Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans , a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with α- or β-glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (∼58 mol %), mannose (∼22 mol %), and galactose (∼18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue α-D-Glcp-(1→2)-β-D-Galf-(1→6)-α-D-Manp-(1→3)-L-Ser. Terminal α-D-Glcp and (1→6)-β-D-glucan were also detected. The obtained results strongly support the conclusion that these carbohydrates induce defense response in B. napus plants.
- MeSH
- Ascomycota chemistry growth & development immunology MeSH
- Aspergillus niger enzymology MeSH
- Brassica napus drug effects immunology metabolism microbiology MeSH
- Cell Wall chemistry MeSH
- Cell Extracts chemistry isolation & purification pharmacology MeSH
- Down-Regulation MeSH
- Fungal Proteins analysis chemistry metabolism MeSH
- Glycoside Hydrolases biosynthesis genetics metabolism MeSH
- Glycosides analysis chemistry metabolism MeSH
- Hydrolysis MeSH
- Mycelium chemistry growth & development immunology MeSH
- Disease Resistance drug effects MeSH
- Oligosaccharides analysis chemistry metabolism MeSH
- Peptide Fragments analysis chemistry metabolism MeSH
- Hydrogen Peroxide metabolism MeSH
- Fungicides, Industrial chemistry isolation & purification pharmacology MeSH
- Plant Proteins biosynthesis genetics metabolism MeSH
- Seedlings drug effects immunology metabolism microbiology MeSH
- Up-Regulation drug effects MeSH
- Chemistry, Agricultural methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with jasmonic acid (JA) and pectin as elicitors to evaluate their effect on metabolism from two cell lines using NMR spectroscopy and multivariate data analysis. According to principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA), the chloroform extract of the pectin-treated cultures were more different than control and JA-treated cultures; but in the methanol/water extract the metabolome of the JA-treated cells showed clear differences with control and pectin-treated cultures. Tyrosol, an antioxidant metabolite, was detected in cannabis cell cultures. The tyrosol content increased after eliciting with JA.
- MeSH
- Cell Extracts chemistry isolation & purification MeSH
- Cannabis chemistry metabolism MeSH
- Cyclopentanes metabolism MeSH
- Phenylethyl Alcohol analogs & derivatives analysis MeSH
- Cells, Cultured MeSH
- Magnetic Resonance Spectroscopy MeSH
- Metabolome MeSH
- Oxylipins metabolism MeSH
- Pectins metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Arthritis, Experimental metabolism pathology therapy MeSH
- Biopterins urine MeSH
- Cell Extracts isolation & purification therapeutic use MeSH
- Rats MeSH
- Hyaluronic Acid blood MeSH
- Leukocytes chemistry MeSH
- Serum Albumin analysis MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Comparative Study MeSH
