Microscopic diagnosis of equine piroplasmoses, caused by Theileria equi and Babesia caballi, is hindered by low parasitaemia during the latent phase of the infections. However, this constraint can be overcome by the application of PCR followed by sequencing. Out of 288 animals examined, the piroplasmid DNA was detected in 78 (27·1%). Multiplex PCR indicated that T. equi (18·8%) was more prevalent than B. caballi (7·3%), while mixed infections were conspicuously absent. Sequences of 69 PCR amplicons obtained by the 'catch-all' PCR were in concordance with those amplified by the multiplex strategy. Computed minimal adequate model analyses for both equine piroplasmid species separately showed a significant effect of host species and age in the case of T. equi, while in the B. caballi infections only the correlation with host sex was significant. Phylogenetic analyses inferred the occurrence of three genotypes of T. equi and B. caballi. Moreover, a novel genotype C of B. caballi was identified. The dendrogram based on obtained sequences of T. equi revealed possible speciation events. The infections with T. equi and B. caballi are enzootic in all ecozones of Jordan and different genotypes circulate wherever dense horse population exists.

• MeSH
• Babesia klasifikace   genetika   izolace a purifikace   MeSH
• babezióza epidemiologie   parazitologie   MeSH
• Equidae parazitologie   MeSH
• fylogeneze MeSH
• genetická variace * MeSH
• genotyp MeSH
• koně MeSH
• molekulární sekvence - údaje MeSH
• multiplexová polymerázová řetězová reakce veterinární   MeSH
• nemoci koní epidemiologie   parazitologie   MeSH
• parazitemie veterinární   MeSH
• prevalence MeSH
• protozoální DNA chemie   genetika   MeSH
• sekvence nukleotidů MeSH
• sekvenční analýza DNA veterinární   MeSH
• skot MeSH
• Theileria klasifikace   genetika   izolace a purifikace   MeSH
• theilerióza epidemiologie   parazitologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• skot MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Jordánsko MeSH

DNA of two species of piroplasmids was detected in dromedaries during a survey of blood protozoans in Jordan between 2007 and 2009. Ten clinically healthy camels (10%) originating from three Jordanian districts were found, using a PCR assay, to harbor Theileria or Babesia species in their blood and no mix infection was determined. Analysis of the partial 18S rRNA gene sequences of these parasites allowed their unambiguous identification as equine piroplasmids Babesia caballi (n=6) and Theileria equi (n=4). In case of latter species, a novel genotype was found in horses. This first molecular-based species determination of piroplasmids from camels further contributes to the growing evidence of low host specificity of piroplasmids.

• MeSH
• Babesia klasifikace   genetika   izolace a purifikace   MeSH
• babezióza diagnóza   epidemiologie   veterinární   MeSH
• fylogeneze MeSH
• koně MeSH
• nemoci koní epidemiologie   parazitologie   MeSH
• polymerázová řetězová reakce veterinární   MeSH
• Theileria klasifikace   genetika   izolace a purifikace   MeSH
• theilerióza diagnóza   epidemiologie   MeSH
• velbloudi parazitologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Jordánsko MeSH