celery
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The native dimeric Petroselinum crispum (Mill.) Fuss protein Pet c 1.0201 and a monomeric xyloglucan endotransglycosylase enzyme (Garajova et al., 2008) isolated from the root cells co-purify and share similar molecular masses and acidic isoelectric points. In this work, we determined the complete primary structure of the parsley Pet c 1.0201 protein, based on tryptic and chymotryptic peptides followed by the manual micro-gradient chromatographic separation coupled with offline MALDI-TOF/TOF mass spectrometry. The bioinformatics approach enabled us to include the parsley protein into the PR-10 family, as it exhibited the highest protein sequence identity with the Apium graveolens Api g 1.0201 allergen and the major Daucus carota allergen Dau c 1.0201. Hence, we designated the Petroselinum crispum protein as Pet c 1.0201 and deposited it in the UniProt Knowledgebase under the accession C0HKF5. 3D protein homology modelling and molecular dynamics simulations of the Pet c 1.0201 dimer confirmed the typical structure of the Bet v 1 family allergens, and the potential of the Pet c 1.0201 protein to dimerize in water. However, the behavioural properties of Pet c 1.0201 and the celery allergen Api g 1.0101 differed in the presence of salts due to transiently and stably formed dimeric forms of Pet c 1.0201 and Api g 1.0101, respectively.
- MeSH
- alergeny MeSH
- Apium * MeSH
- mrkev obecná * MeSH
- petržel (rod) MeSH
- rostlinné proteiny MeSH
- Publikační typ
- časopisecké články MeSH
Water celery (Helosciadium nodiflorum, Apiaceae) is a common and invasive hydrophyte occurring in rivers and slow waters of Europe. In the past, it was consumed as a vegetable and spices, but currently it has mostly been abandoned. As a result, this plant produces an unexploited biomass occluding rivers and slow waters. With the aim to valorise water celery as an important biomass source for the development of botanical insecticides, in this work, we investigated the inhibitory effects of the essential oil hydrodistilled from its aerial parts against larvae of the filariasis vector Culex quinquefasciatus, adults of the housefly Musca domestica, and larvae of the African cotton leafworm Spodoptera littoralis. The essential oil, which was characterized by the insecticidal limonene (39.8%) and myristicin (35.3%), exerted important toxic effects on 3rd instar larvae of C. quinquefasciatus and S. littoralis and on adults of M. domestica, with LD50of 50.4 μl l-1, 116.7 μg larva-1, and 45.1 (male) and 70.1 (female) μg adult-1, respectively. The oil did not show any noticeable inhibitory activity on the acetylcholinesterase enzyme. Our findings add scientific evidences highlighting water celery as an important biomass source to be used for the control of insect pests and vectors of high economic importance.
- MeSH
- acetylcholinesterasa chemie metabolismus MeSH
- Apiaceae chemie metabolismus MeSH
- Culex účinky léků růst a vývoj MeSH
- insekticidy chemie toxicita MeSH
- larva účinky léků MeSH
- LD50 MeSH
- moucha domácí účinky léků růst a vývoj MeSH
- oleje prchavé analýza chemie farmakologie MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- Spodoptera účinky léků růst a vývoj MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Autoři popisují kazuistiku pacientky s přecitlivělostí na celer, u které byla k diagnostice využita nová laboratorní metoda – test aktivace bazofilů. Tento test byl schopen přesněji identifikovat alergen odpovědný za klinické příznaky časné přecitlivělosti než v současnosti běžně používaná diagnostická vyšetření.
The authors describe case report of patient with allergy to cellery. New laboratory method – the flow cytometric basophil activation test was used for diagnosis. This test identified the allergen responsible for clinical symptoms of immediate type of senzitization more precisely than contemporary routinely used diagnostic tests.
The aim of this study was to assess the effect of a complete nitrite replacement strategy using celery, carmine, sodium lactate and orange dietary fibre combined with vitamins C and E, on the quality characteristics (technological, sensorial and safety properties) of hot dog sausages (five samples) during chilled storage (2 ± 1℃ 60 days). Nitrite replacers (combined with vitamins C and E) presented antioxidant activity, reducing lipid oxidation in reformulated samples. At the end of storage redness (a*) was similar in the control sample (with added nitrite) and in the sample without added nitrite. Sensory evaluation detected no significant difference between samples with and without added nitrite. All the reformulated samples were judged acceptable by the panellists. At the end of storage, the control sample contained more than four times as much residual nitrite as the reformulated samples. Growth of presumptive Clostridium perfringens was not observed in any of the samples. Samples without added nitrite had longer shelf-lives than control sausage. Samples containing 0.1% vitamin C registered the lowest microbiological levels. This strategy could be a good alternative to reduce and/or eliminate added nitrite in hot dog sausages.
- MeSH
- antiinfekční látky farmakologie MeSH
- antioxidancia farmakologie MeSH
- Apium MeSH
- chuť * MeSH
- Citrus chemie MeSH
- Clostridium perfringens účinky léků růst a vývoj MeSH
- dusitany * MeSH
- karmín farmakologie MeSH
- konzervace potravin metody MeSH
- kyselina askorbová farmakologie MeSH
- lidé MeSH
- manipulace s potravinami metody MeSH
- masné výrobky analýza mikrobiologie MeSH
- natriumlaktát farmakologie MeSH
- peroxidace lipidů účinky léků MeSH
- potravinářská mikrobiologie MeSH
- potravní vláknina MeSH
- skladování potravin metody MeSH
- skot MeSH
- vitamin E farmakologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
