The aqueous extract of Cichorium intybus (CIE) leaves have shown the properties of protecting against pancreatic β-cell damage by streptozotocin (STZ), but the molecular mechanisms of its protection are not completely elucidated yet. Our current study focuses on elucidating the mechanisms of these preventive effects of CIE in MIN6 cells and an in-vivo model of Wistar rats. CIE offers protection against STZ in MIN6 cells by reducing the pro-oxidants and increasing the activity of the antioxidant enzymes. In vitro results also indicated that CIE inhibited cytotoxicity, reduced Reactive oxygen species (ROS), maintained glucose-stimulated insulin secretion and reduced NF-κB p65 translocation into the nucleus. The group administered with a 250 mg/kg dose of CIE in vivo has shown an ability to maintain blood glucose level and also to preserve the number and morphology of pancreatic islets when compared to the diabetic group treated with STZ. Probably, active compounds like quercetin, rutin, and catechin present in CIE, preserve the integrity of pancreatic islets thereby protecting β-cells from the adverse effects of STZ.
- MeSH
- Adrenalectomy methods trends utilization MeSH
- Bevacizumab administration & dosage adverse effects therapeutic use MeSH
- Cetuximab administration & dosage adverse effects therapeutic use MeSH
- Cisplatin administration & dosage adverse effects therapeutic use MeSH
- Cushing Syndrome * diagnosis etiology therapy MeSH
- Doxorubicin administration & dosage adverse effects therapeutic use MeSH
- Etoposide administration & dosage adverse effects therapeutic use MeSH
- Drug Therapy * methods trends utilization MeSH
- Thoracic Surgery methods trends MeSH
- Hydrocortisone isolation & purification MeSH
- Pituitary ACTH Hypersecretion complications physiopathology prevention & control MeSH
- Humans MeSH
- Neoplasm Metastasis diagnosis prevention & control therapy MeSH
- Mitotane administration & dosage adverse effects therapeutic use MeSH
- Neoplasms, Hormone-Dependent enzymology therapy MeSH
- Radiosurgery * methods trends utilization MeSH
- Statistics as Topic MeSH
- Streptozocin administration & dosage adverse effects therapeutic use MeSH
- Check Tag
- Humans MeSH
The present study was carried out to investigate the hypoglycaemic effect of S-allyl cysteine (SAC), a garlic component, on some biochemical parameters of STZ induced diabetic rats. STZ induced diabetic rats were treated with SAC at two different doses (100 mg/kg b.w. and 150 mg/kg b.w.) for 45 days. Treatment with SAC significantly decreased the levels of blood glucose, glycosylated hemoglobin, blood urea, serum uric acid, serum creatinine, and diminished activities of pathophysiological enzymes such as aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP). The antihyperglycaemic nature of SAC is also evidenced from the improvement in the levels of plasma insulin and haemoglobin. Further, the results are comparable with glyclazide, an oral standard drug. A 150 mg/kg b.w. dose produced a better effect than a 100 mg dose. Thus, the present findings suggest that SAC may be considered as an effective therapeutic agent for the treatment of diabetes mellitus.
- MeSH
- Garlic chemistry MeSH
- Cysteine analogs & derivatives isolation & purification therapeutic use MeSH
- Diabetes Mellitus drug therapy prevention & control MeSH
- Enzyme-Linked Immunosorbent Assay methods utilization MeSH
- Animal Experimentation MeSH
- Hypoglycemic Agents metabolism therapeutic use MeSH
- Blood Glucose drug effects MeSH
- Rats, Wistar MeSH
- Plant Extracts isolation & purification metabolism therapeutic use MeSH
- Streptozocin administration & dosage adverse effects MeSH
- Body Weight MeSH
- Transaminases blood drug effects MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Tables MeSH
The present study investigated the effect of the aqueous extract of Helicteres isora L (Sterculiaceae) bark on oxidative stress in the heart of rats during diabetes. The aqueous extract of Helicteres isora bark (100 mg, 200 mg/kg body weight (b.w.)) was screened for its antioxidant effect in streptozotocin (STZ) induced diabetic rats. An appreciable decrease in peroxidation products, thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD), and hydroperoxides (HP) was observed in the heart tissues of Helicteres isora (HI) treated diabetic rats. The decreased activities of key antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-tranferase (GST) and glutathione (GSH) in diabetic rats were brought back to near normal range upon HI treatment. Tolbutamide was used as the standard reference drug. These results suggest that HI possesses promising antioxidative activity in STZ diabetic rats.
- MeSH
- Diabetes Mellitus, Experimental complications metabolism MeSH
- Catalase pharmacokinetics metabolism MeSH
- Lipid Peroxidation genetics radiation effects MeSH
- Rats, Wistar MeSH
- Reactive Oxygen Species metabolism adverse effects MeSH
- Plant Extracts pharmacokinetics metabolism MeSH
- Malvaceae metabolism drug effects MeSH
- Streptozocin metabolism adverse effects MeSH
- Superoxide Dismutase pharmacokinetics metabolism MeSH
- Tolbutamide pharmacokinetics metabolism MeSH
This study was designed to evaluate the effect of Terminalia chebula fruit extract on the levels of plasma and tissue glycoprotein components in streptozotocin-induced-diabetic rats. Oral administration of T. chebula fruit extract at a concentration of 200 mg/kg body weight for 30 days significantly reduced the levels of blood glucose, glycosylated hemoglobin, urea, and creatinine as well as fucose, hexose, hexosamine and sialic acid in the diabetic rats treated with the fruit extract. The observed decrease in the levels of plasma insulin and C-peptide in the diabetic rats was elevated to near normal by T. chebula fruit extract treatment. Histological observations made on the pancreatic tissue of control and experimental groups also revealed the beneficial effect of T. chebula fruit extract. The efficacy of the fruit extract was comparable with glibenclamide, a known hypoglycaemic drug.
- MeSH
- Medicine, Ayurvedic MeSH
- C-Peptide diagnostic use blood MeSH
- Diabetes Mellitus, Experimental complications metabolism MeSH
- Glyburide metabolism MeSH
- Glycoproteins metabolism adverse effects MeSH
- Insulin isolation & purification blood metabolism MeSH
- Rats, Wistar MeSH
- Streptozocin metabolism adverse effects MeSH
- Terminalia metabolism MeSH
The present investigation shows the antihyperglycaemic activity of aqueous extract of bark of Helicteres isora L. (100, 200mg/kg b.w/p.o) in streptozotocin (STZ) induced diabetic rats. Blood glucose levels, body weight, food and liquid intake were measured on every 5th day over a period of 14 days. A single injection of STZ at a dose of 60mg/kg b.w/i.p elevated the glucose levels >240mg/dl after 5 days. Administration of H.isora at a dose of 100, 200mg/kg/p.o resulted in a significant (p<0.05) reduction in blood glucose levels. Body weights were significantly (p<0.05) reduced in STZ-induced diabetic rats when compared to normal rats while the extract significantly (p<0.05) prevented a decrease in body weight in the H.isora treated animals. The study also evaluated the antioxidant potential of H.isora in STZ-induced diabetic rats. Decreased levels of thiobarbituric acid reactive substances (TBARS), increased levels of reduced glutathione (GSH) and the activities of superoxide dismutase (SOD) and catalase (CAT) resulted in the reduction of free radical formation in various tissues such as liver, kidney, and brain of the diabetic rats. Tolbutamide was used as a standard reference drug. The results clearly indicate that the aqueous extract of bark of Helicteres isora exhibits significant antihyperglycaemic and in vivo antioxidant activity in STZ-induced diabetic rats and the results were found to be dose dependent.
- MeSH
- Antioxidants therapeutic use MeSH
- Diabetes Mellitus, Experimental complications metabolism MeSH
- Hypoglycemic Agents therapeutic use MeSH
- Catalase pharmacokinetics metabolism MeSH
- Plant Bark MeSH
- Lipid Peroxidation genetics radiation effects MeSH
- Rats, Wistar metabolism MeSH
- Reactive Oxygen Species metabolism adverse effects MeSH
- Plant Extracts pharmacokinetics metabolism therapeutic use MeSH
- Malvaceae chemistry metabolism drug effects MeSH
- Streptozocin metabolism adverse effects MeSH
- Superoxide Dismutase pharmacokinetics metabolism MeSH
- Tolbutamide pharmacokinetics metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
