• Klíčová slova
• hibernace přirozená - savci - sborníky prací, fyziologové - konference (Fallen Leaf Lake,1985) - sborníky prací, chlad - pobyt v - adaptace biochemická, fyziologická - sborníky prací,

• Konspekt
• Veřejné zdraví a hygiena
• NLK Obory
• environmentální vědy
• patologie
• biochemie
• fyziologie

• Konspekt
• Lékařské vědy. Lékařství
• NLK Obory
• patologie
• vnitřní lékařství
• fyziologie

BACKGROUND: The phlebotomine sand fly Phlebotomus perniciosus (Diptera: Psychodidae, Phlebotominae) is a major Old World vector of the protozoan Leishmania infantum, the etiological agent of visceral and cutaneous leishmaniases in humans and dogs, a worldwide re-emerging diseases of great public health concern, affecting 101 countries. Despite the growing interest in the study of this sand fly species in the last years, the development of genomic resources has been limited so far. To increase the available sequence data for P. perniciosus and to start studying the molecular basis of the sexual differentiation in sand flies, we performed whole transcriptome Illumina RNA sequencing (RNA-seq) of adult males and females and de novo transcriptome assembly. RESULTS: We assembled 55,393 high quality transcripts, of which 29,292 were unique, starting from adult whole body male and female pools. 11,736 transcripts had at least one functional annotation, including full-length low abundance salivary transcripts, 981 transcripts were classified as putative long non-coding RNAs and 244 transcripts encoded for putative novel proteins specific of the Phlebotominae sub-family. Differential expression analysis identified 8590 transcripts significantly biased between sexes. Among them, some show relaxation of selective constraints when compared to their orthologs of the New World sand fly species Lutzomyia longipalpis. CONCLUSIONS: In this paper, we present a comprehensive transcriptome resource for the sand fly species P. perniciosus built from short-read RNA-seq and we provide insights into sex-specific gene expression at adult stage. Our analysis represents a first step towards the identification of sex-specific genes and pathways and a foundation for forthcoming investigations into this important vector species, including the study of the evolution of sex-biased genes and of the sexual differentiation in phlebotomine sand flies.

• MeSH
• hmyz - vektory genetika   MeSH
• Leishmania infantum genetika   patogenita   MeSH
• leishmanióza viscerální genetika   parazitologie   MeSH
• lidé MeSH
• Phlebotomus genetika   parazitologie   MeSH
• pohlavní dimorfismus MeSH
• psi MeSH
• sekvence aminokyselin MeSH
• transkriptom genetika   MeSH
• vysoce účinné nukleotidové sekvenování MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• psi MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH