seeds Dotaz Zobrazit nápovědu
- MeSH
- léčivé rostliny MeSH
- lékové postižení jater MeSH
- myši MeSH
- nemoci jater farmakoterapie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
Clinical obstetrics and gynecology
162 s. : obr., tab., přeruš.bibliogr.
- Klíčová slova
- těhotenství - diabetes - komplikace - sborníky prací,
Understanding the mechanisms regulating the development of cereal seeds is essential for plant breeding and increasing yield. However, the analysis of cereal seeds is challenging owing to the minute size, the liquid character of some tissues, and the tight inter-tissue connections. Here, we demonstrate a detailed protocol for dissection of the embryo, endosperm, and seed maternal tissues at early, middle, and late stages of barley seed development. The protocol is based on a manual tissue dissection using fine-pointed tools and a binocular microscope, followed by ploidy analysis-based purity control. Seed maternal tissues and embryos are diploid, while the endosperm is triploid tissue. This allows the monitoring of sample purity using flow cytometry. Additional measurements revealed the high quality of RNA isolated from such samples and their usability for high-sensitivity analysis. In conclusion, this protocol describes how to practically dissect pure tissues from developing grains of cultivated barley and potentially also other cereals.
The fruits of Schisandra chinensis (Turcz.) Baill. (Schisandraceae/Magnoliaceae) are a traditional Oriental medicine possessing adaptogenic and hepatoprotective activities. The lignan content in seeds and fruits of the species cultured in various European locations has been investigated. The lignans were extracted from 17 samples of the seeds with supercritical CO2 and the major components--schizandrin (1), gomisin A (2), de-oxyschizandrin (3), gomisin N (4), and wuweizisu C (5)--were quantified by HPLC. Compounds 1-5 were present in the seeds in the range 0.75-1.86, 0.13-0.90, 0.07-1.09, 0.24-1.49, and 0.01 -0.34 %, respectively. It was found that the plants cultivated in Europe accumulated comparable amounts of lignans as those of the natural distribution range.
- MeSH
- lignany MeSH
- Schisandra MeSH
- semena rostlinná MeSH
- Publikační typ
- dopisy MeSH
