This study presents an innovative multifunctional system in fabricating new functional wound dressing (FWD) products that could be used for skin regeneration, especially in cases of infected chronic wounds and ulcers. The innovation is based on the extraction, characterization, and application of collagen (CO)/chitosan-glucan complex hollow fibers (CSGC)/aloe vera (AV) as a novel FWS. For the first time, specific hollow fibers were extracted with controlled inner (500-900 nm)/outer (2-3 µm) diameters from mycelium of Schizophyllum commune. Further on, research and evaluation of morphology, hydrolytic stability, and swelling characteristics of CO/CSGC@AV were carried out. The obtained FWS showed high hydrolytic stability with enhanced swelling characteristics compared to native collagen. The hemostatic effect of FWS increased significantly in the presence of CSGC, compared to native CO and displayed excellent biocompatibility which was tested by using normal human dermal fibroblast (NHDF). The FWS showed high antibacterial activity against different types of bacteria (positive/negative grams). From in vivo measurements, the novel FWS increased the percentage of wound closure after one week of treatment. All these results imply that the new CO/CSGC@AV-FWD has the potential for clinical skin regeneration and applying for controlled drug release.

• MeSH
• Aloe * chemistry   MeSH
• Anti-Bacterial Agents chemistry   isolation & purification   pharmacology   MeSH
• Bacteria drug effects   growth & development   MeSH
• Chitosan chemistry   isolation & purification   pharmacology   MeSH
• Fibrillar Collagens chemistry   isolation & purification   pharmacology   MeSH
• Glucans chemistry   isolation & purification   pharmacology   MeSH
• Wound Healing drug effects   MeSH
• Cells, Cultured MeSH
• Skin drug effects   injuries   pathology   MeSH
• Humans MeSH
• Disease Models, Animal MeSH
• Mycelium * chemistry   MeSH
• Bandages * MeSH
• Rats, Wistar MeSH
• Wounds and Injuries drug therapy   pathology   MeSH
• Plant Extracts chemistry   isolation & purification   pharmacology   MeSH
• Schizophyllum * chemistry   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Comparative Study MeSH

The proposed project is concerned with the design and fabrication of new types of structured drug carriers called multifuctional nanoclusters. A nanocluster consists of primary particles in the size range from 1’s to 10’s nm, and the cluster itself will have typical dimensions in the 100’s nm. Thank to their modular composition, the nanoclusters will act not only as drug carriers able to combine several active components of different nature (hydrophilic, hydrophobic, peptides, nucleic acids) but also as diagnostic agents (e.g. for magnetic resonance imaging). We will develop and validate technologies for the fabrication of nanoclusters and carry out a systematic study of the relationship between composition, fabrication conditions, and final properties, which will be tested not only using static cell cultures, but also in 3D perfusion cell cultures that simulate the flow of physiological fluids. The project will open up new opportunities for the acceleration of the development of modern drug carriers and their reliable and scalable fabrication.

Navrhovaný projekt se týká přípravy nového typu strukturovaných nosičů léčiv – tzv. multifunkčních nanoclusterů. Nanocluster sestává z primárních částic o velikosti v řádu jednotek až desítek nanometrů, samotný nanocluster pak bude dosahovat typických rozměrů v řádu stovek nanometrů. Díky svému modulárnímu složení budou nanoclustry sloužit nejen jako nosiče léčiv umožňující kombinaci několika účinných látek odlišného charakteru (např. hydrofilní, hydrofobní, peptidy, nukleové kyseliny), ale mohou též plnit funkci diagnostickou (např. jako kontrastní látka pro zobrazovací metody). Budou vyvinuty a ověřeny technologie přípravy nanoclusterů, založené na řízené agregaci nanočástic, dále bude provedena systematická studie vztahu mezi složením, podmínkami přípravy a výslednými vlastnostmi, které budou testovány jak na statických buněčných kulturách, tak za průtočných podmínek simulujících tok fyziologických tekutin. Projekt otevře nové možnosti pro zrychlení vývoje moderních nosičů léčiv a jejich spolehlivé a škálovatelné přípravy.

• MeSH
• Molecular Targeted Therapy methods   MeSH
• Delayed-Action Preparations therapeutic use   MeSH
• Nanoparticles therapeutic use   MeSH
• Antineoplastic Agents MeSH
• Drug Development MeSH

• Conspectus
• Farmacie. Farmakologie
• NML Fields
• farmacie a farmakologie
• farmakoterapie
• NML Publication type
• závěrečné zprávy o řešení grantu AZV MZ ČR

Emergent application of antimicrobial strategies as symptomatic treatment in coronavirus disease (COVID-19) and linkage of severe acute respiratory syndrome coronavirus2 with microbial infections, has created colossal demand for antimicrobials. For the first time, this communication explore the physicochemical, antifungal, antibacterial, and photocatalytic properties of biogenic magnesium nanoparticles (MgNPs), synthesized using essential oil of Cymbopogon flexuosus's as an efficient multifunctional reducing and stabilizing/capping reagent. It is observed that MgNPs (ranging in size: 8-16 nm) of varying phytochemical compositions (MgS1, MgS2, MgS3) exhibited various useful physicochemical, antimicrobial, and photocatalytic properties. FTIR outcomes highlight the functional biomolecules-assisted reduction of Mg from Mg+ to Mg0. Among all, MgS3-Nps owing to the smallest particle size exhibited superior photocatalytic efficacy (91.2%) for the methylene blue degradation upon direct exposure to the sunlight for 3 h without using any reducing agents. Fabricated MgNPs also exhibited excellent antifungal (against Fusarium oxysporum) and antibacterial (versus Staphylococcus aureus and Escherichia coli) efficacies compared to state-of-the-art antimicrobial agents deployed for the treatment of infectious diseases. Based on this investigated greener approach, imperative from economic and environmental viewpoint, such essential oil based-MgNPs can be a potential nanosystem for various industrial applications where photocatalytic, and biomedical attributes are the key requirements.

• MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• Antifungal Agents pharmacology   MeSH
• COVID-19 * MeSH
• Escherichia coli MeSH
• COVID-19 Drug Treatment MeSH
• Magnesium MeSH
• Humans MeSH
• Nanoparticles * MeSH
• Oils, Volatile * pharmacology   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Chemotherapy is the most prominent route in cancer therapy for prolonging the lifespan of cancer patients. However, its non-target specificity and the resulting off-target cytotoxicities have been reported. Recent in vitro and in vivo studies using magnetic nanocomposites (MNCs) for magnetothermal chemotherapy may potentially improve the therapeutic outcome by increasing the target selectivity. In this review, magnetic hyperthermia therapy and magnetic targeting using drug-loaded MNCs are revisited, focusing on magnetism, the fabrication and structures of magnetic nanoparticles, surface modifications, biocompatible coating, shape, size, and other important physicochemical properties of MNCs, along with the parameters of the hyperthermia therapy and external magnetic field. Due to the limited drug-loading capacity and low biocompatibility, the use of magnetic nanoparticles (MNPs) as drug delivery system has lost traction. In contrast, MNCs show higher biocompatibility, multifunctional physicochemical properties, high drug encapsulation, and multi-stages of controlled release for localized synergistic chemo-thermotherapy. Further, combining various forms of magnetic cores and pH-sensitive coating agents can generate a more robust pH, magneto, and thermo-responsive drug delivery system. Thus, MNCs are ideal candidate as smart and remotely guided drug delivery system due to a) their magneto effects and guide-ability by the external magnetic fields, b) on-demand drug release performance, and c) thermo-chemosensitization under an applied alternating magnetic field where the tumor is selectively incinerated without harming surrounding non-tumor tissues. Given the important effects of synthesis methods, surface modifications, and coating of MNCs on their anticancer properties, we reviewed the most recent studies on magnetic hyperthermia, targeted drug delivery systems in cancer therapy, and magnetothermal chemotherapy to provide insights on the current development of MNC-based anticancer nanocarrier.

• MeSH
• Hyperthermia, Induced * methods   MeSH
• Drug Delivery Systems methods   MeSH
• Humans MeSH
• Magnetic Fields MeSH
• Magnetics MeSH
• Neoplasms * drug therapy   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Review MeSH