- Keywords
- senzory v obvazech, pyranin,
- MeSH
- Arylsulfonates MeSH
- Biosensing Techniques * instrumentation utilization MeSH
- Chronic Disease MeSH
- Fluorescence MeSH
- Fluorescent Dyes MeSH
- Wound Healing MeSH
- Hydrogen-Ion Concentration MeSH
- Humans MeSH
- Occlusive Dressings * MeSH
- Wounds and Injuries * nursing therapy MeSH
- Check Tag
- Humans MeSH
- MeSH
- Adenosine pharmacology therapeutic use MeSH
- Purinergic P2Y Receptor Antagonists pharmacology therapeutic use MeSH
- Biosensing Techniques methods utilization MeSH
- Hypertension * drug therapy therapy MeSH
- ST Elevation Myocardial Infarction * drug therapy MeSH
- Cardiology * MeSH
- Congresses as Topic MeSH
- Humans MeSH
- Human Embryonic Stem Cells MeSH
- Ultrasonic Therapy methods utilization MeSH
- Check Tag
- Humans MeSH
- Publication type
- News MeSH
There is a rapidly growing interest in low-cost, fast and sensitive biosensors. In particular, direct determination of important metabolites, serving as biomarkers of various pathological states can significantly enhance the treatment successes. In our study, we introduce a technical concept of a 3D printed biosensor, which employs polydimethylsiloxane chip with volume of 50 µL as an inert and optically clear reservoir for recognition element and fluorescence detection. By using a 3D printing technology, low production cost and high crafting reproducibility were achieved. Due to a presence of controlled electromagnet, the biosensor can be utilized for a broad spectrum of applications, based on paramagnetic nano- or microscaled materials.
Glycan biochips and biosensors are potentially important tools for detection of glycan – protein interactions. Among several applications they can be used for rapid and precise diagnosis of various diseases and infections. Two major detection techniques available for construction of glycan biochips and biosensors – fluorescent labelled and label-free methods are discussed.
- MeSH
- Bacterial Infections * diagnosis MeSH
- Biosensing Techniques * methods instrumentation utilization MeSH
- Chemistry Techniques, Analytical MeSH
- Diagnostic Imaging methods utilization MeSH
- DNA Probes * MeSH
- Fluoroimmunoassay methods utilization MeSH
- Humans MeSH
- Optical Imaging methods utilization MeSH
- Polysaccharides analysis chemistry MeSH
- Surface Plasmon Resonance methods instrumentation utilization MeSH
- Proteins chemistry MeSH
- Spectrum Analysis methods utilization MeSH
- Virus Diseases * diagnosis MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il., tab. ; 31 cm
Po zavedení nových citlivých metod (ELISA a senzory s povrchovými plazmony) budou v mozkomíšním moku dementních a nedementních lidí testovány hladiny dvou komplexů peptidu amyloidu beta (s mitochondriálním enzymem 17beta-hydroxysteroidovou dehydrogenázoutypu 10 a proteinem tau). Pomocí kitů budou dále stanoveny hladiny tripletu (amyloid beta 1-42, celkový tau a fosforylovaný tau).; After developments of new highly sensitive methods (via ELISA and surface plasmon resonance biosensors) , we will evaluate the levels of two complexes of amyloid beta peptides with either mitochondrial enzyme 17beta-hydroxysteroid dehydrogenase type 10 or protein tau in cerebrospinal fluids of demented and nondemented people. The results will be compared with those of triplet (amyloid beta peptide 1-42, total tau and phospho-tau levels by means of kits).
- MeSH
- 17-Hydroxysteroid Dehydrogenases analysis MeSH
- Alzheimer Disease diagnosis MeSH
- Amyloid beta-Peptides analysis MeSH
- Biomarkers analysis MeSH
- Biosensing Techniques methods instrumentation utilization MeSH
- Early Diagnosis MeSH
- Economics, Medical MeSH
- Enzyme-Linked Immunosorbent Assay methods utilization MeSH
- Interdisciplinary Communication MeSH
- Research MeSH
- Conspectus
- Biochemie. Molekulární biologie. Biofyzika
- NML Fields
- neurologie
- biologie
- NML Publication type
- závěrečné zprávy o řešení grantu IGA MZ ČR
x
- Keywords
- piezoelectric based biosenzor,
- MeSH
- Biosensing Techniques * methods instrumentation utilization MeSH
- DNA MeSH
- Electrochemical Techniques * methods instrumentation utilization MeSH
- Enzymes analysis MeSH
- Luminescent Measurements methods MeSH
- Transducers MeSH
- Optical Fibers MeSH
- Surface Plasmon Resonance MeSH
- Antibodies analysis MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- MeSH
- Biosensing Techniques * history methods instrumentation trends utilization MeSH
- Diabetes Mellitus diagnosis blood MeSH
- Enzymes biosynthesis MeSH
- Glucose analysis chemistry MeSH
- Blood Glucose MeSH
- Humans MeSH
- Nanoparticles utilization MeSH
- Potentiometry instrumentation MeSH
- Blood Glucose Self-Monitoring * instrumentation trends MeSH
- Check Tag
- Humans MeSH
The cholinesterase biosensors are analytical devices suitable for assay of cholinesterase inhibitors in the course of suppression of the biorecognition element activity. Both acetylcholinesterase and butyrylcholinesterase are used for their assay. The biosensors can be used for assay of organophosphorus and carbamate pesticides, nerve agents, drugs in Alzheimer´s disease and myasthenia treatment. The review summarizes the knowledge of cholinesterases, their immobilization and inhibitors as well as shows examples of their activities.
- MeSH
- Acetylcholinesterase MeSH
- Alzheimer Disease MeSH
- Biosensing Techniques * methods utilization MeSH
- Butyrylcholinesterase MeSH
- Cholinesterase Inhibitors * MeSH
- Dichlorvos * analysis standards MeSH
- Carbamates * pharmacology MeSH
- Humans MeSH
- Organofluorophosphonates * pharmacology MeSH
- Cholinesterase Reactivators analysis MeSH
- Sarin pharmacology MeSH
- Check Tag
- Humans MeSH
