Cadmium is a heavy metal abundant in the environment that can induce endocrine disorder and toxicity in aquatic organisms at low levels. However, its effects on the thyroid system in fish are still unclear. In this study, the thyroid hormone (TH) levels and the expression profiles of genes related to hypothalamic- pituitary-thyroid (HPT) axis, including corticotropin-releasing hormone (crh), thyroid stimulating hormone beta (tshβ), solute carrier family 5 (sodium iodide symporter) member 5 (slc5a5), thyroglobulin (tg), thyroid hormone receptor alpha (trα) and thyroid hormone receptor beta (trβ), were determined in whole body of Chinese rare minnow (Gobiocypris rarus) larvae after exposure to different levels of Cd(2+) (0, 0.5 and 2.5mg/L) for 4days. And the 96-h lethal concentration of Cd(2+) on rare minnow larvae was determined as 2.59mg/L. The results showed that crh, slc5a5, tg and tshβ mRNA levels were significantly up-regulated in the larvae, but the gene expression of trα and trβ was down-regulated in a concentration-dependent manner. Besides, the THs levels decreased in the whole-body of fish, especially the thyroxine (T4) level. The above results indicated that Cd(2+) could alter gene expression in the HPT axis that might subsequently contribute to thyroid disruption.

• MeSH
• chemické látky znečišťující vodu otrava   MeSH
• down regulace účinky léků   genetika   MeSH
• exprese genu účinky léků   MeSH
• hormony štítné žlázy metabolismus   MeSH
• kadmium škodlivé účinky   MeSH
• larva účinky léků   genetika   metabolismus   MeSH
• messenger RNA genetika   MeSH
• otrava kadmiem * MeSH
• ryby metabolismus   MeSH
• štítná žláza účinky léků   metabolismus   MeSH
• systém hypotalamus-hypofýza účinky léků   metabolismus   MeSH
• thyroxin metabolismus   MeSH
• upregulace účinky léků   genetika   MeSH
• vystavení vlivu životního prostředí MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Mercury is a prominent environmental contaminant that causes endocrine disorder to human and other organisms. But little is known about the response of the thyroid functions and hypothalamic-pituitary-thyroid (HPT) axis to mercury in teleosts and the few studies that are available have not yielded consistent results. In this study, expression profiles of corticotropin-releasing hormone (crh), thyroid stimulating hormone beta (tshβ), solute carrier family 5 (sodium iodide symporter) member 5 (slc5a5), thyroglobulin (tg), thyroid hormone receptor alpha (trα) and thyroid hormone receptor beta (trβ) genes were determined in whole-body of Chinese rare minnow (Gobiocypris rarus) larvae after exposure to different levels of Hg(2+) (0, 0.1 and 0.3 mg/l) for 4 days, as well as the thyroid hormones (THs) levels. Moreover, the 96-h lethal concentration of Hg(2+) on rare minnow larvae was determined as 0.32 mg/l. The results showed that crh, tg, trα and trβ mRNA levels were significantly up-regulated in the larvae, but the gene expression of tshβ and slc5a5 was not significantly changed in our study. Besides, the THs levels increased in the whole-body of fish, especially the thyroxine (T4) level. The above results indicated that Hg(2+) could alter some genes expression in the HPT axis which could be used as the potential biomarkers for evaluating the environmental Hg(2+)-induced stress in fish.

• MeSH
• beta podjednotka tyreotropinu genetika   MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• chlorid rtuťnatý toxicita   MeSH
• Cyprinidae genetika   metabolismus   MeSH
• hormon uvolňující kortikotropin genetika   MeSH
• larva účinky léků   genetika   metabolismus   MeSH
• LD50 MeSH
• regulace genové exprese účinky léků   MeSH
• symportéry genetika   MeSH
• thyreoglobulin genetika   MeSH
• thyroxin metabolismus   MeSH
• trijodthyronin metabolismus   MeSH
• tyreoidální hormony, receptory alfa genetika   MeSH
• tyreoidální hormony, receptory beta genetika   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Tributyltin (TBT) is a ubiquitous contaminant in aquatic environment, but the detailed mechanisms underlying the toxicity of TBT have not been fully understood. In this study, the effects of TBT on behavior, energy metabolism and endoplasmic reticulum (ER) stress were investigated by using Chinese rare minnow larvae. Fish larvae were exposed at sublethal concentrations of TBT (100, 400 and 800 ng/L) for 7 days. Compared with the control, energy metabolic parameters (RNA/DNA ratio, Na(+)-K(+)-ATPase) were significantly inhibited in fish exposed at highest concentration (800 ng/L), as well as abnormal behaviors observed. Moreover, we found that the PERK (PKR-like ER kinase)-eIF2α (eukaryotic translation initiation factor 2α) pathway, as the main branch was activated by TBT exposure in fish larvae. In short, TBT-induced physiological, biochemical and molecular responses in fish larvae were reflected in parameters measured in this study, which suggest that these biomarkers could be used as potential indicators for monitoring organotin compounds present in aquatic environment.

• MeSH
• chování zvířat účinky léků   MeSH
• Cyprinidae růst a vývoj   MeSH
• DNA vazebné proteiny genetika   metabolismus   MeSH
• endoribonukleasy genetika   metabolismus   MeSH
• energetický metabolismus účinky léků   MeSH
• kinasa eIF-2 genetika   metabolismus   MeSH
• larva účinky léků   metabolismus   MeSH
• stres endoplazmatického retikula účinky léků   MeSH
• transkripční faktor ATF4 genetika   metabolismus   MeSH
• transkripční faktory genetika   metabolismus   MeSH
• trialkylcínové sloučeniny toxicita   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Čína MeSH