- Klíčová slova
- Priessnitz walking,
- MeSH
- hydroterapie metody MeSH
- kryoterapie metody MeSH
- lidé MeSH
- nordic walking * MeSH
- Check Tag
- lidé MeSH
- MeSH
- aplikace inhalační MeSH
- balneologie * metody MeSH
- dechová cvičení metody MeSH
- dietoterapie metody MeSH
- dítě MeSH
- hydroterapie metody MeSH
- lázně MeSH
- lidé MeSH
- masáž metody MeSH
- plicní nemoci * rehabilitace terapie MeSH
- terapie bahnem metody MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- přehledy MeSH
- Klíčová slova
- Bělehrad,
- MeSH
- dějiny 20. století MeSH
- helioterapie MeSH
- hydroterapie MeSH
- indukovaná hypertermie MeSH
- lázně * dějiny MeSH
- lidé MeSH
- terapie bahnem MeSH
- Check Tag
- dějiny 20. století MeSH
- lidé MeSH
- Publikační typ
- historické články MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- balneologie * MeSH
- hydroterapie metody MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused considerable disruption worldwide. For efficient SARS-CoV-2 detection, new methods of rapid, non-invasive sampling are needed. This study aimed to investigate the stability of SARS-CoV-2 in a novel medium for gargle-lavage (GL) self-sampling and to compare the performance of SARS-CoV-2 detection in paired self-collected GL and clinician-obtained nasopharyngeal swab (NPS) samples. The stability study for SARS-CoV-2 preservation in a novel medium was performed over 14 days (4 °C, 24-27 °C, and 37 °C). In total, 494 paired GL and NPS samples were obtained at the University Hospital in Olomouc in April 2021. SARS-CoV-2 detection in paired samples was performed with a SARS-CoV-2 Nucleic Acid Detection Kit (Zybio, Chongqing Municipality, Chongqing, China), an Elecsys® SARS-CoV-2 Antigen assay (Roche Diagnostics, Mannheim, Germany), and a SARS-CoV-2 Antigen ELISA (EUROIMMUN, Lübeck, Germany). The stability study demonstrated excellent SARS-CoV-2 preservation in the novel medium for 14 days. SARS-CoV-2 was detected in 55.7% of NPS samples and 55.7% of GL samples using rRT-PCR, with an overall agreement of 91.9%. The positive percent agreement (PPA) of the rRT-PCR in the GL samples was 92.7%, and the negative percent agreement (NPA) was 90.9%, compared with the NPS samples. The PPA of the rRT-PCR in the NPS and GL samples was 93.2% when all positive tests were used as the reference standard. Both antigen detection assays showed poor sensitivity compared to rRT-PCR (33.2% and 36.0%). rRT-PCR SARS-CoV-2 detection in self-collected GL samples had a similar PPA and NPA to that of NPSs. GL self-sampling offers a suitable and more comfortable alternative for SARS-CoV-2 detection.
- MeSH
- lidé MeSH
- odporový trénink * MeSH
- vodní terapie * metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- novinové články MeSH
