
• vena portae účinky léků   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky kontrolované MeSH
• srovnávací studie MeSH

The Enhanced Permeability and Retention (EPR) effect is extensively used in drug delivery research. Taking into account that EPR is a highly variable phenomenon, we have here set out to evaluate if contrast-enhanced functional ultrasound (ceUS) imaging can be employed to characterize EPR-mediated passive drug targeting to tumors. Using standard fluorescence molecular tomography (FMT) and two different protocols for hybrid computed tomography-fluorescence molecular tomography (CT-FMT), the tumor accumulation of a ~10 nm-sized near-infrared-fluorophore-labeled polymeric drug carrier (pHPMA-Dy750) was evaluated in CT26 tumor-bearing mice. In the same set of animals, two different ceUS techniques (2D MIOT and 3D B-mode imaging) were employed to assess tumor vascularization. Subsequently, the degree of tumor vascularization was correlated with the degree of EPR-mediated drug targeting. Depending on the optical imaging protocol used, the tumor accumulation of the polymeric drug carrier ranged from 5 to 12% of the injected dose. The degree of tumor vascularization, determined using ceUS, varied from 4 to 11%. For both hybrid CT-FMT protocols, a good correlation between the degree of tumor vascularization and the degree of tumor accumulation was observed, within the case of reconstructed CT-FMT, correlation coefficients of ~0.8 and p-values of <0.02. These findings indicate that ceUS can be used to characterize and predict EPR, and potentially also to pre-select patients likely to respond to passively tumor-targeted nanomedicine treatments.

• MeSH
• akrylamidy aplikace a dávkování   MeSH
• enbukrylát MeSH
• kontrastní látky aplikace a dávkování   MeSH
• krevní objem MeSH
• mikrobubliny MeSH
• myši nahé MeSH
• nádorové buněčné linie MeSH
• nádory krevní zásobení   metabolismus   patofyziologie   ultrasonografie   MeSH
• permeabilita MeSH
• regionální krevní průtok MeSH
• systémy cílené aplikace léků * MeSH
• tomografie metody   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• anestezie metody   využití   MeSH
• antibakteriální látky terapeutické užití   MeSH
• enbukrylát terapeutické užití   MeSH
• hepatektomie metody   využití   MeSH
• klinické laboratorní techniky využití   MeSH
• kontrastní látky diagnostické užití   MeSH
• lidé MeSH
• mezioborová komunikace MeSH
• perioperační péče MeSH
• počítačová rentgenová tomografie metody   využití   MeSH
• pooperační komplikace MeSH
• předoperační péče metody   využití   MeSH
• selhání jater MeSH
• terapeutická embolizace kontraindikace   metody   normy   MeSH
• ultrasonografie metody   využití   MeSH
• vena portae patologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• směrnice pro lékařskou praxi MeSH

Hemobilia is a rare phenomenon. In this case report we present an emergent transcatheter glue embolization (in which N-butyl cyanoacrylate is used as an embolizing agent) due to arteriobilary fistula occurred following the laparoscopic cholecystectomy in a 41-year-old woman.

• MeSH
• biliární píštěl etiologie   terapie   MeSH
• cévní píštěle etiologie   terapie   MeSH
• cholecystektomie laparoskopická škodlivé účinky   MeSH
• dospělí MeSH
• enbukrylát terapeutické užití   MeSH
• endovaskulární výkony MeSH
• hemobilie etiologie   MeSH
• krvácení etiologie   terapie   MeSH
• lidé MeSH
• terapeutická embolizace MeSH
• tkáňová adheziva terapeutické užití   MeSH
• žlučník krevní zásobení   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

The aim of the study is to determine and compare the pharmacokinetics and tissue distribution of Doxorubicin (Dox) delivered as solution or through nanoparticles after intravenous (i.v.) and intraperitoneal (i.p.) injection. Doxorubicin loaded poly(butyl cyanoacrylate) nanoparticles were synthesized by dispersion polymerization (DP) and emulsion polymerization (EP) techniques. The drug loaded DP and EP nanoparticles were administered by i.v. or i.p. routes and the respective pharmacokinetics and tissue distribution were determined. Both types of nanoparticles significantly enhanced the elimination half-life (T(1/2)), mean residence time (MRT) AUC(0-8), AUC(0-infinity) and AUMC(0-8) of Dox in blood after i.v. injection. Dox delivered through DP nanoparticles rapidly disappeared from blood and distributed to the organs of reticuloendothelial system (RES). But, the clearance of Dox delivered through EP nanoparticles from blood was slower than this of the DP nanoparticles and Dox solution. After i.p. injection, the Dox loaded into DP nanoparticles quickly appeared in blood and undergone rapid distribution to the organs of RES, while the Dox loaded into EP nanoparticles absorbed slowly into blood and remained in the circulation for longer time. The absorption into blood of Dox delivered through DP and EP nanoparticles after i.p. injection was relatively rapid and higher than Dox solution. The T(1/2), MRT, AUC(0-8), AUC(0-infinity) and AUMC(0-8) of Dox in blood were significantly higher and the clearance (Cl) was lower than for the Dox solution after i.p. injection. The tissue concentrations of Dox delivered through nanoparticles after i.p. injection were significantly lower than after i.v. injection. The bioavailability (F) of Dox was greatly enhanced by DP (approximately 1.9 fold) and EP nanoparticles (approximately 2.12 fold) compared to Dox solution after i.p. injection. EP nanoparticles significantly enhanced the bioavailability, MRT, T(1/2), AUC(0-8), AUC(0-infinity) and AUMC(0-8) of Dox than DP nanoparticles. This signifies the advantage of EP nanoparticles in increasing the elimination half-life of Dox both after i.v. and i.p. injection and enhanced bioavailability after i.p. injection, which is expected to improve the therapeutic efficacy of Dox and reduce the Dox-associated systemic toxicity. Importantly, both DP and EP nanoparticles greatly reduced the distribution of Dox to heart both after i.v. and i.p. injection, suggesting their potential in reducing Dox-associated cardiotoxicity.

• MeSH
• antibiotika antitumorózní aplikace a dávkování   farmakokinetika   MeSH
• doxorubicin aplikace a dávkování   farmakokinetika   MeSH
• enbukrylát MeSH
• injekce intraperitoneální MeSH
• injekce intravenózní MeSH
• krysa rodu rattus MeSH
• nanostruktury MeSH
• nosiče léků MeSH
• plocha pod křivkou MeSH
• tkáňová distribuce MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

Randomizovaná kontrolovaná monocentrická studie preventivní endoskopické sklerotizace jícnových varixů jako jeden z podkladů stanoviska IASL k preventivní skleroterapii.

• MeSH
• enbukrylát terapeutické užití   MeSH
• ezofageální a žaludeční varixy terapie   MeSH
• gastrointestinální endoskopie využití   metody   MeSH
• interpretace statistických dat MeSH
• miniinvazivní chirurgické výkony využití   metody   MeSH
• portální hypertenze patofyziologie   klasifikace   MeSH
• skleroterapie využití   metody   MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• gastroenterologie
• radiologie, nukleární medicína a zobrazovací metody
• chemie, klinická chemie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

• MeSH
• enbukrylát aplikace a dávkování   terapeutické užití   MeSH
• lidé MeSH
• nemoci cervix uteri diagnóza   chirurgie   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH