In the previous study, the artichoke leaf extract showed effective inhibition of AKR1B1, the first enzyme of polyol pathway, which reduces high level of glucose to osmotically active sorbitol, important for development of chronic diabetic complications. In the present study, the effect of artichoke leaf extract and of several participating phenols (caffeic acid, chlorogenic acid, quinic acid, and luteolin) was tested on sorbitol level in rat lenses exposed to high glucose ex vivo, on cytotoxicity as well as on oxidative stress in C2C12 muscle cell line induced by high glucose in vitro. The concentration of sorbitol was determined by enzymatic analysis, the cytotoxicity was provided by WST-1 test and intracellular content of reactive oxygen species was determined by fluorescence of 2'-7'-dichlorofluorescein probe. The extract and the compounds tested showed significant protection against toxic effects of high concentration of glucose in both models. On balance, the artichoke leaf extract thus represents a prospective preventive agent of development of chronic diabetic complications, probably due to phenols content, concerning preclinical and clinical studies.

• MeSH
• aldehydreduktasa antagonisté a inhibitory   MeSH
• Cynara scolymus chemie   MeSH
• glukosa farmakologie   MeSH
• inhibitory enzymů farmakologie   MeSH
• krysa rodu rattus MeSH
• kultivované buňky MeSH
• listy rostlin chemie   MeSH
• myši MeSH
• oční čočka účinky léků   metabolismus   MeSH
• orgánové kultury - kultivační techniky MeSH
• oxidační stres účinky léků   MeSH
• reaktivní formy kyslíku farmakologie   MeSH
• rostlinné extrakty farmakologie   MeSH
• sorbitol metabolismus   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH