boiled egg
Dotaz
Zobrazit nápovědu
BACKGROUND AND AIMS: The egg protein ovalbumin (OVA) belongs to six most frequent food allergens. We investigated how thermal processing influences its ability to induce allergic symptoms and immune responses in mouse model of food allergy. METHODOLOGY/PRINCIPAL FINDINGS: Effect of increased temperature (70°C and 95°C) on OVA secondary structure was characterized by circular dichroism and by the kinetics of pepsin digestion with subsequent HPLC. BALB/c mice were sensitized intraperitoneally and challenged with repeated gavages of OVA or OVA heated to 70°C (h-OVA). Levels of allergen-specific serum antibodies were determined by ELISA (IgA and IgGs) or by β-hexosaminidase release test (IgE). Specific activities of digestive enzymes were determined in brush border membrane vesicles of jejunal enterocytes. Cytokine production and changes in regulatory T cells in mesenteric lymph nodes and spleen were assessed by ELISA and FACS. Heating of OVA to 70°C caused mild irreversible changes in secondary structure compared to boiling to 95°C (b-OVA), but both OVA treatments led to markedly different digestion kinetics and Tregs induction ability in vitro, compared to native OVA. Heating of OVA significantly decreased clinical symptoms (allergic diarrhea) and immune allergic response on the level of IgE, IL-4, IL-5, IL-13. Furthermore, h-OVA induced lower activities of serum mast cell protease-1 and enterocyte brush border membrane alkaline phosphatase as compared to native OVA. On the other hand h-OVA stimulated higher IgG2a in sera and IFN-γ secretion by splenocytes. CONCLUSIONS: Minor irreversible changes in OVA secondary structure caused by thermal processing changes both its digestion and antigenic epitopes formation, which leads to activation of different T cell subpopulations, induces shift towards Th1 response and ultimately reduces its allergenicity.
- MeSH
- cytokiny krev imunologie MeSH
- imunoglobulin E krev imunologie MeSH
- modely nemocí na zvířatech MeSH
- myši MeSH
- ovalbumin chemie imunologie MeSH
- potravinová alergie krev imunologie MeSH
- prezentace antigenu imunologie MeSH
- regulační T-lymfocyty imunologie MeSH
- sekundární struktura proteinů MeSH
- vysoká teplota MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
... Proteins of Cells and Viruses 200 Dry Heat Requires More Time than Wet Heat to Kill Microorganisms 200 Boiling ... ... Questions 202 Using an Autoclave 202 -- Pasteurization Involves Controlled Heating at Temperatures Below Boiling ... ... Sterilization by Filtration 203 -- Perspective 9.1: Some Materials Can Be Sterilized by Temperatures Below Boiling ... ... Diagnosis of Salmonellosis 522 -- Perspective 23.3: Salmonellosis Due to Salmonella enteritidis in Raw Eggs ...
XXVIII, 812 s. : il. ; 32 cm
... [one\'s ~] for give up (~) hope go (~) out (for) lose (~) trace of pull out the stops put ~ one\'s eggs ... ... ^ bars be behind ^ put behind ~ base get to first \'v, with bash have a ~ at basket put all one\'s eggs ... ... /punch in blue be out of the ~ come out of the ^ boat push the ~ out body keep ~ and soul together boil ... ... make ~/good use of put to ~/good use efforts concentrate [one\'s (on/upon) focus [(one\'s) ~] (on) eggs ... ... [^/ a fart] put ^ across (sb): put across2 put ^ foot before/in front of the other put all one\'s eggs ...
2. vyd. 396 s.
... over my dead ~ bogey lay a/one\'s ~ : lay a/ one\'s ghost etc boggles one\'s/the mind/ imagination ~ boiling ... ... keep the pot ~1,2 boils one\'s blood ~ a watched kettle/pot never ~ bold (as) ~ as brass (as) ~/brave ... ... me breakfast a dog\'s ~/dinner eat sb alive/for ~ breaking (one can\'t) make an omelette without ~ eggs ... ... \'twixt (the) ~ and (the) lip cupboard a skeleton in the ~ cups in one\'s ~ curate\'s (like) the ~ egg ...
2. vyd. 685 s.
