• Conspectus
• Ortopedie. Chirurgie. Oftalmologie
• NML Fields
• chirurgie

• MeSH
• Sentinel Lymph Node Biopsy MeSH
• Molecular Diagnostic Techniques methods   MeSH
• Keratin-19 MeSH
• Cohort Studies MeSH
• Humans MeSH
• Lymph Node Excision MeSH
• Lymphatic Metastasis diagnosis   MeSH
• Mastectomy MeSH
• Breast Neoplasms * surgery   diagnosis   MeSH
• Neoplasm Grading MeSH
• Nucleic Acid Amplification Techniques methods   MeSH
• Check Tag
• Humans MeSH
• Female MeSH

The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical relevance of the results was evaluated based on relation to prognosis; the disease-free interval (DFI) and overall survival (OS) were analyzed. During radical surgical treatment, a total of 1426 lymph nodes were obtained from 100 patients, creating 472 groups of nodes (4-5 groups per patient) and examined by H&E, IHC CK19 and OSNA. The median follow-up was 44 months. Concordant results on the lymph node status of the H&E, IHC CK19 and OSNA examinations were reported in 78% of patients. We recorded shorter OS in patients with positive results provided by both OSNA and H&E. The study demonstrated a higher percentage of detected micrometastases in lymph nodes by the OSNA method. However, the higher sensitivity of the OSNA, with the cut-off value 250 copies of mRNA of CK19/μL, resulted in a lower association of OSNA positivity with progress of the disease compared to H&E. Increasing the cut-off to 615 copies resulted in an increase in concordance between the OSNA and H&E, which means that the higher cut-off is more relevant in the case of lung tumors.

• MeSH
• Immunohistochemistry methods   MeSH
• Keratin-19 genetics   MeSH
• Humans MeSH
• Lymphatic Metastasis * MeSH
• Lymph Nodes pathology   MeSH
• Neoplasm Metastasis MeSH
• Biomarkers, Tumor genetics   MeSH
• Lung Neoplasms diagnosis   pathology   surgery   MeSH
• Follow-Up Studies MeSH
• Disease-Free Survival MeSH
• Prognosis MeSH
• Prospective Studies MeSH
• Neoplasm Staging MeSH
• Nucleic Acid Amplification Techniques methods   MeSH
• Treatment Outcome MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Comparative Study MeSH

BACKGROUND: Detection of tumor cells in lymph nodes (LNs) removed during the treatment of pulmonary tumor by radical surgery is limited by the possibilities of standard histopathological methods. The goal of this study was to obtain more accurate pTNM status by a more sensitive detection of micrometastases in LNs. METHODS: A total of 885 LNs, an average of 13.8 LNs per patient, were removed during 64 surgeries. LNs from the same zone were pooled together as a group, five groups of LNs were examined in each patient. A total of 320 groups of LNs were examined. One-step nucleic acid amplification (OSNA) method was compared to standard histopathological examination with haematoxylin-eosin (H&E) staining and CK19 immunohistochemistry, specifically by an ultimate analysis of all intraoperatively removed LNs. RESULTS: Identical results for H&E and OSNA examinations were recorded in 286 groups of LNs (89.4%). In total, positive examinations were recorded in 27 groups of LNs (8.4%) using the OSNA method, which were H&E negative. In seven groups of LNs (2.2%), the H&E examination was positive, while OSNA method produced negative results. CONCLUSIONS: The OSNA examination led to a higher pTNM stage classification in 14 (21.9%) patients. The clinical significance remains the subject of follow-up research.

• MeSH
• Adenocarcinoma genetics   secondary   surgery   MeSH
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Lymph Nodes metabolism   pathology   surgery   MeSH
• RNA, Messenger MeSH
• Neoplasm Micrometastasis MeSH
• Biomarkers, Tumor genetics   MeSH
• Lung Neoplasms genetics   pathology   surgery   MeSH
• Follow-Up Studies MeSH
• Carcinoma, Non-Small-Cell Lung genetics   secondary   surgery   MeSH
• Prognosis MeSH
• Prospective Studies MeSH
• Aged MeSH
• Carcinoma, Squamous Cell genetics   secondary   surgery   MeSH
• Nucleic Acid Amplification Techniques methods   MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Aged MeSH
• Female MeSH
• Publication type
• Journal Article MeSH

Cíl studie: Článek popisuje současné možnosti využití molekulárně biologické metody One-Step Nucleic Acid Amplification (OSNA) v peroperačním zpracování sentinelových lymfatických uzlin v onkologické operativě. Principem vyšetření je stanovení cytokeratinu 19 jako markeru metastatického postižení ve vzorku lymfatické tkáně. Typ studie: Přehledový článek. Název a sídlo pracoviště: Gynekologicko-porodnická klinika, Fakultní nemocnice Plzeň a Lékařská fakulta UK v Plzni; Ústav biologie, Lékařská fakulta UK v Plzni; Oddělení imunochemické diagnostiky, Fakultní nemocnice Plzeň a Lékařská fakulta UK v Plzni; Šiklův ústav patologie, Fakultní nemocnice Plzeň a Lékařská fakulta UK v Plzni. Metodika: Literární rešerše literatury dostupné na databázi PubMed a publikované do dubna roku 2017. Jako klíčových slov bylo použito oficiálně využívané zkratky OSNA i celého názvu metody One-Step Nucleic Acid Amplification. Závěr: Využití metody OSNA s detekcí cytokeratinu 19 v sentinelových uzlinách jako markeru metastatického postižení představuje v současné době akceptovanou formu peroperačního zpracování sentinelových uzlin u karcinomu prsu a kolorektálního karcinomu. Dosud publikovaná data naznačují možnost úspěšného využití tohoto způsobu vyšetření sentinelových uzlin i u ostatních zhoubných novotvarů s lymfogenním šířením. Jedná se například o karcinomy štítné žlázy, žaludku, plic, dělohy, hlavy a krku. K prosazení metody OSNA i u těchto malignit je však zapotřebí většího množství publikovaných dat.

Objective: The article summarizes current possibilities of usage of the One-Step Nucleic Acid Amplification method (OSNA) in the perioperative management of sentinel lymph nodes in oncologic surgery. The principle of this method is the detection of cytokeratin 19 (CK19) in the lymphatic tissue as a marker of the metastatic spread. Design: Review article. Settings: Department of Obstetrics and Gynaecology, University Hospital Pilsen, Faculty of Medicine in Pilsen, Charles University, Prague; Department of Biology, Faculty of Medicine in Pilsen, Charles University, Prague; Department of Immunochemistry, University Hospital Pilsen, Faculty of Medicine in Pilsen, Charles University, Prague; Sikl´s Department of Pathology, University Hospital Pilsen, Faculty of Medicine in Pilsen, Charles University, Prague. Methods: The review of the literature published until the end of April 2017 available on the PubMed database was performed. The official abbreviation OSNA and the full name of the method One-Step Nucleic Acid Amplification was used for search in this database. Conclusion: The usage of the OSNA method with the detection of CK 19 in the sentinel lymph nodes as a marker of metastatic spread to the lymphatic tissue currently represents an acceptable form of perioperative sentinel lymph node management in patients with breast and colorectal cancer. Until now published data are pointing towards possible successful application of this method in sentinel lymph node management in patients with some other malignancies, such as thyroid carcinoma, gastric cancer, uterus cancer and head and neck cancer. More data is needed to establish this method also in those neoplasms.

• Keywords
• OSNA, CK19,
• MeSH
• Molecular Diagnostic Techniques * methods   MeSH
• Humans MeSH
• Neoplasms * diagnosis   MeSH
• Intraoperative Period MeSH
• Sentinel Lymph Node MeSH
• Check Tag
• Humans MeSH
• Publication type
• Review MeSH

• Publication type
• Meeting Abstract MeSH

• MeSH
• Cataract Extraction history   MeSH
• Cataract history   MeSH
• Humans MeSH
• Check Tag
• Humans MeSH

• MeSH
• Outpatient Clinics, Hospital MeSH
• Physicians MeSH
• Medicine in Literature MeSH
• Aerospace Medicine MeSH
• Publication type
• Fictional Work MeSH

• Conspectus
• Německá próza, německy psaná
• NML Fields
• humanitní vědy a umění
• lékařství

BACKGROUND AND OBJECTIVES: Utilisation of the one-step nucleic acid amplification (OSNA) molecular biology method for the detection of the metastatic involvement of sentinel lymph nodes (SLNs) in endometrial cancer (EC) patients. A comparison with histopathological ultrastaging and a description of the clinical consequences. METHODS: Surgically treated EC patients underwent detection of SLNs. Nodes greater than 5 mm were cut into sections 2-mm thick parallel to the short axis of the node. Odd sections were examined according to the OSNA method, while even ones according to an appropriate ultrastaging protocol. Nodes less than or equal to 5 mm were cut into halves along the longitudinal axis with one half examined according to the OSNA method and the other half by ultrastaging. RESULTS: Fifty-eight patients were included and 135 SLNs were acquired. Both ultrastaging and OSNA agreed on 116 results. According to the OSNA method, 20.69% more patients were classified into International Federation of Gynecology and Obstetrics (FIGO) stage III. When comparing the results of the OSNA method to the conclusions of ultrastaging as a reference method, sensitivity of 90.9%, specificity of 85.5% and concordance of 85.9% were attained. CONCLUSIONS: The results of the OSNA method showed a higher frequency of detection of micrometastases and included 20.69% more patients into FIGO stage III.

• MeSH
• Adenocarcinoma, Clear Cell genetics   secondary   surgery   MeSH
• Sentinel Lymph Node Biopsy MeSH
• Adult MeSH
• Keratin-19 genetics   MeSH
• Middle Aged MeSH
• Humans MeSH
• Lymphatic Metastasis MeSH
• Neoplasm Micrometastasis MeSH
• Survival Rate MeSH
• Biomarkers, Tumor genetics   MeSH
• Endometrial Neoplasms genetics   pathology   surgery   MeSH
• Follow-Up Studies MeSH
• Nucleic Acids analysis   genetics   MeSH
• Intraoperative Period MeSH
• Prognosis MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Sentinel Lymph Node pathology   surgery   MeSH
• Cystadenocarcinoma, Serous genetics   secondary   surgery   MeSH
• Nucleic Acid Amplification Techniques methods   MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Comparative Study MeSH

AIMS: Endometrial cancer (EC) is the most common gynecological cancer worldwide and its incidence is rising. The cornerstone of its management is surgical treatment with nodal staging. A monocentric study investigating the potential of the molecular biology method of one-step nucleic acid amplification (OSNA) in sentinel lymph node (SLN) analysis was conducted at our institution between April 2016 and January 2018. Histopathological ultrastaging was used as the reference standard for SLN examination and OSNA as the index test. The aim of this study was to assess the long-term outcome of patients with discordant SLN and OSNA results. To our knowledge, this is the first study exploring this issue. METHODS AND RESULTS: Patients were followed in line with the current ESMO/ESGO/ESTRO recommendations. The institutional electronic database was retrospectively searched for patients' follow-up data from April 2016 till March 2023. Only patients who provided a written valid consent and had a positive OSNA and negative ultrastaging of their SLN analysis were included in the study. The primary endpoint was the retrospective analysis of their clinical outcome. Data from 58 patients enrolled into our previous study were reviewed and 12 discordant patients who met the inclusion criteria for this study were identified. The median follow-up was 83 months. Disease recurrence was detected in 3 (25%) patients, two of these were nodal and both patients died. One patient had a solitary lung metastasis which was surgically treated, and the patient was disease-free during the whole study period. CONCLUSION: The recurrence rate of patients included in the study was in the intermediate-high and high-risk group range, and hence, higher than expected based on ultrastaging results. Furthermore, benign epithelial inclusions do not seem to adversely affect OSNA SLN analysis in EC patients.

• MeSH
• Sentinel Lymph Node Biopsy * MeSH
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Neoplasm Recurrence, Local pathology   genetics   epidemiology   MeSH
• Lymphatic Metastasis pathology   diagnosis   MeSH
• Endometrial Neoplasms * genetics   pathology   surgery   MeSH
• Follow-Up Studies MeSH
• Retrospective Studies MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Sentinel Lymph Node * pathology   surgery   MeSH
• Neoplasm Staging * MeSH
• Nucleic Acid Amplification Techniques * methods   MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Comparative Study MeSH