Soil organic matter is used to extrapolate the toxicity and bioavailability of organic pollutants between different soils. However, it has been shown that other factors such as microbial activity are crucial. The aim of this study was to investigate if sterilization can reduce differences in the fate and bioavailability of organic pollutants between different soils. Three natural soils with increasing total organic carbon (TOC) content were collected and three artificial soils were prepared to obtain similar TOCs. Soils were sterilized and spiked with (14)C-pyrene and (14)C-lindane. Total (14)C radioactivity, HPCD extractability, and bioaccumulation in Eisenia fetida were measured over 56 days. When compared to non-sterile soils, differences between the natural and artificial soils and the influence of soil-contaminant contact time were generally reduced in the sterile soils (especially with middle TOC). The results indicate the possibility of using sterile soils as "the worst case scenario" in soil ecotoxicity studies.
- MeSH
- hexachlorcyklohexan analýza metabolismus MeSH
- látky znečišťující půdu analýza metabolismus MeSH
- monitorování životního prostředí MeSH
- Oligochaeta metabolismus MeSH
- půda MeSH
- pyreny analýza metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: This pilot study was conducted to investigate biochemical effects of exposure to diesel engine exhaust at two mines. For this purpose, heme biosynthesis, and PP association with DNA (PP/DNA) in lymphocytes of miners exposed to diesel exhaust were determined. METHODS: The pilot study was carried out at a black coal mine in Czech Republic, and at an oil-shale mine in Estonia. The subjects were ten drivers from each mine, who were exposed to diesel exhaust (underground workers). Control groups consisted of ten maintenance workers from each mine (surface workers). The content of 1-nitropyrene (NP) in respirable dust was measured using GC-MS. The levels of PP, PP/DNA, heme, and activity of ferrochelatase (FC) were determined by spectrophotometry. RESULTS: The exposure to diesel exhaust evaluated as the level of NP associated to particulate matter in air. NP level was significantly higher in oil-shale mine compared with coal mine. The values of PP and PP/DNA in lymphocytes appeared to be significantly increased only in miners in the oil-shale mine. There was no difference in the levels of PP and PP/DNA and FC activity between surface workers and miners at the coal mine. The level of heme in lymphocytes of coal mine miners was significantly higher than in miners of the oil-shale mine. The activity of FC was significantly lower in underground workers compared to surface workers at this mine. High level of NP was accompanied by an increase of alterations in cells porphyrin metabolism in lymphocytes of miners. CONCLUSIONS: Alterations of porphyrin and heme metabolism in peripheral lymphocytes may serve as biomarker in assessment of exposure to diesel exhaust effects.
- MeSH
- DNA metabolismus MeSH
- dospělí MeSH
- ferrochelatasa metabolismus MeSH
- hem metabolismus MeSH
- hornictví * MeSH
- inhalační expozice analýza MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymfocyty účinky léků metabolismus MeSH
- mutageny metabolismus MeSH
- pilotní projekty MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- prach analýza MeSH
- pracovní expozice analýza MeSH
- protoporfyriny metabolismus MeSH
- pyreny metabolismus MeSH
- spektrofotometrie MeSH
- studie případů a kontrol MeSH
- výfukové emise vozidel analýza MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Estonsko MeSH
- MeSH
- chryseny metabolismus moč MeSH
- feces chemie MeSH
- fenantreny metabolismus moč MeSH
- karcinogeny MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- polycyklické aromatické uhlovodíky metabolismus MeSH
- pracovní expozice MeSH
- pyreny metabolismus moč MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
