The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogs--idebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ1 were found to be more efficient in the scavenging activity (IC50: 0.052 and 0.075 microM, respectively) than CoQ3 (IC50: 45.8 microM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation.

• MeSH
• antioxidancia farmakologie   MeSH
• benzochinony farmakologie   MeSH
• ferrikyanidy farmakologie   MeSH
• financování organizované MeSH
• glycerolfosfáty metabolismus   MeSH
• hnědá tuková tkáň metabolismus   ultrastruktura   MeSH
• křečci praví MeSH
• křeček rodu Mesocricetus MeSH
• mitochondrie metabolismus   účinky léků   MeSH
• peroxid vodíku metabolismus   MeSH
• reaktivní formy kyslíku MeSH
• scavengery volných radikálů MeSH
• spotřeba kyslíku účinky léků   MeSH
• sukcináty metabolismus   MeSH
• ubichinon metabolismus   MeSH
• zvířata MeSH
• Check Tag
• křečci praví MeSH
• mužské pohlaví MeSH
• zvířata MeSH