Lin, Chorng-Horng*
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Paclobutrazol, (2RS, 3RS)-1-(4-chlorophenyl)-4, 4-dimethyl-2-(1H-1,2,4-triazol-1-yl) pentan-3-ol, is a plant growth retardant that mainly inhibits gibberellins (GAs) biosynthesis. In agricultural practice, paclobutrazol is applied to arrest vegetative growth so as to increase the reproductive growth of many orchard fruit, as well as grain crops. However, due to its over-application and chemical stability, paclobutrazol accumulates in soil and inhibits the growth of subsequent crops, especially those grown for vegetative purposes. The present study focused mainly on the changes in the soil bacterial community following application of paclobutrazol. Mung bean (Vigna radiata) plants were treated with paclobutrazol and cultivated for three consecutive seasons. Soil samples were collected and analyzed by denaturing gradient gel electrophoresis (DGGE) using 16S rDNA gene fragments and clone library analyses. The results obtained through clustering and clonal sequencing analysis showed that the bacterial community was affected by paclobutrazol, and in addition, was more diverse in the third stage of mung bean plant cultivation. The results of the study showed that paclobutrazol affected bacterial composition, and the population of bacteria varied greatly across time.
- MeSH
- Bacteria klasifikace účinky léků genetika izolace a purifikace MeSH
- biodiverzita MeSH
- časové faktory MeSH
- květy účinky léků růst a vývoj MeSH
- mikrobiota účinky léků MeSH
- půda chemie MeSH
- půdní mikrobiologie * MeSH
- RNA ribozomální 16S genetika MeSH
- shluková analýza MeSH
- triazoly farmakologie MeSH
- vigna účinky léků růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH