Photosynthetic energy conversion and the resulting photoautotrophic growth of green algae can only occur in daylight, but DNA replication, nuclear and cellular divisions occur often during the night. With such a light/dark regime, an algal culture becomes synchronized. In this study, using synchronized cultures of the green alga Desmodesmus quadricauda, the dynamics of starch, lipid, polyphosphate, and guanine pools were investigated during the cell cycle by two independent methodologies; conventional biochemical analyzes of cell suspensions and confocal Raman microscopy of single algal cells. Raman microscopy reports not only on mean concentrations, but also on the distribution of pools within cells. This is more sensitive in detecting lipids than biochemical analysis, but both methods-as well as conventional fluorescence microscopy-were comparable in detecting polyphosphates. Discrepancies in the detection of starch by Raman microscopy are discussed. The power of Raman microscopy was proven to be particularly valuable in the detection of guanine, which was traceable by its unique vibrational signature. Guanine microcrystals occurred specifically at around the time of DNA replication and prior to nuclear division. Interestingly, guanine crystals co-localized with polyphosphates in the vicinity of nuclei around the time of nuclear division.
- MeSH
- Cell Wall chemistry MeSH
- Cell Cycle * MeSH
- Time Factors MeSH
- Chlorophyta cytology growth & development MeSH
- Guanine analysis MeSH
- Lipid Droplets metabolism MeSH
- Lipids analysis MeSH
- Microscopy * MeSH
- Polyphosphates analysis MeSH
- Spectrum Analysis, Raman * MeSH
- Starch analysis MeSH
- Cell Size MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- MeSH
- Food Safety MeSH
- Flavoring Agents MeSH
- Sodium Nitrite analysis standards MeSH
- Food, Genetically Modified MeSH
- Food Quality MeSH
- Meat Products analysis standards MeSH
- Food Labeling MeSH
- Polyphosphates analysis standards MeSH
- Food Coloring Agents MeSH
- Food Technology methods standards MeSH
- Food Preservatives analysis standards MeSH
- Food Additives * MeSH
