Ischemicko-reperfuzní procesy provázející akutní koronární syndromy jsou provázeny významnou aktivací imunitního systému. Molekula CD163 patří mezi významné molekuly scavengerového systému, které se podílejí na eliminaci hemoglobin-haptoglobinového komplexu a mají tak potenciál snížit produkci reaktivních kyslíkových radikálů. Cílem studie bylo sledovat změny hladin sCD163 v průběhu 96 hodin akutního koronárního syndromu léčeného direktní perkutánní intervencí. V souboru 29 pacientů (25 mužů, 4 žen) průměrného věku 64,55 let ± 7,55 let byly stanoveny hladiny sCD163 v době přijetí, následně za 24 a 96 hodin. Studie prokázala významné zvýšení hladiny sCD163 již v čase přijetí a v dalších měřeních se dále zvyšovala [čas 0 hod: 1770,5 (1451; 2096,25) vs. 1178 (1078; 1265) μg/l, p < 0,001, 24 hod.: 2063 (1589; 2453) vs. 1178 (1078; 1265) μg/l, p < 0,001, 96 hod: 2116 (1924; 2458) vs. 1178 (1078; 1265) μg/l, p < 0,001]. Analýza vztahu k funkci levé komory (ejekční frakci) prokázala statisticky významnou asociaci ve všech sledovaných obdobích. Studie ukázala na potenciální význam sCD163 pro stratifikaci rizika těchto nemocných.

Background: The ischemia/reperfusion process in the course of acute coronary syndrome with ST-segment elevation is associatedwith significant increase of immune system activation. CD163 molecule belongs to important parts of the scavenger system,which helps to eliminate hemoglobin-haptoglobin complex and has the potential to suppress reactive oxygen radicals production. Aim: The aim of our study was to assess sCD163 level in the course of STEMI.Patients and method: Plasma sDC163 level was assessed in a group of 29 patients (25 males, 4 females) of average age 64.5 let± 7.5 yrs. at the time of admission, at 24 hours and at 96 hours. Results: The plasma sCD163 level was increasing during the time of observation [0h: 1770.5 (1451; 2096.25) μg/L vs. 1178 (1078;1265) μg/L, p < 0.001, 24h: 2063 (1589; 2453) vs. 1178 (1078;1265) μg/L, p < 0.001, 96h: 2116 (1924; 2458) vs. 1178 (1078; 1265) μg/L, p < 0.001]. Plasma sCD163 level was associated with leftventricle ejection fraction. Conclusion: The study showed a potential significance of sCD163 assessment as a prognostic marker.

• MeSH
• biologické markery krev   MeSH
• CD antigeny * krev   MeSH
• humorální imunita MeSH
• infarkt myokardu s elevacemi ST úseků * MeSH
• lidé středního věku MeSH
• lidé MeSH
• reperfuze myokardu MeSH
• scavengerové receptory MeSH
• senioři MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• klinická studie MeSH
• práce podpořená grantem MeSH

BACKGROUND: CD163 is the monocyte/macrophage receptor for haptoglobin-haemoglobin complexes. The aim of this study was to assess the kinetics in the expression of CD163 on monocytes and the concentration of soluble sCD163 in serum of psoriatic patients in order to examine the effect of Goeckerman therapy. METHODS: sCD163 was measured in 71 patients before and after therapy, and in 57 healthy donors. A subgroup of 40 patients and 25 controls was used to assess the expression of membrane CD163. sCD163 was evaluated by ELISA. Flow cytometry method was used to determine the expression of membrane CD163 on monocytes, expressed as mean fluorescence index (MFI). RESULTS: Before therapy, the serum level of sCD163 was significantly higher in our patients than in controls (P=0.0154). However, we observed a profound decrease in sCD163 in our patients after therapy (P=0.0037). Similar to sCD163, pre-treatment expression of CD163 on monocytes was significantly more enhanced in patients than that in controls (P=0.0078). There was a trend towards down-regulation of the expression after therapy, nonetheless, the change was not statistically significant compared to the values before therapy (P=0.8666). This was also confirmed by comparison with controls which displayed lower expression of CD163 than patients after therapy (P=0.0019). The disease activity, expressed as PASI score, was significantly decreased in our patients by GT (P=0.0001). CONCLUSIONS: While sCD163 level in psoriatic patients was diminished after GT therapy, CD163 expression on monocytes was altered only to a minor extent.

• MeSH
• aktivace makrofágů MeSH
• antigeny diferenciační myelomonocytární krev   fyziologie   MeSH
• antigeny povrchové analýza   MeSH
• aplikace kožní MeSH
• biologické markery MeSH
• CD antigeny krev   fyziologie   MeSH
• dehet uhelný aplikace a dávkování   účinky záření   terapeutické užití   MeSH
• dospělí MeSH
• ELISA MeSH
• fotochemoterapie * MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• monocyty metabolismus   MeSH
• poškození DNA MeSH
• průtoková cytometrie MeSH
• psoriáza krev   farmakoterapie   imunologie   MeSH
• receptory buněčného povrchu krev   fyziologie   MeSH
• rozpustnost MeSH
• stupeň závažnosti nemoci MeSH
• ultrafialové záření MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

BACKGROUND: The scavenger receptor for complexes hemoglobin-haptoglobin (CD163), which is expressed on monocytes/ macrophages, is shed to the body fluids in a soluble form (sCD163). OBJECTIVES: To evaluate the dynamics of sCD163 in the blood of patients undergoing cardiac surgery. PATIENTS AND METHODS: Sixty-one adult patients who underwent coronary artery bypass grafting (CABG) were enrolled in the study. They were assigned to undergo CABG using either cardiopulmonary bypass (CPB), "on-pump", (22 patients), modified CPB, mini "on-pump", (17 patients) or without CPB, "off-pump", (22 patients) surgery. Serum levels of sCD163 in venous blood samples taken before and after surgery, and during an early postoperative period, were evaluated by Macro 163(TM) diagnostic kit (IQ Products, Groningen, NL). RESULTS: Compared to the preoperative levels ("on-pump"; 344 ng/mL, "off-pump"; 314.5 ng/mL, mini-invasive "on-pump"; 336.5 ng/mL) serum levels were elevated at the finish of surgery, reaching maximum at the 1(st) postoperative day ("onpump"; 658 ng/mL; p<0.05, "off-pump"; 810.5 ng/mL; p<0.01; mini-invasive "on-pump"; 663 ng/mL; non-significant).No significant differences regarding the serum levels of sCD163 between different surgical approaches were found. CONCLUSION: Serum level of sCD163 scavenger molecule for hemoglobin is elevated at the end of surgery and at the 1(st) postoperative day, being little influenced by cardiopulmonary bypass.

• MeSH
• antigeny diferenciační myelomonocytární krev   MeSH
• CD antigeny krev   MeSH
• haptoglobiny metabolismus   MeSH
• hemoglobiny metabolismus   MeSH
• kardiopulmonální bypass škodlivé účinky   MeSH
• koronární bypass škodlivé účinky   MeSH
• lidé středního věku MeSH
• lidé MeSH
• receptory buněčného povrchu krev   MeSH
• senioři MeSH
• zánět etiologie   krev   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH

• MeSH
• antigeny diferenciační myelomonocytární * MeSH
• lidé MeSH
• zánět * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• komentáře MeSH
• práce podpořená grantem MeSH

OBJECTIVE: To determine changes in the amniotic fluid, soluble form of scavenger receptor for hemoglobin (sCD163) concentrations during advancing gestation, and in patients with preterm premature rupture of membranes (PPROM) complicated by histological chorioamnionitis were studied. METHODS: One hundred and fifty-two women with singleton pregnancies were enrolled. The concentration of sCD163 in amniotic fluid was determined using sandwich enzyme immunoassay technique. RESULTS: Women in the midtrimester had a significantly higher median amniotic fluid sCD163 concentration than those at term not in labor (308 ng/ml vs. 217 ng/ml; p = 0.04). Patients with PPROM and histological chorioamnionitis had a higher median amniotic fluid sCD163 level than those with PPROM without histological chorioamnionitis (885 ng/ml vs. 288 ng/ml; p < 0.0001). CONCLUSIONS: Amniotic fluid sCD163 concentrations decrease with advancing gestation. Amniotic fluid sCD163 concentrations are significantly higher in women with PPROM between 24 and 36 gestational weeks with histological chorioamnionitis than those without histological signs of inflammation.

• MeSH
• antigeny diferenciační myelomonocytární metabolismus   MeSH
• biologické markery metabolismus   MeSH
• CD antigeny metabolismus   MeSH
• chorioamnionitida metabolismus   patologie   MeSH
• dospělí MeSH
• ELISA MeSH
• lidé MeSH
• plodová voda metabolismus   MeSH
• porodní děj metabolismus   MeSH
• předčasný odtok plodové vody metabolismus   patologie   MeSH
• průřezové studie MeSH
• receptory buněčného povrchu metabolismus   MeSH
• těhotenství MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

OBJECTIVE: To determine whether umbilical cord blood concentrations of soluble scavenger receptor for hemoglobin (sCD163) and pentraxin 3 (PTX3) are of value in the early postpartum diagnosis of histological chorioamnionitis in preterm prelabor rupture of membranes (PPROM). METHODS: Eighty-three women with pregnancies complicated by PPROM between 24 and 36 weeks of gestation with (n = 38) and without (n = 45) the presence of histological chorioamnionitis were included in the study. We compared umbilical cord blood sCD163 and PTX3 levels in preterm neonates from PPROM pregnancies with versus without the presence of histological chorioamnionitis using nonparametric test (Mann-Whitney U test). RESULTS: The presence of histological chorioamnionitis was associated with a higher median umbilical cord blood sCD163, but not PTX3 concentration, to compare with the absence of histological chorioamnionitis [sCD163: median 1466 ng/mL, interquartile range (IQR) 1187-1828 vs. 1168 ng/mL, IQR 887-1595; p = 0.01; PTX3: median 3.96 ng/mL, IQR 2.24-6.77 vs. 2.95 ng/mL, IQR 1.74-6.93; p = 0.49]. CONCLUSIONS: HCA is associated with an increase of umbilical cord blood sCD163, but not PTX3 concentration. Umbilical cord blood sCD163 seems to be a postpartum marker of the presence of histological chorioamnionitis.

• MeSH
• antigeny diferenciační myelomonocytární krev   MeSH
• biologické markery krev   MeSH
• C-reaktivní protein metabolismus   MeSH
• CD antigeny krev   MeSH
• chorioamnionitida krev   diagnóza   MeSH
• dospělí MeSH
• fetální krev metabolismus   MeSH
• lidé MeSH
• novorozenec nedonošený MeSH
• novorozenec MeSH
• poporodní období MeSH
• prospektivní studie MeSH
• receptory buněčného povrchu krev   MeSH
• sérový amyloidový protein metabolismus   MeSH
• těhotenství MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• novorozenec MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

CONTEXT: Soluble CD163 (sCD163) was suggested as a biomarker of insulin sensitivity and CD163 mRNA expression representing macrophage content in adipose tissue (AT). OBJECTIVE: The aim of this study was to investigate, in cross-sectional and prospective design, the relationship between sCD163 circulating levels and CD163 mRNA expression in adipose tissue and insulin sensitivity assessed by euglycemic-hyperinsulinemic clamp. DESIGN, SETTING, PARTICIPANTS, AND INTERVENTIONS: Two cohorts of subjects were examined in the study. Cohort 1 included 42 women with a wide range of body mass index (17-48 kg/m(2)); cohort 2 included 27 obese women who followed a dietary intervention consisting of 1 month of a very low-calorie diet and 5 months of a weight-stabilization period. MAIN OUTCOME MEASURES: Serum levels of CD163 and mRNA expression of CD163 and CD68 in sc and visceral (visc) AT were determined, and insulin sensitivity [expressed as glucose disposal rate (GDR)] was measured in cohort 1. In cohort 2, serum levels of CD163, mRNA expressions of CD163, CD68, and CD163-shedding factors [TNF-α-converting enzyme (TACE) and tissue inhibitor of metalloproteinase (TIMP3)] in sc AT were examined and GDR was measured before and during dietary intervention. RESULTS: In cohort 1, circulating sCD163 correlated with CD163 mRNA levels in both sc and visc AT. sCD163 and CD163 mRNA expression in both fat depots correlated with GDR. In cohort 2, the diet-induced changes of sCD163 levels did not correlate with those of CD163, CD68, TACE, and TIMP3 mRNA levels. Although the pattern of the diet-induced change of sCD163 paralleled that of GDR, there was no correlation between the changes of these two variables. CONCLUSION: sCD163 correlates with CD163 mRNA expression in sc and visc AT and with whole-body insulin sensitivity in the steady-state condition. These associations are not observed with respect to the diet-induced changes during a weight-reducing hypocaloric diet.

• MeSH
• antigeny diferenciační myelomonocytární krev   genetika   MeSH
• CD antigeny krev   genetika   MeSH
• dospělí MeSH
• glykemický clamp MeSH
• inzulinová rezistence genetika   MeSH
• kalorická restrikce * MeSH
• kohortové studie MeSH
• lidé středního věku MeSH
• lidé MeSH
• messenger RNA genetika   metabolismus   MeSH
• mladý dospělý MeSH
• obezita * dietoterapie   genetika   metabolismus   MeSH
• průřezové studie MeSH
• receptory buněčného povrchu krev   genetika   MeSH
• senioři MeSH
• tuková tkáň metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky kontrolované MeSH
• práce podpořená grantem MeSH

CD163 is a marker of macrophages with anti-inflammatory properties and its soluble form (sCD163) is considered a prognostic predictor of several diseases including type 2 diabetes mellitus (T2DM). We explored sCD163 levels at baseline and after very low-calorie diet (VLCD) or bariatric surgery in 32 patients with obesity (20 undergoing VLCD and 12 bariatric surgery), 32 obese patients with T2DM (22 undergoing VLCD and 10 bariatric surgery), and 19 control subjects. We also assessed the changes of CD163 positive cells of monocyte-macrophage lineage in peripheral blood and subcutaneous adipose tissue (SAT) in subset of patients. Plasma sCD163 levels were increased in obese and T2DM subjects relative to control subjects (467.2+/-40.2 and 513.8+/-37.0 vs. 334.4+/-24.8 ng/ml, p=0.001) and decreased after both interventions. Obesity decreased percentage of CD163+CD14+ monocytes in peripheral blood compared to controls (78.9+/-1.48 vs. 86.2+/-1.31 %, p=0.003) and bariatric surgery decreased CD163+CD14+HLA-DR+ macrophages in SAT (19.4+/-2.32 vs. 11.3+/-0.90 %, p=0.004). Our data suggest that increased basal sCD163 levels are related to obesity and its metabolic complications. On the contrary, sCD163 or CD163 positive cell changes do not precisely reflect metabolic improvements after weight loss.

• MeSH
• antigeny diferenciační myelomonocytární krev   MeSH
• bariatrická chirurgie trendy   MeSH
• biologické markery krev   MeSH
• CD antigeny krev   MeSH
• diabetes mellitus 2. typu krev   diagnóza   terapie   MeSH
• dospělí MeSH
• kalorická restrikce trendy   MeSH
• krevní glukóza metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• makrofágy metabolismus   MeSH
• obezita krev   diagnóza   terapie   MeSH
• receptory buněčného povrchu krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND: The aim of our study was to assess the impact of increased iron stores on the presence of asymptomatic atherosclerosis in a cohort of healthy men. We anticipated that higher iron stores would be associated with higher soluble cluster of differentiation 163 (sCD163) concentrations, elevated markers of oxidative stress, inflammation and higher common carotid intima-media thickness, independently of traditional risk factors of atherosclerosis. METHODS: In this cross-sectional study that included 72 healthy men, we measured the ultrasonography of common carotid intima-media thickness (IACC), the ratio of plasma-circulating transferrin receptors concentration to plasma ferritin concentration, certain inflammatory and oxidative stress markers, insulin sensitivity, plasma lipids and markers of endothelial dysfunction. RESULTS: The plasma-circulating transferrin receptor concentration to plasma ferritin concentration ratio (TfR/F) showed significant association with IACC (r=-0·310, P=0·008 vs. r=0·295, P=0·012). Multivariate analysis confirmed that the correlation of TfR/F with IACC is independent of traditional risk factors of atherosclerosis. The TfR/F ratio correlated with other indicators of atherosclerotic process fibrinogen (r=-0·292, P=0·013), von Willebrand factor (vWf; r=0·284, P=0·017), sCD163 (r=0·239, P=0·043) and IL-8 (r=0·233, P=0·049). In multivariate analysis, TfR/F independently correlated with haemoglobin (β=-0·220, P=0·047), fibrinogen (β=-0·290, P=0·009), IL-8 (β=0·227, P=0·039) and sCD163 (β=0·244, P=0·025); however, when vWf was added, significant independent correlation was seen only with fibrinogen (β=-0·301, P=0·007) and IL-8 (β=0·219, P=0·047). In addition, we demonstrated the independent correlation of sCD163 with vWf (β=0·240, P=0·040). CONCLUSIONS: Our study showed a clear association of body iron stores expressed by the TfR/F ratio with asymptomatic carotid atherosclerosis. TfR/F further exhibited an independent positive correlation with fibrinogen and a negative correlation with sCD163 and IL-8.

• MeSH
• biologické markery metabolismus   MeSH
• cévní endotel ultrasonografie   MeSH
• dospělí MeSH
• ferritin krev   MeSH
• fibrinogen metabolismus   MeSH
• interleukin-8 krev   MeSH
• intimomediální šíře tepenné stěny MeSH
• kohortové studie MeSH
• lidé středního věku MeSH
• lidé MeSH
• multivariační analýza MeSH
• nemoci arterie carotis krev   MeSH
• oxidační stres fyziologie   MeSH
• průřezové studie MeSH
• receptory transferinu krev   MeSH
• rizikové faktory MeSH
• von Willebrandův faktor metabolismus   MeSH
• železo metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

BACKGROUND: Chemical pleurodesis using videothoracoscopically applied powdered talc under general anaesthesia and selective pulmonary ventilation is the most effective method of palliative therapy for malign pleural exudate. Talc produces an intense systemic inflammatory reaction with the development of aseptic pleurisy. The result is obliteration of the pleural cavity provided there is an intense inflammatory reaction in the pleural cavity and good contact of both pleural leaves. The course of the adherence of pleural leaves can be followed using skiascopy. In routine practice, the course of local inflammatory markers is difficult to evaluate. Selection of suitable patients who will respond to this procedure is another principal obstacle of this surgical method. AIM: To evaluate the course of local inflammatory changes in the pleural cavity following application of talc and to quantify their dynamics. Selection of specific biomarkers to predict the intensity of inflammation in the pleural exudate for targeted selection of patients suitable for talcage was the second aim of this study. MATERIALS AND METHODS: 114 patients were retrospectively divided into Group A (N1 = 98) or patients without relapse and Group B (N2 = 16), patients with relapse of exudate formation. The need for repeated thoracic punctures or drainage over the course of a 12-month monitoring period was a criterion of treatment failure. Quantification of the effusion was performed by ultrasonic examination over a one year observational period at 3-monthly intervals. The concentration of soluble CD163 scavenger receptor and soluble Apo/Fas molecule was determined in exudate by ELISA. RESULTS: Soluble receptors in the 2 groups differed significantly. Group B showed higher sCD163 levels before talcage (P0 = 0.00024), faster dynamic decline in 2 h (P2 = 0.0092) and in 24 h (P24 = 0.0087). During monitoring, decrease in group B was statistically significant at 2 h (P2 = 0.056) and at 24 hrs (P24 = 0.0066). CONCLUSION: This pilot study showed that high values of sCD163 and sApo/Fas in the pleural exudate can predict treatment failure. Unsuitable candidates for this type of therapy couldbe selected in this way.

• MeSH
• biologické markery metabolismus   MeSH
• exsudáty a transsudáty metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lymfom komplikace   MeSH
• maligní pleurální výpotek etiologie   terapie   MeSH
• mastek aplikace a dávkování   škodlivé účinky   MeSH
• mezoteliom komplikace   MeSH
• nádory plic komplikace   MeSH
• paliativní péče metody   MeSH
• pleurální dutina MeSH
• pleuritida chemicky indukované   MeSH
• pleurodéza škodlivé účinky   metody   MeSH
• retrospektivní studie MeSH
• senioři MeSH
• výběr pacientů * MeSH
• výsledek terapie MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH