Plasmodium falciparum
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Malárie je prakticky nejzávažnějším tropickým onemocněním. V České republice je možné občas diagnostikovat malárii u osob, které pobývaly v tropech a subtropech. Plasmodium falciparum je ze 4 druhů původců malárie člověka nejnebezpečnější. V roce 1999 byl na infekční klinice FN Hradec Králové hospitalizován pacient s infekcí Plasmodium falciparum po převozu ze Súdánu. Průběh onemocnění, včetně poklesu parazitémie z 10,5 %, objevení se gametocytů, vymizení plasmodií a uzdravení pacienta je popsáno a doplněno rovněž fotografickou dokumentací.
Malaria is practically the most important parasitic dinase. In the Czech Republic sometimes we can see malarik parasites from persons which travelled tropical and subtropical countries. Of all the human malaria parasites (four species), Plasmodium falciparum is the most highly pathogenic. We report a case of one patient with Plasmodium falciparum after travelling Sudan. A course of dinase, parasitemia from 10,5 % (ring forms of the young trophozoites and also gametocytes in the microphotographs of the blood smears) to the negative results after specific treatment are written.
- MeSH
- malárie krev terapie MeSH
- parazitemie parazitologie MeSH
- Plasmodium falciparum patogenita MeSH
- Check Tag
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Měření parazitémie u falciparum malárie, vyjádřené počtem asexuálních stádií v 1 μl periferní krve, dovoluje vyhodnocení kauzálního vztahu mezi infekcí a onemocněním, sledovat účinnost léčby, respektive zjistit rezistenci parazita na aplikovaný lék a určit epidemiologické riziko probíhající infekce.
Measurement of parasitaemia in falciparum malaria by counting the number of asexual parasites against the number of leucocytes in the tick blood film, provide us with relevant information allowing critically assess the causality between infection and clinical course of disease, follow up the efficacy, or resistance of particular strain to the antimalarial drug, and finally, to assess the epidemiological relevance of recent infection.
American journal of tropical medicine and hygiene ; Supplement Vol. 50. 4
[1st ed.] 74 s. : obr., tab., grafy ; 24 cm
- MeSH
- malárie prevence a kontrola MeSH
- Plasmodium falciparum MeSH
- protozoální vakcíny MeSH
- Publikační typ
- kongresy MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- urgentní lékařství
- alergologie a imunologie
- parazitologie
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Nestr. : il. ; 32 cm
Kultivace Plasmodium falciparum in vitro v patologicky změněných erytrocytech. XXX XXX XXX
- Konspekt
- Buněčná biologie. Cytologie
- NLK Obory
- mikrobiologie, lékařská mikrobiologie
- parazitologie
- infekční lékařství
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
The emergence of artemisinin (ART) resistance in Plasmodium falciparum intra-erythrocytic parasites has led to increasing treatment failure rates with first-line ART-based combination therapies in Southeast Asia. Decreased parasite susceptibility is caused by K13 mutations, which are associated clinically with delayed parasite clearance in patients and in vitro with an enhanced ability of ring-stage parasites to survive brief exposure to the active ART metabolite dihydroartemisinin. Herein, we describe a panel of K13-specific monoclonal antibodies and gene-edited parasite lines co-expressing epitope-tagged versions of K13 in trans. By applying an analytical quantitative imaging pipeline, we localize K13 to the parasite endoplasmic reticulum, Rab-positive vesicles, and sites adjacent to cytostomes. These latter structures form at the parasite plasma membrane and traffic hemoglobin to the digestive vacuole wherein artemisinin-activating heme moieties are released. We also provide evidence of K13 partially localizing near the parasite mitochondria upon treatment with dihydroartemisinin. Immunoprecipitation data generated with K13-specific monoclonal antibodies identify multiple putative K13-associated proteins, including endoplasmic reticulum-resident molecules, mitochondrial proteins, and Rab GTPases, in both K13 mutant and wild-type isogenic lines. We also find that mutant K13-mediated resistance is reversed upon co-expression of wild-type or mutant K13. These data help define the biological properties of K13 and its role in mediating P. falciparum resistance to ART treatment.
- MeSH
- antimalarika farmakologie MeSH
- artemisininy farmakologie MeSH
- léková rezistence genetika fyziologie MeSH
- lidé MeSH
- mutace MeSH
- Plasmodium falciparum genetika metabolismus MeSH
- protozoální proteiny metabolismus MeSH
- tropická malárie parazitologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Phosphoinositide lipids play key roles in a variety of processes in eukaryotic cells, but our understanding of their functions in the malaria parasite Plasmodium falciparum is still very much limited. To gain a deeper comprehension of the roles of phosphoinositides in this important pathogen, we attempted gene inactivation for 24 putative effectors of phosphoinositide metabolism. Our results reveal that 79% of the candidates are refractory to genetic deletion and are therefore potentially essential for parasite growth. Inactivation of the gene coding for a Plasmodium-specific putative phosphoinositide-binding protein, which we named PfPX1, results in a severe growth defect. We show that PfPX1 likely binds phosphatidylinositol-3-phosphate and that it localizes to the membrane of the digestive vacuole of the parasite and to vesicles filled with host cell cytosol and labeled with endocytic markers. Critically, we provide evidence that it is important in the trafficking pathway of hemoglobin from the host erythrocyte to the digestive vacuole. Finally, inactivation of PfPX1 renders parasites resistant to artemisinin, the frontline antimalarial drug. Globally, the minimal redundancy in the putative phosphoinositide proteins uncovered in our work supports that targeting this pathway has potential for antimalarial drug development. Moreover, our identification of a phosphoinositide-binding protein critical for the trafficking of hemoglobin provides key insight into this essential process. IMPORTANCE Malaria represents an enormous burden for a significant proportion of humanity, and the lack of vaccines and problems with drug resistance to all antimalarials demonstrate the need to develop new therapeutics. Inhibitors of phosphoinositide metabolism are currently being developed as antimalarials but our understanding of this biological pathway is incomplete. The malaria parasite lives inside human red blood cells where it imports hemoglobin to cover some of its nutritional needs. In this work, we have identified a phosphoinositide-binding protein that is important for the transport of hemoglobin in the parasite. Inactivation of this protein decreases the ability of the parasite to proliferate. Our results have therefore identified a potential new target for antimalarial development.
- MeSH
- antimalarika * farmakologie MeSH
- erytrocyty parazitologie MeSH
- fosfatidylinositoly metabolismus MeSH
- hemoglobiny metabolismus MeSH
- lidé MeSH
- malárie MeSH
- paraziti metabolismus MeSH
- Plasmodium falciparum * genetika MeSH
- protozoální proteiny * genetika MeSH
- transportní proteiny metabolismus MeSH
- tropická malárie * genetika parazitologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
... Plasmodium genome papers - complete genome sequences of two plasmodium parasites and their proteomic ...
Nature
1 elektronický optický disk (CD-ROM) : barev. ; 13 cm
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- biologie
- parazitologie
- cestovní a tropická medicína
- NLK Publikační typ
- CD-ROM
