Neuropeptides are involved in numerous brain activities being responsible for a wide spectrum of higher mental functions. The purpose of this concise, structural and qualitative investigation was to map the possible immunoreactivity of the novel regulatory peptides: spexin (SPX) and nesfatin-1 within the human claustrum. SPX is a newly identified peptide, a natural ligand for the galanin receptors (GALR) 2/3, with no molecular structure similarities to currently known regulatory factors. SPX seems to have multiple physiological functions, with an involvement in reproduction and food-intake regulation recently revealed in animal studies. Nesfatin-1, a second pleiotropic neuropeptide, which is a derivative of the nucleobindin-2 (NUCB-2) protein, is characterized by a wide distribution in the brain. Nesfatin-1 is a substance with a strong anorexigenic effect, playing an important role in the neuronal circuits of the hypothalamus that regulate food intake and energy homeostasis. On the other hand, nesfatin-1 may be involved in several important brain functions such as sleep, reproductive behaviour, cognitive processes, stress responses and anxiety. For the first time we detected and described a population of nesfatin-1 and SPX expressing neurons in the human claustrum using immunohistochemical and fluorescent methods. The study presents the novel identification of SPX and nesfatin-1 immunopositive neurons in the human claustrum and their assemblies show similar patterns of distribution in the whole structure.

• MeSH
• Claustrum * metabolism   MeSH
• Humans MeSH
• Neurons metabolism   MeSH
• Neuropeptides * metabolism   MeSH
• Nucleobindins metabolism   MeSH
• Nerve Tissue Proteins metabolism   MeSH
• Calcium-Binding Proteins metabolism   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH

The aim of the present study was to analyze the location of degenerating neurons in the dorsal (insular) claustrum (DCL, VCL) and the dorsal, intermediate and ventral endopiriform nucleus (DEn, IEn, VEn) in rat pups following lithium-pilocarpine status epilepticus (SE) induced at postnatal days [P]12, 15, 18, 21 and 25. The presence of Fluoro-Jade B-positive neurons was evaluated at 4, 12, 24, 48 h and 1 week later. A small number of degenerated neurons was observed in the CL, as well as in the DEn at P12 and P15. The number of degenerated neurons was increased in the CL as well as in the DEn at P18 and above and was highest at longer survival intervals. The CL at P15 and 18 contained a small or moderate number of degenerated neurons mainly close to the medial and dorsal margins also designated as DCl ("shell") while isolated degenerated neurons were distributed in the VCl ("core"). In P21 and 25, a larger number of degenerated neurons occurred in both subdivisions of the dorsal claustrum. The majority of degenerated neurons in the endopiriform nucleus were found in the intermediate and caudal third of the DEn. A small number of degenerated neurons was dispersed in the whole extent of the DEn with prevalence to its medial margin. Our results indicate that degenerated neurons in the claustrum CL and endopiriform nucleus are distributed mainly in subdivisions originating from the ventral pallium; their distribution correlates with chemoarchitectonics of both nuclei and with their intrinsic and extrinsic connections.

• MeSH
• Claustrum * MeSH
• Rats MeSH
• Cerebral Cortex MeSH
• Neurons MeSH
• Status Epilepticus * MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH

Footshock is frequently used as an unconditioned stimulus in fear conditioning behavior studies. The medial entorhinal cortex (MEC) contributes to fear learning and receives neuronal inputs from the claustrum. However, whether footshocks can induce a neuronal response in claustrum-MEC (CLA-MEC) projection remains unknown. Here, we combined fiber-based Ca2+ recordings with a retrograde AAV labeling method to investigate neuronal responses of MEC-projecting claustral neurons to footshock stimulation in freely moving mice. We achieved successful Ca2+ recordings in both anesthetized and freely exploring mice. We found that footshock stimulation reliably induced neuronal responses to MEC-projecting claustral neurons. Therefore, the footshock-induced response detected in the CLA-MEC projection suggests its potential role in fear processin.

• MeSH
• Entorhinal Cortex * physiology   MeSH
• Claustrum * MeSH
• Mice MeSH
• Neurons physiology   MeSH
• Fear MeSH
• Calcium MeSH
• Animals MeSH
• Check Tag
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH