To obtain enzymatic preparations with higher laccase activity levels from Funalia floccosa LPSC 232, available for use in several applications, co-cultures with six filamentous microfungi were tested. A laccase non-producing soil fungus, identified as Penicillium commune GHAIE86, showed an outstanding ability to increase laccase activity (3-fold as compared to that for monoculture) when inoculated in 6-day-old F. floccosa cultures. Maximum laccase production with the F. floccosa and P. commune co-culture reached 60 U/mL, or twice that induced by chemical treatments alone. Our study demonstrated that co-culture with soil fungi might be a promising method for improving laccase production in F. floccosa. Although the enhancement of laccase activity was a function of P. commune inoculation time, two laccase isoenzymes produced by F. floccosa remained unchanged when strains were co-cultured. These data are compatible with the potential of F. floccosa in agricultural applications in soil, whose enzyme machinery could be activated by soil fungi such as P. commune.

• MeSH
• časové faktory MeSH
• kokultivační techniky MeSH
• lakasa biosyntéza   chemie   metabolismus   MeSH
• mikrobiální interakce * MeSH
• Penicillium genetika   růst a vývoj   fyziologie   MeSH
• počet mikrobiálních kolonií MeSH
• Polyporaceae enzymologie   růst a vývoj   MeSH
• Polyporales MeSH
• půdní mikrobiologie MeSH
• Publikační typ
• časopisecké články MeSH