halogenated
Dotaz
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IARC monographs on the evaluation of carcinogenic risk of chemicals to humans ; Vol. 20
609 s. : tab.
- MeSH
- halogenované uhlovodíky MeSH
- karcinogeny MeSH
- pesticidy MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Chemie. Mineralogické vědy
- NLK Obory
- chemie, klinická chemie
- toxikologie
- onkologie
IARC monographs on the evaluation of carcinogenic risk of chemicals to human ; Vol. 41
434 s. : tab.
396 s. : il.
An enzyme-based biosensor was developed by co-immobilization of purified enzyme haloalkane dehalogenase (EC 3.8.1.5) and a fluorescence pH indicator on the tip of an optical fiber. Haloalkane dehalogenase catalyzes hydrolytic dehalogenation of halogenated aliphatic hydrocarbons, which is accompanied by a pH change influencing the fluorescence of the indicator. The pH sensitivity of several fluorescent dyes was evaluated. The selected indicator 5(6)-carboxyfluorescein was conjugated with bovine serum albumin and its reaction was tested under different immobilization conditions. The biosensor was prepared by cross-linking of the conjugate in tandem with haloalkane dehalogenase using glutaraldehyde vapor. The biosensor, stored for 24 h in 50 mM phosphate buffer (pH 7.5) prior to measurement, was used after 15 min of equilibration, the halogenated compound was added, and the response was monitored for 30 min. Calibration of the biosensor with 1,2-dibromoethane and 3-chloro-2-(chloromethyl)-1-propene showed an excellent linear dependence, with detection limits of 0.133 and 0.014 mM, respectively. This biosensor provides a new tool for continuous in situ monitoring of halogenated environmental pollutants.
- MeSH
- biosenzitivní techniky přístrojové vybavení metody MeSH
- enzymy imobilizované chemie MeSH
- fluorescenční barviva chemie MeSH
- halogenované uhlovodíky analýza chemie MeSH
- molekulární struktura MeSH
- skot MeSH
- technologie optických vláken MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
