Cervical lymphadenopathy in children usually has an infectious or reactive etiology, but patients with persistent findings, adolescent age and accompanying systemic symptoms or abnormal laboratory findings should undergo an early biopsy. In our fifteen-year-old girl, who observed cervical lymphomegaly for 4 weeks without improvement, Staphylococcus saprophyticus was cultured from a peroperative biopsy. Coagulase-negative staphylococci (CoNS) represent the main part of the microflora of the skin and mucous membranes of humans, but they can also be used as important opportunistic pathogens.
- MeSH
- antibakteriální látky aplikace a dávkování terapeutické užití MeSH
- hnisání MeSH
- koagulasa MeSH
- krk * chirurgie patologie MeSH
- lidé MeSH
- lymfadenitida * chirurgie diagnóza terapie MeSH
- mladiství MeSH
- stafylokokové infekce diagnóza farmakoterapie MeSH
- Staphylococcus saprophyticus izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- mladiství MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Chronic wounds and their associated bacterial infections are major issues in modern health care systems. Therefore, antimicrobial resistance (AMR), treatment costs, and number of disability-adjusted life-years have gained more interest. Recently, photodynamic therapy emerged as an effective approach against resistant and naïve bacterial strains with a low probability of creating AMR. In this study, needleless electrospinning was used to produce an indocyanine green (ICG) loaded poly(d,l-lactide) nanofibrous mesh as a photoresponsive wound dressing. The non-woven mesh had a homogeneous nanofibrous structure and showed long-term hydrolytic stability at different pH values. The antimicrobial activity was tested against several bacterial strains, namely Staphylococcus saprophyticus subsp. bovis, Escherichia coli DH5 alpha, and Staphylococcus aureus subsp. aureus. Upon irradiation with a laser of a specific wavelength (λ = 810 nm), the bacterial viability was significantly reduced by 99.978% (3.66 log10), 99.699% (2.52 log10), and 99.977% (3.64 log10), respectively. The nanofibrous mesh showed good biocompatibility, which was confirmed by the proliferation of mouse fibroblasts (L929) on the surface and into deeper parts of the mesh. Furthermore, a favorable proangiogenic effect was observed in ovo using the chorioallantoic membrane assay. In general, it can be concluded that ICG loaded nanofibers as an innovative wound dressing represent a promising strategy against chronic wounds associated with skin infections.
- MeSH
- biokompatibilní materiály chemie farmakologie MeSH
- buněčné linie MeSH
- chorioalantoická membrána krevní zásobení účinky léků MeSH
- Escherichia coli účinky léků růst a vývoj MeSH
- fibroblasty cytologie účinky léků MeSH
- fotochemoterapie MeSH
- indokyanová zeleň chemie farmakologie MeSH
- mikrobiální viabilita účinky léků MeSH
- myši MeSH
- nanovlákna MeSH
- obvazy MeSH
- polyestery chemie MeSH
- proliferace buněk účinky léků MeSH
- Staphylococcus aureus účinky léků růst a vývoj MeSH
- Staphylococcus saprophyticus účinky léků růst a vývoj MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Antibiotic resistance is increasing among Staphylococcus saprophyticus strains isolated from urinary tract infection. This necessitates alternative therapies. For this, a lytic phage (vB_SsapS-104) against S. saprophyticus, which formed round and clear plaques on bacterial culture plates, was isolated from hospital wastewater and characterized. Microscopy analysis showed that it had a small head (about 50 nm), tail (about 80 nm), and a collar (about 22 nm in length and 19 nm in width) indicating to be a phage within Siphoviridae family. Phage vB_SsapS-104 showed a large latency period of about 40 min, rapid adsorption rate that was significantly enhanced by MgCl2 and CaCl2, and high stability to a wide range of temperatures and pH values. Restriction analyses demonstrated that phage consists of a double-stranded DNA with an approximate genome size of 40 Kb. BLAST results did not show high similarity (megablast) with other previously identified phages. But, in Blastn, similarity with Staphylococcus phages was observed. Phage vB_SsapS-104 represented high anti-bacterial activity against S. saprophyticus isolates in vitro as it was able to lyse 8 of the 9 clinical isolates (%88.8) obtained from a hospital in Gorgan, Iran. It was a S. saprophyticus-specific phage because no lytic activity was observed on some other pathogenic bacteria tested. Therefore, phage vB_SsapS-104 can be considered as a specific virulent phage against of S. saprophyitcus isolated from urinary tract infection. This study provided the partial genomic characterization of S. saprophyticus phage and its application against urinary tract infection associated with S. saprophyticus. This phage also can be considered as a good candidate for a therapeutic alternative in the future.
- MeSH
- antibakteriální látky farmakologie MeSH
- DNA virů MeSH
- fágová terapie MeSH
- genom virový MeSH
- hostitelská specificita MeSH
- infekce močového ústrojí mikrobiologie MeSH
- koncentrace vodíkových iontů MeSH
- latence viru MeSH
- lidé MeSH
- odpadní voda virologie MeSH
- sekvenční analýza DNA MeSH
- Siphoviridae genetika izolace a purifikace ultrastruktura MeSH
- stafylokokové bakteriofágy genetika MeSH
- stafylokokové infekce mikrobiologie MeSH
- Staphylococcus saprophyticus účinky léků virologie MeSH
- teplota MeSH
- transmisní elektronová mikroskopie MeSH
- virulence MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Írán MeSH
- MeSH
- bakteriální infekce * diagnóza MeSH
- bakteriologické techniky MeSH
- Candida albicans MeSH
- dospělí MeSH
- Escherichia coli MeSH
- laboratoře MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- mladý dospělý MeSH
- odběr biologického vzorku MeSH
- řízení kvality MeSH
- Shigella flexneri MeSH
- Staphylococcus aureus MeSH
- Staphylococcus saprophyticus MeSH
- Streptococcus pyogenes MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- tabulky MeSH
