Alternative matrix
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- MeSH
- dehydroepiandrosteron MeSH
- extracelulární matrix * fyziologie metabolismus MeSH
- fyziologie buňky MeSH
- hodnocení léčiv MeSH
- homeopatie * MeSH
- komplementární terapie MeSH
- léky na předpis MeSH
- lidé MeSH
- prolaktin MeSH
- receptory interleukinu-6 MeSH
- sorpční detoxikace MeSH
- xenobiotika MeSH
- Check Tag
- lidé MeSH
Práce je zaměřena na výrobu hypromelosových gelových matric lisováním granulátu předem připraveného termoplastickou granulací. Modelovým léčivem byl theofylin monohydrát. Jako tající pojivo se použil montanglykolový vosk v koncentraci 10 a 20 %. Z dosažených výsledků vyplývá, že proces termoplastické granulace zajišťuje vznik granulátu s vhodnou pórovitostí, tokovými vlastnosti a velikostí částic. V obou disolučních médiích (fosforečnanovém pufru pH 6,8 a umělé žaludeční šťávě pH 1,2) závisí uvolňování modelového léčiva na použitém viskozitním stupni hypromelosy (HPMC). Uvolňování theofylinu lze řídit také změnou vzájemného poměru HPMC K a montanglykolového vosku. Pokles vzájemného poměru se projeví zvýšením rychlosti liberace léčiva. Vzhledem k rozdílné rozpustnosti léčiva ve zvolených disolučních médiích se theofylin významně rychleji uvolňuje ve fosforečnanovém pufru pH 6,8 než v umělé žaludeční šťávě pH 1,2. Matrice stejného složení se připravily také přímým lisováním. Ze vzájemného srovnání disolučních profilů vzorků připravených různými výrobními postupy plyne, že použitá metoda přípravy nemá vliv na uvolňování léčiva z matrice.
The paper focuses on the formulation of HPMC K-matrix tablets by compression of granulates previously prepared by melt granulation. The model drug was theophylline monohydrate. Montanglycol wax was used as the solid lipid binder in a concentration of 10-20 %. With respect to the obtained results, thermoplastic granulation was found to ensure suitable porosity, flow, and particle size of the granulates. In both dissolution media (phosphate buffer pH 6.8 and artificial gastric juice pH 1.2), the release of the model drug is dependent on the HPMC viscosity grade used. The release rate can be modified by a change in the HPMC-to-montanglycol wax ratio. A decrease in this ratio increases the liberation of theophylline monohydrate. Due to different drug solubilities in the selected dissolution media, theophylline is released significantly faster in phosphate buffer pH 6.8 then in artificial gastric juice pH 1.2. The matrices of the same composition were prepared by direct compression; the comparison of dissolution profiles shows that the release of the active substance is not influenced by the employed method of manufacture.
- MeSH
- dehydroepiandrosteron * terapeutické užití MeSH
- extracelulární matrix * účinky léků MeSH
- farmakoterapie metody MeSH
- hodnocení léčiv MeSH
- hyaluronoglukosaminidasa * terapeutické užití MeSH
- interleukin-6 * terapeutické užití MeSH
- komplementární terapie MeSH
- lidé MeSH
- xenobiotika MeSH
- Check Tag
- lidé MeSH
RATIONALE: Distinguishing between individual bacterial strains below the species level is a challenge to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial profiling. We propose a quick method for improving strain differentiation of two Staphylococcus and one Bacillus species. METHODS: An alternative procedure to the extraction protocol recommended by Bruker Daltonics was developed. Ethanol-sterilized cells of six S. aureus and six S. haemolyticus strains were digested by trypsin using 2-min microwave irradiation and were then analyzed. Twenty-eight strains belonging to two ecotypes of B. subtilis were subjected to the same procedure to extend the scope of the method. RESULTS: S. aureus and S. haemolyticus strains, only partially distinguishable by the standard sample preparation procedure, were subjected to microwave-assisted tryptic digestion. The repeatability of the procedure was checked in three experiments accomplished at weekly intervals. Clear distinction of the strains was achieved by cluster analysis. The differentiation of B. subtilis ecotypes was also improved significantly by the digestion method. The discriminatory power of the novel method was supported by an increase in the number of strain-specific peaks, as compared to the standard method. CONCLUSIONS: The method modulates the discriminatory power of MALDI-TOF MS profiling. The differentiation of a set of S. aureus, S. haemolyticus and B. subtilis strains was improved significantly after microwave-accelerated tryptic digestion of the cellular material.
- MeSH
- Bacillus chemie klasifikace MeSH
- mikrovlny MeSH
- molekulární typizace metody MeSH
- shluková analýza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- Staphylococcus chemie klasifikace MeSH
- trypsin MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Úvod: Obnovení stability poraněného pánevního kruhu představuje základní předpoklad k zajištění dobrého funkčního výsledku. Existuje množství stabilizačních technik včetně množství technik perkutánních a miniinvazivních. Cílem kazuistiky bylo představit alternativní možnost miniinvazivní fixace předního pánevního segmentu použitím spondylochirugického instrumentária ©MATRIX Spine Systém. Materiál a metoda: Kazuistika popisující postup miniinvazivní stabilizace předního pánevního segmentu supraacetábulárně zavednými polyaxiálními šrouby s pevnou, ale tvarovatelnou spojovací tyčí u obézního pacienta, po embolizaci a. iliaca interna s mohutným hematomem v oblasti podbříšku. Výsledky: Nekomplikovaný průběh hojení bez nutnosti extrakce kovu. Závěr: Miniinvazivní stabilizace předního pánevního segmentu pomocí spondylochirugického instrumentária ©MATRIX Spine System rozšiřuje repertoár možností fixace při poranění pánevního kruhu.
Introduction: Restoring stability of the injured pelvic ring is a precondition for ensuring a good functional outcome. There are a number of stabilizing techniques including some percutaneous and minimally invasive techniques. The aim of this case-report was to present an alternative option of minimally invasive fixation of the anterior segment of the pelvis using ©MATRIX Spine System for spondylosurgical stabilization. Materials and Methods: This case report describes an alternative procedure of minimally invasive stabilization of the anterior pelvic segment with polyaxial screws fixed in the supra-acetabular region with a firm but fle- xible connecting rod in an obese patient with polytrauma, after embolization of the internal illiac artery with a massive hematoma in the lower abdomen. Results: Uncomplicated healing process. After 12 months thepatient is without difficulties and there is no hardware-related irritation. He does not request hardware removal. Conclusion: Minimally invasive pelvic stabilization of the anterior segment using the ©MATRIX Spine System expands the repertoire of fixation options for pelvic ring injuries.
- Klíčová slova
- MATRIX Spine System, symfyzeolýza, zlomenina pánve,
- MeSH
- dopravní nehody MeSH
- lidé MeSH
- miniinvazivní chirurgické výkony MeSH
- pánev * chirurgie zranění MeSH
- polytrauma etiologie chirurgie MeSH
- senioři MeSH
- vnitřní fixace fraktury * MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- kazuistiky MeSH
The bacteriophage K1/420 is a member of genus Kayvirus that was extensively studied as an alternative treatment to combat bacterial infections caused by antibiotic-resistant Staphylococcus aureus strains. Despite the promise of phage therapy, the development of clinical applications of phages is facing regulatory and technical hurdles before it can receive acceptance in the Western World. Suitable simple and accurate diagnostic techniques to control the quality of the phage, which would satisfy the requirements of regulatory authorities are still being discussed. Here, we present the conditions for the simultaneous separation and detection of phage K1/420 and S. aureus by CZE and by CIEF were found, and the phage isoelectric point was determined to be 3.6. After removing the cell debris, the phage was successfully purified from the crude phage lysate and pre-concentrated by preparative isoelectric focusing. Its zone was localized by the positions of colored pI markers in the cellulose bed. The phage from the harvested zone had a decreased ability to infect its host. However, it was suitable for its separation, detection and identification by capillary electrophoretic methods, MALDI-TOF MS and electron microscopy.
Heart failure (HF) is life-threatening disease due to electro-mechanical dysfunction associated with hemodynamic overload, while alterations of extracellular matrix (ECM) along with perturbed connexin-43 (Cx43) might be key factors involved. We aimed to explore a dual impact of pressure, and volume overload due to aorto-caval fistula (ACF) on Cx43 and ECM as well as effect of renin-angiotensin blockade. Hypertensive Ren-2 transgenic rats (TGR) and normotensive Hannover Sprague-Dawley rats (HSD) that underwent ACF were treated for 15-weeks with trandolapril or losartan. Blood serum and heart tissue samples of the right (RV) and left ventricles (LV) were used for analyses. ACF-HF increased RV, LV and lung mass in HSD and to lesser extent in TGR, while treatment attenuated it and normalized serum ANP, BNP-45 and TBARS. Cx43 protein and its ser368 variant along with PKCε were lower in TGR vs HSD and suppressed in both rat strains due to ACF but prevented more by trandolapril. Pro-hypertrophic PKCδ, collagen I and hydroxyproline were elevated in TGR and increased due to ACF in both rat strains. While SMAD2/3 and MMP2 levels were lower in TGR vs HSD and reduced due to ACF in both strains. Findings point out the strain-related differences in response to volume overload. Disorders of Cx43 and ECM signalling may contribute not only to HF but also to the formation of arrhythmogenic substrate. There is benefit of treatment with trandolapril and losartan indicating their pleiotropic anti-arrhythmic potential. It may provide novel input to therapy.
- MeSH
- extracelulární matrix MeSH
- hypertenze * MeSH
- konexin 43 genetika MeSH
- krevní tlak MeSH
- krysa rodu rattus MeSH
- losartan farmakologie MeSH
- píštěle * MeSH
- potkani Sprague-Dawley MeSH
- potkani transgenní MeSH
- renin MeSH
- srdeční selhání * MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Staphylococcus aureus is a major causative agent of infections associated with hospital environments, where antibiotic-resistant strains have emerged as a significant threat. Phage therapy could offer a safe and effective alternative to antibiotics. Phage preparations should comply with quality and safety requirements; therefore, it is important to develop efficient production control technologies. This study was conducted to develop and evaluate a rapid and reliable method for identifying staphylococcal bacteriophages, based on detecting their specific proteins using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling that is among the suggested methods for meeting the regulations of pharmaceutical authorities. Five different phage purification techniques were tested in combination with two MALDI-TOF MS matrices. Phages, either purified by CsCl density gradient centrifugation or as resuspended phage pellets, yielded mass spectra with the highest information value if ferulic acid was used as the MALDI matrix. Phage tail and capsid proteins yielded the strongest signals whereas the culture conditions had no effect on mass spectral quality. Thirty-seven phages from Myoviridae, Siphoviridae or Podoviridae families were analysed, including 23 siphophages belonging to the International Typing Set for human strains of S. aureus, as well as phages in preparations produced by Microgen, Bohemia Pharmaceuticals and MB Pharma. The data obtained demonstrate that MALDI-TOF MS can be used to effectively distinguish between Staphylococcus-specific bacteriophages.
- MeSH
- biologické přípravky izolace a purifikace MeSH
- chemická frakcionace metody MeSH
- lidé MeSH
- replikace viru MeSH
- shluková analýza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice * metody MeSH
- stafylokokové bakteriofágy klasifikace metabolismus MeSH
- Staphylococcus aureus virologie MeSH
- virové proteiny analýza chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
UNLABELLED: INTRODUCTION Alternative fusion expanders are in clinical use for instrumented posterolateral fusion (PLF) to avoid donor site morbidity in autologous bone graft (ABG) harvesting. Purpose of this study was to evaluate demineralized bone matrix (DBM) in PLF as alternative to the gold standard of ABG in acute traumatic vertebral body fractures of the thoracolumbar spine. MATERIAL AND METHODS We retrospectively identified 101 patients with acute traumatic vertebral body fractures of the thoracic and lumbar spine who were treated with instrumented PLF in our level one trauma center between 2005 and 2011. Patients with a primary paraplegia, osteodepriving disease or loss to follow-up had been excluded. Until August 2008, autologous bone graft harvested from the posterior iliac crest was used in PLF (control group n = 46). Starting September 2008, DBM was used as fusion expander in PLF (study group n = 16). Clinical and radiological evaluation was performed with a minimum followup of 18 months to assess the clinical and radiological outcome. RESULTS We found a fusion rate of 94% in patients undergoing PLF with the use of DBM and 100% with the use of ABG. There was one major complication of deep infection in the DBM group and two cases of superficial wound infection in the ABG group. We discovered a trend of reduced operating time with the use of DBM. CONCLUSIONS DBM leads to a similar fusion rate as the use of ABG in patients undergoing PLF for acute traumatic vertebral body fractures of the thoracic and lumbar spine. DBM is associated with reduced operating time. LEVEL OF EVIDENCE III: case-control study Key words: demineralized bone matrix instrumented posterolateral fusion, acute traumatic vertebral body fracture, thoracolumbar spine, autologous bone graft.
- MeSH
- autologní transplantace MeSH
- bederní obratle zranění chirurgie MeSH
- biokompatibilní materiály terapeutické užití MeSH
- dospělí MeSH
- fraktury páteře chirurgie MeSH
- fúze páteře metody MeSH
- hojení fraktur MeSH
- hrudní obratle zranění chirurgie MeSH
- kostní matrix transplantace MeSH
- lidé středního věku MeSH
- lidé MeSH
- os ilium transplantace MeSH
- osteointegrace MeSH
- retrospektivní studie MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Diagnostic methods used in the contemporary medical practice consist of a combination of distressing invasive (bronchial biopsy, bronchoalveolar lavage) and semiinvasive (induced sputum technique) methods. Monitoring of specific molecules produced during pathological processes in biological matrices is a relatively new technique which represents an alternative, entirely non-invasive and comfortable method. The principle is based on the quantification of specific substances – "biomarkers", which are considered to be objectively measurable indicators of a physiological/pathological condition of the organism. In contrast with non-specific matrices such as blood plasma (reflecting the state of the whole organism), the exhaled breath condensate is a specific matrix. Concentration levels of biomarkers in the latter matrix point at pathological processes only in the airways and lungs. Typical molecules to be monitored include hydrogen peroxide, leukotrienes, prostaglandins, lipoxins and prostanoids.
- Klíčová slova
- kondenzát vydechovaného vzduchu,
- MeSH
- biologické markery analýza MeSH
- chronická obstrukční plicní nemoc diagnóza MeSH
- diagnostické techniky dýchacího ústrojí MeSH
- ELISA MeSH
- hmotnostní spektrometrie MeSH
- kyseliny prostanové analýza MeSH
- leukotrieny analýza MeSH
- lidé MeSH
- lipoxiny analýza MeSH
- metody pro podporu rozhodování MeSH
- prognóza MeSH
- prostaglandiny analýza MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
