• MeSH
• galium MeSH
• nukleární lékařství MeSH
• teranostická nanomedicína MeSH
• Publikační typ
• abstrakty MeSH
• kongresy MeSH
• sborníky MeSH

• Konspekt
• Lékařské vědy. Lékařství
• NLK Obory
• radiologie, nukleární medicína a zobrazovací metody

• MeSH
• molekulární zobrazování MeSH
• nanomedicína MeSH
• nanotechnologie MeSH

• Konspekt
• Biochemie. Molekulární biologie. Biofyzika
• NLK Obory
• biologie
• genetika, lékařská genetika
• radiologie, nukleární medicína a zobrazovací metody
• NLK Publikační typ
• elektronické časopisy

• MeSH
• teranostická nanomedicína MeSH
• Publikační typ
• sborníky MeSH

• Konspekt
• Lékařské vědy. Lékařství
• NLK Obory
• radiologie, nukleární medicína a zobrazovací metody

Colorectal cancer (CRC) is the third most common cancer worldwide, and metastatic CRC is a fatal disease. The CRC-affected tissues show several molecular markers that could be used as a fresh strategy to create newer methods of treating the condition. The liver and the peritoneum are where metastasis occurs most frequently. Once the tumor has metastasized to the liver, peritoneal carcinomatosis is frequently regarded as the disease's final stage. However, nearly 50% of CRC patients with peritoneal carcinomatosis do not have liver metastases. New diagnostic and therapeutic approaches must be developed due to the disease's poor response to present treatment choices in advanced stages and the necessity of an accurate diagnosis in the early stages. Many unique and amazing nanomaterials with promise for both diagnosis and treatment may be found in nanotechnology. Numerous nanomaterials and nanoformulations, including carbon nanotubes, dendrimers, liposomes, silica nanoparticles, gold nanoparticles, metal-organic frameworks, core-shell polymeric nano-formulations, and nano-emulsion systems, among others, can be used for targeted anticancer drug delivery and diagnostic purposes in CRC. Theranostic approaches combined with nanomedicine have been proposed as a revolutionary approach to improve CRC detection and treatment. This review highlights recent studies, potential, and challenges for the development of nanoplatforms for the detection and treatment of CRC.

• MeSH
• individualizovaná medicína MeSH
• kolorektální nádory * diagnóza   farmakoterapie   MeSH
• kovové nanočástice * MeSH
• lidé MeSH
• nanočástice * terapeutické užití   MeSH
• nanomedicína metody   MeSH
• nanotrubičky uhlíkové * MeSH
• peritoneální nádory * MeSH
• systémy cílené aplikace léků metody   MeSH
• teranostická nanomedicína MeSH
• zlato MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

As a natural polysaccharide polymer, glycogen possesses suitable properties for use as a nanoparticle carrier in cancer theranostics. Not only it is inherently biocompatible, it can also be easily chemically modified with various moieties. Synthetic glycogen conjugates can passively accumulate in tumours due to enhanced permeability of tumour vessels and limited lymphatic drainage (the EPR effect). For this study, we developed and examined a glycogen-based carrier containing a gadolinium chelate and near-infrared fluorescent dye. Our aim was to monitor biodistribution and accumulation in tumour-bearing rats using magnetic resonance and fluorescence imaging. Our data clearly show that these conjugates possess suitable imaging and tumour-targeting properties, and are safe under both in vitro and in vivo conditions. Additional modification of glycogen polymers with poly(2-alkyl-2-oxazolines) led to a reduction in the elimination rate and lower uptake in internal organs (lower whole-body background: 45% and 27% lower MRI signals of oxazoline-based conjugates in the liver and kidneys, respectively compared to the unmodified version). Our results highlight the potential of multimodal glycogen-based nanopolymers as a carrier for drug delivery systems in tumour diagnosis and treatment.

• MeSH
• antitumorózní látky aplikace a dávkování   MeSH
• glykogen aplikace a dávkování   MeSH
• krysa rodu rattus MeSH
• nádorové buněčné linie MeSH
• nádory farmakoterapie   MeSH
• systémy cílené aplikace léků * MeSH
• teranostická nanomedicína * MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• lidé MeSH
• magnetická rezonanční tomografie * metody   MeSH
• nádory diagnostické zobrazování   terapie   MeSH
• teranostická nanomedicína * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• dopisy MeSH
• kongresy MeSH
• úvodníky MeSH

Here, we describe a novel polymer platform suitable for efficient diagnostics and potential theranostics based on 89Zr-labeled N-(2-hydroxypropyl)methacrylamide (HPMA)-based copolymer conjugates. A set of polymers differing in molecular weight with either low dispersity or high dispersity were designed and synthesized and their biodistribution in vivo was successfully and precisely observed over 72 h. Moreover, the feasibility of two imaging techniques, fluorescence imaging (FI) and positron emission tomography (PET), was compared using labeled polymer conjugates. Both methods gave comparable results thus showing the enhanced diagnostic potential of the prepared polymer-dye or polymer-chelator-89Zr constructs. The in vivo and ex vivo PET/FI studies indicated that the dispersity and molecular weight of the linear HPMA polymers have a significant influence on the pharmacokinetics of the polymer conjugates. The higher molecular weight and narrower distribution of molecular weights of the polymer carriers improve their pharmacokinetic profile for highly prolonged blood circulation and enhanced tumor uptake. Moreover, the same polymer carrier with the anticancer drug doxorubicin bound by a pH-sensitive hydrazone bond showed higher cytotoxicity and cellular uptake in vitro. Therefore, HPMA copolymers with low dispersity and a molecular weight near the limit of renal filtration can be used as highly efficient polymer carriers of tumor-targeted therapeutics or for theranostics with minimal side effects.

• MeSH
• doxorubicin aplikace a dávkování   farmakokinetika   MeSH
• experimentální nádory * diagnostické zobrazování   farmakoterapie   MeSH
• Jurkat buňky MeSH
• lidé MeSH
• MFC-7 buňky MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• nádorové buněčné linie MeSH
• nosiče léků * chemie   MeSH
• optické zobrazování * MeSH
• polymery * chemie   MeSH
• pozitronová emisní tomografie * MeSH
• radionuklidy MeSH
• teranostická nanomedicína * MeSH
• tkáňová distribuce MeSH
• zirkonium MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH

Traditional thrombolytic therapeutics for vascular blockage are affected by their limited penetration into thrombi, associated off-target side effects, and low bioavailability, leading to insufficient thrombolytic efficacy. It is hypothesized that these limitations can be overcome by the precisely controlled and targeted delivery of thrombolytic therapeutics. A theranostic platform is developed that is biocompatible, fluorescent, magnetic, and well-characterized, with multiple targeting modes. This multimodal theranostic system can be remotely visualized and magnetically guided toward thrombi, noninvasively irradiated by near-infrared (NIR) phototherapies, and remotely activated by actuated magnets for additional mechanical therapy. Magnetic guidance can also improve the penetration of nanomedicines into thrombi. In a mouse model of thrombosis, the thrombosis residues are reduced by ≈80% and with no risk of side effects or of secondary embolization. This strategy not only enables the progression of thrombolysis but also accelerates the lysis rate, thereby facilitating its prospective use in time-critical thrombolytic treatment.

• MeSH
• fibrinolytika chemie   terapeutické užití   MeSH
• individualizovaná medicína MeSH
• magnetické jevy MeSH
• myši MeSH
• trombolytická terapie * MeSH
• trombóza * diagnostické zobrazování   farmakoterapie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Magnetic nanoparticles offer multiple possibilities for biomedical applications. Besides their physico-chemical properties, nanoparticle-cellular interactions are determinant for biological safety. In this work, magnetic nanoparticles were synthesized by one-shot precipitation or two-step reaction and coated with biocompatible polymers, such as poly(l-lysine) and poly(N,N-dimethylacrylamide-co-acrylic acid), and carbohydrates, like l-ascorbic acid, d-galactose, d-mannose, and sucrose. The resulting magnetic nanoparticles were characterized by dynamic light scattering, FT-Raman spectroscopy, transmission electron microscopy, SQUID magnetometry, and Mössbauer spectroscopy. Ability of the nanoparticles to be used in theranostic applications was also evaluated, showing that coating with biocompatible polymers increased the heating efficiency. Nanoparticles synthesized by one-shot precipitation were 50% larger (∼13nm) than those obtained by a two-step reaction (∼8nm). Magnetic nanoparticles at concentrations up to 500μgmL-1 were non-cytotoxic to L929 fibroblasts. Particles synthesized by one-shot precipitation had little effect on viability, cell cycle and apoptosis of the three human colon cancer cell lines used: Caco-2, HT-29, and SW-480. At the same concentration (500μgmL-1), magnetic particles prepared by a two-step reaction reduced colon cancer cell viability by 20%, affecting cell cycle and inducing cell apoptosis. Uptake of surface-coated magnetic nanoparticles by colon cancer cells was dependent on particle synthesis, surface coating and incubation time.

• MeSH
• apoptóza účinky léků   MeSH
• biokompatibilní potahované materiály chemie   farmakokinetika   farmakologie   MeSH
• buněčný cyklus účinky léků   MeSH
• buňky HT-29 MeSH
• Caco-2 buňky MeSH
• lidé MeSH
• magnetické nanočástice chemie   MeSH
• magnetismus * MeSH
• myši MeSH
• nádorové buněčné linie MeSH
• nádory tračníku metabolismus   patologie   MeSH
• polymery chemie   MeSH
• povrchové vlastnosti MeSH
• teranostická nanomedicína metody   MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

The cellular adhesion receptor αvβ6-integrin is highly expressed in many cancers, e.g., pancreatic, lung, head-and-neck, cervical, bladder, and esophageal carcinoma. Multimerization of αvβ6-integrin-specific RGD peptides increases the target affinity and retention but affects biodistribution and pharmacokinetics. Amide formation of the terminal carboxylic acid moieties of the square-symmetrical bifunctional chelator DOTPI with 3-azidopropylamine yields derivatives with 4, 3, and 2 terminal azides and zero, 1, and 2 remaining carboxylic acids, respectively, whereby formation of the 2-cis-isomer is preferred according to NMR investigation of the Eu(III)-complexes. Cu(II)-catalyzed alkyne-azide cycloaddition (CuAAC) of the alkyne-functionalized αvβ6-integrin binding peptide cyclo[YRGDLAYp(NMe)K(pent-4-ynoic amide)] (Tyr2) yields the respective di-, tri-, and tetrameric conjugates for Lu-177-labeling. In mice bearing αvβ6-integrin-expressing xenografts of H2009 (human lung adenocarcinoma) cells, the Lu-177-labeled trimer's tumor-to-blood ratio of 112 exceeds that of the tetramer (10.4) and the dimer (54). Co-infusion of gelofusine (succinylated gelatin) reduces the renal uptake of the trimer by 89%, resulting in a 10-fold better tumor-to-kidney ratio, while no improvement of that ratio is observed with arginine/lysine, para-aminohippuric acid (PAH), and hydroxyethyl starch (HES) coinfusions. Since the Lu-177-labeled Tyr2-trimer outperforms the dimer and the tetramer, such trimers are considered the best lead structures for the ongoing development of αvβ6-integrin targeted anticancer theranostics.

• MeSH
• antigeny nádorové * metabolismus   MeSH
• chelátory * chemie   MeSH
• integriny * metabolismus   MeSH
• lidé MeSH
• lutecium * chemie   MeSH
• myši nahé MeSH
• myši MeSH
• nádorové buněčné linie MeSH
• nádory farmakoterapie   MeSH
• oligopeptidy * chemie   farmakokinetika   MeSH
• radiofarmaka farmakokinetika   chemie   terapeutické užití   MeSH
• radionuklidy * chemie   MeSH
• syntetická chemie okamžité shody MeSH
• teranostická nanomedicína metody   MeSH
• tkáňová distribuce MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH