Nuclear distribution of actin and myosin I depends on transcriptional activity of the cell
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As previous studies suggested, nuclear myosin I (NMI) and actin have important roles in DNA transcription. In this study, we characterized the dynamics of these two proteins during transcriptional activation in phytohemagglutinin (PHA) stimulated human lymphocytes. The stimulation led to strong up-regulation of NMI both on the mRNA and protein level, while actin was relatively stably expressed. The intranuclear distribution of actin and NMI was evaluated using immunogold labeling. In nucleoli of resting cells, actin was localized predominantly to fibrillar centers (FCs), while NMI was located mainly to the dense fibrillar component (DFC). Upon stimulation, FCs remained the main site of actin localization, however, an accumulation of both actin and NMI in the DFC and in the granular component was observed. In the nucleoplasm of resting lymphocytes, both actin and NMI were localized mostly in condensed chromatin. Following stimulation, the majority of both proteins shifted towards the decondensed chromatin. In transcriptionally active cells, both actin and NMI colocalized with nucleoplasmic transcription sites. These results demonstrate that actin and NMI are compartmentalized in the nuclei where they can dynamically translocate depending on transcriptional activity of the cells.
- MeSH
- aktiny metabolismus MeSH
- buněčné jadérko metabolismus účinky léků ultrastruktura MeSH
- buněčné jádro metabolismus účinky léků ultrastruktura MeSH
- financování organizované MeSH
- fytohemaglutininy farmakologie MeSH
- genetická transkripce imunologie MeSH
- lidé MeSH
- lymfocyty mikrobiologie účinky záření virologie MeSH
- messenger RNA metabolismus MeSH
- myosin typu I metabolismus MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- transmisní elektronová mikroskopie MeSH
- Check Tag
- lidé MeSH
... Control System Depends on Cyclically Activated Cyclin-Dependent Protein Kinases (Cdks) -- Cdk Activity ... ... Control Also Depends on Transcriptional Regulation The Cell-Cycle Control System Functions as a Network ... ... e 3i-Cdk and G-|/S-Cdk Activities E : : - s Cell Division: The DNA Damage Response I- 5 Have a Built-In ... ... CelF-Cell Adhesion Guides the :* Developing Tissues -- Transitions Depend on Control of -- I assedi Cadherins ... ... Actin: prraetjon With Cell-Cell Adhesion G’.e Eixthelia Mechanical Strength r : a Seal Between Cells ...
Sixth edition xxxiv, 1430 stran v různém stránkování : ilustrace (převážně barevné) ; 29 cm
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- molekulární biologie, molekulární medicína
- NLK Publikační typ
- učebnice vysokých škol
... 29 -- Multicellular Organization Depends on Cohesion Between Cells 29 -- Epithelial Sheets of Cells ... ... 554 -- Myosin is an Actin-activated ATPase 555 -- Myosin Heads Bind to Actin Filaments 555 -- Myosin ... ... Heads “Walk” Along Actin Filaments 556 -- Actin and Myosin Are Held in Position in the -- Myofibril ... ... Actin -- Polymerization and Thereby Affect Cell Behavior 572 -- The Polymerization of Actin and Tubulin ... ... Cells Is -- Ca2 +-dependent 587 -- Actin Filaments Are Often Anchored in Cell -- Membranes 588 -- Amoeboid ...
xxxix, 1146 s. : il., tab. ; 28 cm
- MeSH
- biologie buňky MeSH
- molekulární biologie MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- biologie
- cytologie, klinická cytologie
