• MeSH
• aminoglykosidy terapeutické užití   toxicita   MeSH
• antibakteriální látky terapeutické užití   MeSH
• antibiotická rezistence účinky léků   MeSH
• beta-laktamová antibiotika terapeutické užití   MeSH
• biologické markery analýza   krev   MeSH
• grampozitivní bakteriální infekce MeSH
• kongresy jako téma MeSH
• lidé MeSH
• methicilin rezistentní Staphylococcus aureus účinky léků   MeSH
• nežádoucí účinky léčiv MeSH
• rozvrh dávkování léků MeSH
• stafylokokové infekce * diagnóza   farmakoterapie   MeSH
• Staphylococcus aureus cytologie   účinky léků   MeSH
• synergismus léků MeSH
• způsoby aplikace léků MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• novinové články MeSH

Cadmium, as a hazardous pollutant commonly present in the living environment, represents an important risk to human health due to its undesirable effects (oxidative stress, changes in activities of many enzymes, interactions with biomolecules including DNA and RNA) and consequent potential risk, making its detection very important. New and unique technological and biotechnological approaches for solving this problems are intensely sought. In this study, we used the commonly occurring potential pathogenic microorganism Staphylococcus aureus for the determination of markers which could be used for sensing of cadmium(II) ions. We were focused on monitoring the effects of different cadmium(II) ion concentrations (0, 1.25, 2.5, 5, 10, 15, 25 and 50 μg mL(-1)) on the growth and energetic metabolism of Staphylococcus aureus. Highly significant changes have been detected in the metabolism of thiol compounds-specifically the protein metallothionein (0.79-26.82 mmol/mg of protein), the enzyme glutathione S-transferase (190-5,827 μmol/min/mg of protein), and sulfhydryl groups (9.6-274.3 μmol cysteine/mg of protein). The ratio of reduced and oxidized glutathione indicated marked oxidative stress. In addition, dramatic changes in urease activity, which is connected with resistance of bacteria, were determined. Further, the effects of cadmium(II) ions on the metabolic pathways of arginine, β-glucosidase, phosphatase, N-acetyl β-d-glucosamine, sucrose, trehalose, mannitol, maltose, lactose, fructose and total proteins were demonstrated. A metabolomic profile of Staphylococcus aureus under cadmium(II) ion treatment conditions was completed seeking data about the possibility of cadmium(II) ion accumulation in cells. The results demonstrate potential in the application of microorganisms as modern biosensor systems based on biological components.

• MeSH
• biosenzitivní techniky metody   MeSH
• disacharidy metabolismus   MeSH
• elektrochemické techniky MeSH
• fosfatasy metabolismus   MeSH
• glutathion metabolismus   MeSH
• glutathiondisulfid metabolismus   MeSH
• glutathiontransferasa metabolismus   MeSH
• hydrolasy metabolismus   MeSH
• kadmium analýza   metabolismus   farmakologie   MeSH
• látky znečišťující životní prostředí analýza   metabolismus   farmakologie   MeSH
• metabolismus účinky léků   MeSH
• metalothionein metabolismus   MeSH
• monosacharidy metabolismus   MeSH
• proliferace buněk účinky léků   MeSH
• proteiny metabolismus   MeSH
• Staphylococcus aureus cytologie   účinky léků   metabolismus   MeSH
• sulfhydrylové sloučeniny metabolismus   MeSH
• ureasa metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Medical-grade polyvinyl chloride was coated by polysaccharides through a novel physicochemical approach. An initial surface activation was performed foremost via diffuse coplanar surface barrier discharge plasma in air at ambient temperature and pressure. Then, radical graft copolymerization of acrylic acid through grafting-from pathway was directed to render a well-defined brush of high density, and finally a chitosan monolayer and chitosan/pectin alternating multilayer were bound onto the functionalized surfaces. Surface characteristics were systematically investigated using several probe techniques. In vitro bacterial adhesion and biofilm formation assays indicated that a single chitosan layer was incapable of hindering the adhesion of a Staphylococcus aureus bacterial strain, while up to 30% reduction was achieved by the chitosan/pectin layered assembly. On the other hand, chitosan and chitosan/pectin multilayer could retard Escherichia coli adhesion by 50% and 20%, respectively. Furthermore, plasma treated and graft copolymerized samples were also found effective to diminish the degree of adherence of Escherichia coli.

• MeSH
• absorpce účinky léků   MeSH
• bakteriální adheze účinky léků   MeSH
• biofilmy účinky léků   MeSH
• biokompatibilní potahované materiály farmakologie   MeSH
• Escherichia coli cytologie   účinky léků   fyziologie   MeSH
• fotoelektronová spektroskopie MeSH
• mikroskopie elektronová rastrovací MeSH
• molekulární modely MeSH
• polysacharidy farmakologie   MeSH
• polyvinylchlorid farmakologie   MeSH
• smáčivost účinky léků   MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• Staphylococcus aureus cytologie   účinky léků   fyziologie   MeSH
• voda MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH