spore Dotaz Zobrazit nápovědu
Flow cytometry, in combination with fluorescent staining, was used to evaluate population heterogeneity in acetone-butanol-ethanol fermentation that was carried out with type strain Clostridium beijerinckii NCIMB 8052 and non-type C. pasteurianum NRRL B-598. A combination of propidium iodide (PI) and carboxyfluorescein diacetate (CFDA), PI plus Syto-9 and bis-oxonol (BOX) alone were employed to distinguish between active and damaged cells together with simultaneous detection of spores. These strategies provided valuable information on the physiological state of clostridia. CFDA and PI staining gave the best separation of four distinct subpopulations of enzymatically active cells, doubly stained cells, damaged cells and spores. Proportional representation of cells in particular sub-regions correlated with growth characteristics, fermentation parameters such as substrate consumption and product formation in both species under different cultivation conditions.
- MeSH
- aceton metabolismus MeSH
- biomasa MeSH
- bioreaktory MeSH
- butanoly metabolismus MeSH
- Clostridium fyziologie MeSH
- energetický metabolismus * MeSH
- ethanol metabolismus MeSH
- fermentace * MeSH
- glukosa metabolismus MeSH
- mikrobiální viabilita MeSH
- průtoková cytometrie MeSH
- spory bakteriální * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
An example of bacterium, which undergoes a complex development, is the genus of Streptomyces whose importance lies in their wide capacity to produce secondary metabolites, including antibiotics. In this work, a proteomic approach was applied to the systems study of germination as a transition from dormancy to the metabolically active stage. The protein expression levels were examined throughout the germination time course, the kinetics of the accumulated and newly synthesized proteins were clustered, and proteins detected in each group were identified. Altogether, 104 2DE gel images at 13 time points, from dormant state until 5.5 h of growth, were analyzed. The mass spectrometry identified proteins were separated into functional groups and their potential roles during germination were further assessed. The results showed that the full competence of spores to effectively undergo active metabolism is derived from the sporulation step, which facilitates the rapid initiation of global protein expression during the first 10 min of cultivation. Within the first hour, the majority of proteins were synthesized. From this stage, the full capability of regulatory mechanisms to respond to environmental cues is presumed. The obtained results might also provide a data source for further investigations of the process of germination.
- MeSH
- 2D gelová elektroforéza MeSH
- antibakteriální látky biosyntéza MeSH
- hmotnostní spektrometrie MeSH
- proteom analýza MeSH
- proteosyntéza * MeSH
- regulace genové exprese u bakterií MeSH
- spory bakteriální * růst a vývoj metabolismus MeSH
- Streptomyces coelicolor * genetika růst a vývoj metabolismus MeSH
- vývojová regulace genové exprese MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
724 s. : il.
- Konspekt
- Mikrobiologie
- NLK Obory
- mikrobiologie, lékařská mikrobiologie
This review surveys whatever little is known on the influence of different environmental factors like light, temperature, nutrients, chemicals (such as plant hormones, vitamins, etc.), pH of the medium, biotic factors (such as algal extracellular substances, algal concentration, bacterial extracellular products, animal grazing and animal extracellular products), water movement, water stress, antibiotics, UV light, X-rays, ?-rays, and pollution on the spore germination in algae. The work done on the dormancy of algal spores and on the role of vegetative cells in tolerating environmental stress is also incorporated.
- MeSH
- ekosystém MeSH
- Eukaryota fyziologie účinky záření MeSH
- spory fyziologie účinky záření MeSH
- viabilita buněk MeSH
- Publikační typ
- přehledy MeSH
