Monoclonal antibodies IAH-CC51, BB6-11C9.6 and B-Ly4 are routinely used to detect CD21 orthologue on the surface of porcine B lymphocytes. Cross-reactive studies show that IAH-CC51 and B-Ly4 recognize only a portion of B cells that are positive for pan-specific BB6-11C9.6. This indicates that CD21 is always present on all mature B cells but can be expressed in at least two differential forms, and these were assigned as CD21(a) and CD21(b). We used IAH-CC51 together with anti-CD2 to define four subpopulations of B cells. Ontogenetic and in vitro culture studies, analysis of cell size, expression of CD11b and class-switched phenotype together with measurement of proliferation and cell death, revealed that these subsets represent distinct populations. Phenotypic and functional features collectively suggest that CD21(b+) B cells are less mature than CD21(b-). The present work is the first to show that distinct subsets of mature B cells can express differential forms of CD21.

• MeSH
• apoptóza MeSH
• epitopy imunologie   MeSH
• kultivované buňky MeSH
• monoklonální protilátky imunologie   MeSH
• podskupiny B-lymfocytů klasifikace   imunologie   MeSH
• prasata imunologie   MeSH
• receptory komplementu 3d imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The CD8+ natural killer (NK) subpopulation has recently been identified as a fast and reliable biodosimetric indicator within human peripheral blood mononuclear cells (PBMC) in vitro. In irradiated and subsequently cultivated PBMC, a decrease of the relative number of intact CD3-CD8+ lymphocytes 16 and 48 h after treatment has allowed for estimating the received dose in the range of 0 - 10 Gy and lethal/sublethal dose discrimination, respectively. Here we show that suitable biodosimeters can also be found in the peripheral blood B-cell compartment. Multiparameter flow cytometric analysis of irradiated and subsequently cultivated human PBMC revealed that both the CD27+ and CD21- B-cell subpopulations can be used as biodosimeters and the CD19+CD27+ lymphocytes have proved useful for retrospective determination of the received dose in the range of 0 - 6 Gy. In addition, several CD19+ lymphocyte subsets characterized by co-expression of CD21, CD27 and CD38 have been shown to bear biodosimetric potential, too. However, when important parameters like the original size within the CD19+ compartment, its radiation-induced changes and data variation had been taken into account, the CD27+ subpopulation proved superior to the other B-cell subpopulations and subsets. It appears that, in the dose range of 0 - 6 Gy, the relative decrease of CD27+ B lymphocytes provides more sensitive and reliable data than that of CD8+ NK-cells due mainly to lower data variation. In contrast to CD27+ B-cells, the proportions of CD27+ subpopulations of T-cells were not affected by irradiation. We have also proposed a simple experimental protocol based on full blood cultivation and three-color CD27/CD3/CD19 immunophenotyping as a time-saving and inexpensive approach for practical biodosimetric evaluations on simple, three-to-four color flow cytometers.

• Klíčová slova
• B-cells, CD27, Biodosimetric marker, ?-irradiation,
• MeSH
• annexin A5 metabolismus   MeSH
• antigeny CD27 fyziologie   MeSH
• antigeny CD38 fyziologie   MeSH
• B-lymfocyty fyziologie   účinky záření   MeSH
• biologické markery MeSH
• fenotyp MeSH
• financování organizované MeSH
• fosfatidylseriny metabolismus   MeSH
• lidé MeSH
• monocyty fyziologie   MeSH
• podskupiny lymfocytů fyziologie   MeSH
• průtoková cytometrie MeSH
• receptory buněčného povrchu metabolismus   MeSH
• receptory komplementu 3d metabolismus   MeSH
• separace buněk MeSH
• vztah dávky záření a odpovědi MeSH
• záření gama MeSH
• Check Tag
• lidé MeSH