- MeSH
- Kidney Failure, Chronic etiology surgery complications MeSH
- Cisplatin administration & dosage pharmacology toxicity MeSH
- Animal Experimentation statistics & numerical data MeSH
- Financing, Organized MeSH
- Gentamicins administration & dosage pharmacology toxicity MeSH
- Hypertension diagnosis etiology MeSH
- Cardiovascular Diseases etiology complications MeSH
- Comorbidity MeSH
- Blood Pressure radiation effects MeSH
- Rats MeSH
- Aristolochic Acids administration & dosage pharmacology toxicity MeSH
- Nephrectomy methods statistics & numerical data MeSH
- Risk Factors MeSH
- Heart Rate drug effects MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Comparative Study MeSH
The growth in the experimental research of facilities to support extracorporeal circulation requires the further development of models of acute heart failure that can be well controlled and reproduced. Two types of acute heart failure were examined in domestic pigs (Sus scrofa domestica): a hypoxic model (n=5) with continuous perfusion of the left coronary artery by hypoxic deoxygenated blood and ischemic model (n=9) with proximal closure of the left coronary artery and controlled hypoperfusion behind the closure. The aim was a severe, stable heart pump failure defined by hemodynamic parameters changes: a) decrease in cardiac output by at least 50 %; b) decrease in mixed venous blood saturation to under 60 %; c) left ventricular ejection fraction below 25 %; and d) decrease in flow via the carotid arteries at least 50 %. Acute heart failure developed in the first group in one animal with no acute mortality and in the second group in 8 animals with no acute mortality. In the case of ischemic model the cardiac output fell from 6.70+/-0.89 l/min to 2.89+/-0.75 l/min. The saturation of the mixed venous blood decreased from 83+/-2 % to 58+/-8 %. The left ventricular ejection fraction decreased from 50+/-8 % to 19+/-2 %. The flow via the carotid arteries decreased from 337+/-78 ml/min to 136+/-59 ml/min (P
- MeSH
- Acute Disease MeSH
- Hemodynamics physiology MeSH
- Disease Models, Animal * MeSH
- Swine MeSH
- Heart Failure mortality physiopathology MeSH
- Severity of Illness Index * MeSH
- Sus scrofa MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
The rat model of myocardial infarction is characterized by progressive cardiac hypertrophy and failure. Rats with infarcts greater than 30% of the left ventricle exhibited early and moderate stages of heart failure 4 and 8 weeks after the occlusion of the left coronary artery, respectively. As heart failure is usually associated with remodeling of the extracellular matrix, a histological and biochemical study of cardiac collagenous proteins was carried out using failing hearts. Total collagen content in the right ventricle increased at 2, 4, and 8 weeks following occlusion of the left coronary artery whereas such a change in viable left ventricle was seen after 4 and 8 weeks. Total cardiac hydroxyproline concentration was increased in both right and left ventricular samples from the infarcted animals when compared to those of control; this increase was due to elevation of pepsin-insoluble collagen fraction. The myocardial noncollagenous/collagenous protein ratio was decreased in experimental right and left ventricular samples when compared to control samples. These findings suggest that an increase in cross-linking of cardiac collagen as well as disparate synthesis of collagenous and noncollagenous proteins occurs in this model of congestive heart failure.
Cílem naší studie bylo vytvořit snadno reprodukovatelný model akutního selhání jater (ASJ) u miniprasete, který umožní testovat eliminační podpůrné systémy. Cílem tohoto sdělení je popsat chirurgickou techniku s anesteziologickým postupem a doložit výsledky experimentu na skupině laboratorních zvířat. Metoda: Model ASJ jsme provedli chirurgickou devaskularizací jater: ligatura a. hepatica propria a v. portae s vytvořením portokavální anastomózy end-to-side. Zvířata byla během experimentu analgosedovaná a napojená na umělou plieni ventilaci. Monitorovali jsme biochemické ukazatele selhání jater, ICP a parametry hemodynamiky. Za počátek jaterního selhání jsme považovali nástup hypoglykemie pod hodnoty 3,5 mmol/1 a vzestup intrakraniálního tlaku (ICP). Materiál: Do experimentu jsme zahrnuli 20 miniprasat hmotnosti 25-30 kg rozdělených do 3 skupin: I - 10 zvířat do skupiny s ASJ, II - 5 zvířat s vyvolaným ASJ, u kterých jsme kromě standardní monitorace měrili ICP a III. 5 zvířat bez ASJ - kontrolní skupina. Výsledky: ProkázaU jsme signifikantní rozdíl v hladině AST (33,44 ± 39,96 vs. 1,56 ± 0,50 mmol/1), laktátu (2,97 ± 1,16 vs. 1,18 ± 0,61 mmol/1), a amoniaku (264,3 ± 93,05 vs. 42,5 ± 12,98 ^imol/1) u skupiny s ASJ a skupiny kontrolní (p < 0,01) 6 hodin po operaci a signifikantní změny v hodnotě glykemie a intrakraniálního tlaku (ICP) 4 hodiny po operaci. Ke zvýšení tepové frekvence, krevního tlaku, poklesu středního arteriálního tlaku (MAP) a poklesu indexu systémové cévní rezistence (SVRI) u skupiny s ASJ došlo po 6 hodinách po operaci proti hodnotám vstupním, s významnými rozdíly mezi skupinami ASJ a kontrolm' až ve 12. hodině po výkonu (SVRI: 953 ASJ vs. 1658 kontrola, p _ 0,05, MAP: 58,1 ASJ vs. 76 kontrola, p _ 0,05). Nebyly zaznamenány statisticky významné rozdíly v srdečním indexu mezi skupinou ASJ a skupinou kontrolní. Zvířata s ASJ přežívala v průměru 13 hodin po operaci.
Aim; The aim of this study was to create an easily reproducible model of the acute hepatic failure (ASJ) in a mmipig, which may allow to test supporting eliminating systems. The aim of this report was to describe the surgical technique with the anaesthesiological procedure and to demonstrate the experimental results on a group of the laboratory animals. Methodology: The ASJ was mocked using surgical devascularization of the liver: a ligature of the a. hepatica propria and v. portae, creating a portocaval anastomosis end-to-side. The animals were analgosedated during the experiment and were on a ventilatory support. Biochemical indicators of the hepatic failure, the ICP and the haemodynamics parameters were monitored. Hypoglycaemia with levels below 3.5 mmol/1 and any increase of the intracranial pressure (ICP) were considered the onset of the hepatic failure. Material: 20 minipigs weighing 25-30 kg and divided in 3 groups, were included in the experiment: I - 10 animals were assigned to the acute hepatic failure (ASJ) group, II - 5 animals with the mock acute hepatic failure (ASJ), whose ICP was monitored in conjunction with the standard monitoring and III. 5 animals without the acute hepatic failure (ASJ) - a control group.Results: We proved significant differences in the AST levels (33.44 ± 39.96 vs. 1.56 ± 0.50 mmol/1), the lactate levels (2.97 ± 1.16 vs. 1.18 ± 0.61 mmol/1), and the ammonium levels (264.3 ± 93.05 vs. 42.5 ± 12.98 Hmol/1) in the acute hepatic failure (ASJ) group compared with the control group (p < 0.01) 6 hours after the surgery and ign piificant changes in the glycaemia levels and the intracranial pressure (ICP) measurements 4 hours after the surgical procedure. Increase in the pulse frequency, the blood pressure, decrease of the mean arterial pressure (MAP) and decrease in the systemic venous resistence index (SVRI) in the acute hepatic failure (ASJ) group was recorded 6 hours after the procedure compared with the initial findings, with significant differences between the acute hepatic failure (ASJ groups and the control group as late as during the 12th hour following the procedure (SVRI: 953 ASJ vs. 1658 control, p _ 0.05, MAP: 58.1 ASJ vs. 76 control, p _ 0.05). No statistically significant differences in the heart index between the acute hepatic failure (ASJ) group and the control group were recorded. he animals with the acute hepatic failure (ASJ) survived 13 hours following the surgical procedure, on average.
... Contents -- Acknowledgments 5 -- Abbreviations 7 -- Abstract 9 -- 1 Introduction 11 -- 1.1 Heart failure ... ... failure models 17 -- 1.3 ECLS 18 -- 1.3.1 Definition of ECLS 18 -- 1.3.2 History of ECLS 19 -- 1.3.3 ... ... of chronic heart failure 41 -- 4.2 Animal model of acute HF induced by regional coronary hypoxemia 44 ... ... -- 4.3 Animal model of acute HF induced by global coronary hypoxemia 45 -- 4.4 Animal model of right-sided ... ... of acute HF model induced by global coronary hypoxemia 56 -- 5.4 Characteristics of right-sided HF model ...
Acta Universitatis Carolinae. Medica. Monographia, ISSN 0567-8250 161
92 stran : ilustrace, tabulky ; 23 cm
- MeSH
- Adaptation, Physiological MeSH
- Hemodynamics MeSH
- Cardiac Pacing, Artificial methods MeSH
- Extracorporeal Membrane Oxygenation methods MeSH
- Disease Models, Animal MeSH
- Heart Ventricles MeSH
- Heart Failure surgery MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- experimentální medicína
- kardiologie
- kardiochirurgie
- NML Publication type
- studie
