The integrated stress response is able to rapidly shut down the synthesis of proteins in eukaryotic cells.
Histologically verified pairs (n=10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw C{q} values correlated with the degree of RNA degradation. This correlation was abolished by normalization to C{q} of 18S endogenous control gene. Both geNorm and NormFinder programs suggested EIF2B1, ELF1, MRPL19, and POP4 as the same most stable genes. We have thus identified suitable reference genes for future expression studies in pancreatic carcinoma. Normalization method reducing the effects of RNA degradation on the quality of results was also developed.
- MeSH
- Eukaryotic Initiation Factor-2B genetics MeSH
- Gene Expression MeSH
- Nuclear Proteins genetics MeSH
- Carcinoma diagnosis genetics pathology MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Middle Aged MeSH
- Humans MeSH
- Mitochondrial Proteins genetics MeSH
- Biomarkers, Tumor genetics MeSH
- Pancreatic Neoplasms diagnosis genetics pathology MeSH
- Pancreas metabolism pathology MeSH
- Ribonucleases genetics MeSH
- Ribonucleoproteins genetics MeSH
- Ribosomal Proteins genetics MeSH
- Aged MeSH
- Software MeSH
- RNA Stability MeSH
- Gene Expression Profiling MeSH
- Transcription Factors genetics MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Eukaryotic Initiation Factor-2B physiology MeSH
- Research Support as Topic MeSH
- Phosphatidylinositol 3-Kinases metabolism MeSH
- Phosphoinositide-3 Kinase Inhibitors MeSH
- Rabbits MeSH
- Proto-Oncogene Proteins metabolism MeSH
- Avian Sarcoma Viruses pathogenicity MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
