The inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO3)2 NPs], which represent a highly soluble form of lead, by inhalation. We aimed to uncover the effects of their exposure on individual target organs and to reveal potential variability in the lead clearance. We examined (i) lead biodistribution in target organs using laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) and atomic absorption spectrometry (AAS), (ii) lead effect on histopathological changes and immune cells response in secondary target organs and (iii) the clearance ability of target organs. In the lungs and liver, Pb(NO3)2 NP inhalation induced serious structural changes and their damage was present even after a 5-week clearance period despite the lead having been almost completely eliminated from the tissues. The numbers of macrophages significantly decreased after 11-week Pb(NO3)2 NP inhalation; conversely, abundance of alpha-smooth muscle actin (α-SMA)-positive cells, which are responsible for augmented collagen production, increased in both tissues. Moreover, the expression of nuclear factor κB (NF-κB) and selected cytokines, such as tumor necrosis factor alpha (TNFα), transforming growth factor beta 1 (TGFβ1), interleukin 6(IL-6), IL-1α and IL-1β , displayed a tissue-specific response to lead exposure. In summary, diminished inflammatory response in tissues after Pb(NO3)2 NPs inhalation was associated with prolonged negative effect of lead on tissues, as demonstrated by sustained pathological changes in target organs, even after long clearance period.
- MeSH
- aktiny agonisté genetika imunologie MeSH
- alveolární makrofágy účinky léků imunologie patologie MeSH
- aplikace inhalační MeSH
- biologická dostupnost MeSH
- dusičnany farmakokinetika toxicita MeSH
- exprese genu MeSH
- inhalační expozice analýza MeSH
- interleukin-1alfa agonisté genetika imunologie MeSH
- interleukin-1beta agonisté genetika imunologie MeSH
- interleukin-6 agonisté genetika imunologie MeSH
- játra účinky léků imunologie patologie MeSH
- kovové nanočástice aplikace a dávkování toxicita MeSH
- látky znečišťující vzduch farmakokinetika toxicita MeSH
- myši inbrední ICR MeSH
- myši MeSH
- NF-kappa B agonisté genetika imunologie MeSH
- olovo farmakokinetika toxicita MeSH
- plíce účinky léků imunologie patologie MeSH
- poločas MeSH
- spektrofotometrie atomová MeSH
- tkáňová distribuce MeSH
- TNF-alfa agonisté genetika imunologie MeSH
- transformující růstový faktor beta1 agonisté genetika imunologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
