Na+/K+ ATPase
Dotaz
Zobrazit nápovědu
- MeSH
- kardiomyocyty enzymologie fyziologie MeSH
- protein - isoformy klasifikace MeSH
- sodíko-draslíková ATPasa fyziologie MeSH
- Publikační typ
- přehledy MeSH
- MeSH
- Ca(2+)-Mg(2+)-ATPasa metabolismus MeSH
- hypothalamus metabolismus MeSH
- krysa rodu rattus MeSH
- lithium aplikace a dávkování metabolismus terapeutické užití MeSH
- modely nemocí na zvířatech MeSH
- mozková kůra metabolismus MeSH
- sodíko-draslíková ATPasa metabolismus MeSH
- thalamus metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
- srovnávací studie MeSH
Na(+)/K(+)-ATPase (NKA) is an essential cation pump protein responsible for the maintenance of the sodium and potassium gradients across the plasma membrane. Recently published high-resolution structures revealed amino acids forming the cation binding sites (CBS) in the transmembrane domain and variable position of the domains in the cytoplasmic headpiece. Here we report molecular dynamic simulations of the human NKA α1β1 isoform embedded into DOPC bilayer. We have analyzed the NKA conformational changes in the presence of Na(+)- or K(+)-cations in the CBS, for various combinations of the cytoplasmic ligands, and the two major enzyme conformations in the 100 ns runs (more than 2.5 μs of simulations in total). We identified two novel cytoplasmic pathways along the pairs of transmembrane helices TM3/TM7 or TM6/TM9 that allow hydration of the CBS or transport of cations from/to the bulk. These findings can provide a structural explanation for previous mutagenesis studies, where mutation of residues that are distal from the CBS resulted in the alteration of the enzyme affinity to the transported cations or change in the enzyme activity.
- MeSH
- draslík metabolismus MeSH
- fosfatidylcholiny metabolismus MeSH
- kationty metabolismus MeSH
- konformace proteinů MeSH
- lidé MeSH
- lipidové dvojvrstvy metabolismus MeSH
- simulace molekulární dynamiky MeSH
- sodík metabolismus MeSH
- sodíko-draslíková ATPasa chemie metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Acta pharmacologica et toxicologica, ISSN 0065-1508 vol. 52, suppl. I, 1983
19 s. : grafy ; 24 cm
