A rapid, high-throughput method employing ultra-performance liquid chromatography with tandem quadrupole mass spectrometry (UPLC-MS/MS) was developed and optimized for simultaneous quantification and confirmation of 64 pesticide residues and their toxic metabolites in fruit extracts prepared by a buffered QuEChERS procedure. The total time required for UPLC-MS/MS analysis was 8 min plus 2 min for re-equilibration to the initial UPLC conditions. Performance characteristics were determined for apple extracts spiked at 10 microg kg(-1). The repeatability of measurements expressed as relative standard deviations was in the range 1.5-13% at this level for most analytes. Thanks to very low limits of quantification (<10 microg kg(-1)for the majority of pesticides), an optimized method allows for the reliable control of not only common maximum residue limits (MRLs) set by European Union regulation for various pesticides/fruit combinations, but also of a uniform MRL of 10 microg kg(-1)endorsed for baby food.

• MeSH
• časové faktory MeSH
• Evropská unie MeSH
• financování organizované MeSH
• kalibrace MeSH
• kojenec MeSH
• lidé MeSH
• Malus chemie   MeSH
• maximální přípustná koncentrace MeSH
• potrava pro kojence normy   MeSH
• referenční standardy MeSH
• reprodukovatelnost výsledků MeSH
• rezidua pesticidů analýza   MeSH
• řízení kvality MeSH
• tandemová hmotnostní spektrometrie metody   MeSH
• vysokoúčinná kapalinová chromatografie metody   MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• Publikační typ
• validační studie MeSH

A total of 19 pesticide preparations were used according to agricultural practice in six trials in apple orchards. Using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), premature Golden Delicious apples collected 64, 50, 36 days before harvest and mature fruit were examined for residues of active ingredients. No residues of triflumuron, triazamate, chlorpyrifos, etofenprox, fenoxycarb, kresoxim-methyl, cyprodinyl, difenoconazole or thiram were detected in the first sampling. Also, the levels of chlorpyrifos-methyl, penconazole, tebuconazole and tolylfluanid dropped during the pre-harvest interval. Detectable residues of pyridaben, thiacloprid, trifloxystrobin and tetraconazole in harvested fruits were below 0.01 mg kg(-1), which is the maximum concentration of residues acceptable by baby-food producers in any raw material. The only residues exceeding this concentration were captan and teflubenzuron. Based on the data, farmers can choose pesticides for optimal treatment of plants, while enabling growth of a safe crop suitable for baby-food production.

• MeSH
• analýza potravin metody   MeSH
• chromatografie kapalinová metody   MeSH
• financování organizované MeSH
• kojenec MeSH
• kontaminace potravin analýza   prevence a kontrola   MeSH
• lidé MeSH
• Malus chemie   MeSH
• ovoce chemie   normy   MeSH
• pesticidy normy   MeSH
• plynová chromatografie s hmotnostně spektrometrickou detekcí metody   MeSH
• potrava pro kojence normy   MeSH
• rezidua pesticidů analýza   MeSH
• tandemová hmotnostní spektrometrie metody   MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH