First edition 70 stran : ilustrace ; 23 cm
The publication focuses on methods of RT-qPCR testing of viral respiratory diseases, for example COVID-19. Intended for professional public.
- MeSH
- Respiratory Tract Infections diagnosis MeSH
- Polymerase Chain Reaction methods MeSH
- COVID-19 Testing methods MeSH
- Virology MeSH
- Virus Diseases diagnosis MeSH
- Publication type
- Monograph MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- pneumologie a ftizeologie
- diagnostika
The Czech Republic, a part of the former Czechoslovakia, has been at the forefront of several research directions in virology, genetics and physiology [...].
- MeSH
- Virology * MeSH
- Publication type
- Editorial MeSH
- Geographicals
- Czech Republic MeSH
Up to 170 tick-borne viruses (TBVs) have been identified to date. However, there is a paucity of information regarding TBVs and their interaction with respective vectors, limiting the development of new effective and urgently needed control methods. To overcome this gap of knowledge, it is essential to reproduce transmission cycles under controlled laboratory conditions. In this study we assessed an artificial feeding system (AFS) and an immersion technique (IT) to infect Ixodes ricinus ticks with tick-borne encephalitis (TBE) and Kemerovo (KEM) virus, both known to be transmitted predominantly by ixodid ticks. Both methods permitted TBEV acquisition by ticks and we further confirmed virus trans-stadial transmission and onward transmission to a vertebrate host. However, only artificial feeding system allowed to demonstrate both acquisition by ticks and trans-stadial transmission for KEMV. Yet we did not observe transmission of KEMV to mice (IFNAR-/- or BALB/c). Artificial infection methods of ticks are important tools to study tick-virus interactions. When optimally used under laboratory settings, they provide important insights into tick-borne virus transmission cycles.
- MeSH
- Arachnid Vectors physiology virology MeSH
- Host-Pathogen Interactions MeSH
- Ixodes physiology virology MeSH
- Encephalitis, Tick-Borne transmission virology MeSH
- Humans MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Orbivirus physiology MeSH
- Reoviridae Infections transmission virology MeSH
- Virology methods MeSH
- Encephalitis Viruses, Tick-Borne physiology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Anthropology MeSH
- COVID-19 * MeSH
- Communicable Diseases virology MeSH
- Coronavirus Infections MeSH
- Humans MeSH
- Animal Diseases MeSH
- Pandemics MeSH
- Disease Transmission, Infectious MeSH
- Virology MeSH
- Viruses MeSH
- Check Tag
- Humans MeSH
- Publication type
- Popular Work MeSH
- Interview MeSH
