A wide range of articles describe the role of different probiotics in the prevention or treatment of various diseases. However, currently, the focus is shifting from whole microorganisms to their easier-to-define components that can confer similar or stronger benefits on the host. Here, we aimed to describe polysaccharide B.PAT, which is a surface antigen isolated from Bifidobacterium animalis ssp. animalis CCDM 218 and to understand the relationship between its structure and function. For this reason, we determined its glycerol phosphate-substituted structure, which consists of glucose, galactose, and rhamnose residues creating the following repeating unit: To fully understand the role of glycerol phosphate substitution on the B.PAT function, we prepared the dephosphorylated counterpart (B.MAT) and tested their immunomodulatory properties. The results showed that the loss of glycerol phosphate increased the production of IL-6, IL-10, IL-12, and TNF-α in bone marrow dendritic cells alone and after treatment with Lacticaseibacillus rhamnosus GG. Further studies indicated that dephosphorylation can enhance B.PAT properties to suppress IL-1β-induced inflammatory response in Caco-2 and HT-29 cells. Thus, we suggest that further investigation of B.PAT and B.MAT may reveal distinct functionalities that can be exploited in the treatment of various diseases and may constitute an alternative to probiotics.
- MeSH
- bakteriální polysacharidy farmakologie chemie izolace a purifikace MeSH
- Bifidobacterium animalis * chemie MeSH
- buňky HT-29 MeSH
- Caco-2 buňky MeSH
- cytokiny metabolismus MeSH
- dendritické buňky účinky léků imunologie metabolismus MeSH
- fosforylace účinky léků MeSH
- imunologické faktory farmakologie chemie izolace a purifikace MeSH
- Lacticaseibacillus rhamnosus chemie MeSH
- lidé MeSH
- myši MeSH
- probiotika farmakologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Here, we compared the abilities of polysaccharides L900/2 and L900/3, which were previously isolated from Lactobacillus rhamnosus LOCK 0900, to modulate the immune response to bystander antigens in a mouse model of ovalbumin (OVA) sensitization. In vivo, both polysaccharides reduced the levels of OVA-specific IgE, IgE-dependent basophil degranulation and IgG2a antibodies, but had no effect on the levels of OVA-specific IgA or IgG1. Interestingly, both polysaccharides triggered recall cellular responses with distinct properties. L900/3 significantly suppressed the OVA-induced upregulations of IL-4, IL-5, IL-10 and IL-13 in re-stimulated spleen cells and mesenteric lymph nodes. Our findings support and expand on our previous in vitro studies by demonstrating that polymer L900/3 might modulate the Th1/Th2 balance and could be a promising candidate molecule for preventing allergic sensitization.
- MeSH
- alergeny imunologie MeSH
- bakteriální polysacharidy aplikace a dávkování izolace a purifikace MeSH
- bazofily imunologie MeSH
- cytokiny biosyntéza MeSH
- degranulace buněk MeSH
- imunizace * MeSH
- imunoglobulin E krev MeSH
- imunoglobulin G krev MeSH
- imunologické faktory aplikace a dávkování izolace a purifikace MeSH
- Lacticaseibacillus rhamnosus chemie MeSH
- myši MeSH
- serpiny imunologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
( GOS) sú ne stráviteľné sacharidy známe ako prebiotiká – môžu stimulovať rast a aktivitu prospešnej črevnej mikroflóry ( bifidobaktérie a laktobacily ) a inhibujú rast potenciálne patogénnych baktérií. GOS sú komerčne vyrábané z laktózy pomocou mikrobiálnej β - galaktozidázy. Tieto enzýmy katalyzujú hydrolýzu , ako aj transgalaktozyláciu laktózy . Pomer medzi týmito reakciami sa líši v závislosti od zdroja enzýmu a podmienok enzýmovej . V poslednom období, inovačné stratégie pre syntézu GOS zahŕňajú imobilizáciu enzýmov a využitie srvátky ako základnej suroviny.
Galacto-oligosaccharides (GOS) are non-digestible carbohydrates know as a prebiotic – can stimulate growth and activity of beneficial intestinal microflora (bifidobacteria and lactobacilli) and inhibit the growth of potencially pathogenic bacteria. GOS are commercially produced from lactose using microbial β -galactosidases. These enzymes catalyze hydrolysis as well as the transgalactosylation of lactose. The proportion between these reactions varies depending on the source of the enzyme and conditions of enzymatic reaction. More recently, an innovative strategies for GOS synthesis are immobilization of enzymes and usage of whey as a source of the lactose.
- MeSH
- antigeny bakteriální * imunologie izolace a purifikace klasifikace MeSH
- Bacteria MeSH
- bakteriální polysacharidy * imunologie izolace a purifikace klasifikace MeSH
- bakteriální struktury imunologie klasifikace patogenita MeSH
- bakteriologie trendy MeSH
- lidé MeSH
- stafylokokový protein A imunologie izolace a purifikace MeSH
- Staphylococcus aureus * imunologie metabolismus patogenita MeSH
- statistika jako téma MeSH
- Check Tag
- lidé MeSH
- MeSH
- agar MeSH
- antisérum MeSH
- autoradiografie MeSH
- bakteriální polysacharidy izolace a purifikace MeSH
- brom MeSH
- chemická precipitace MeSH
- elektroforéza MeSH
- ethanol MeSH
- imunochemie MeSH
- imunodifuze MeSH
- imunoelektroforéza MeSH
- izotopy fosforu MeSH
- králíci MeSH
- kyanidy MeSH
- kyselina trichloroctová MeSH
- lipopolysacharidy chemie MeSH
- Shigella imunologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
